Purification and biochemical characterization of phytase from Bacillus cereus isolated from gastrointestinal tract of African giant snail (Achatina fulica).

Phytases are specialized enzymes meant for phytic acid degradation. They possess ability to prevent phytic acid indigestion, including its attendant environmental pollution. This study was aimed at investigating biochemical properties of purified phytase of B.

Engineered sp. by chemical mutagen produces thermostable and acido-alkalophilic endo-1,4-β-mannanases for improved industrial biocatalyst.

This study reported physicochemical properties of purified endo-1,4-β-mannanase from the wild type, sp. and its most promising chemical mutant. The crude enzymes from fermentation of wild and mutant bacteria were purified by ammonium sulfate precipitation, ion exchange and gel-filtration chromatography followed by an investigation of the physicochemical properties of purified wild and mutant enzymes.

Properties of a neutral, thermally stable and surfactant-tolerant pullulanase from worker termite gut-dwelling as potential for industrial applications.

The gut of termite has been observed to host communities of bacteria which exhibited pullulan-degrading ability. displayed maximum pullulanase (a debranching enzyme) activity and it was therefore selected for production, purification and characterization of pullulanase which was the aim of the study. The crude enzyme obtained from the pullulanase production medium was subjected to ammonium sulphate precipitation, ion exchange and gel-filtration chromatography and the physicochemical properties of the purified was thereafter characterized.

Biochemical studies of enzyme-induced browning of African bush mango () fruit pulp.

The purpose of this study was to examine the biochemical properties of African bush mango () pulp PPO. PPO was purified from fruit pulp in three steps and characterized. A purification fold of 343 with specific activity of 216 U/mg and 13% recovery were obtained as well as molecular weight of 32.