Network pharmacology and molecular docking-based identification of drug candidates and key targets of for colorectal cancer treatment.

Colorectal cancer (CRC) is a type of cancer with high morbidity and mortality in several developing and developed countries of the world. Its mortality and morbidity are predicted to increase over the next decade, hence, efforts aimed at combating it have remained unabated. In the context of its treatment, the use of chemotherapeutics is often limited by challenges including cost-ineffectiveness, side effects, and drug resistance.

Identification of a 1, 8-naphthyridine-containing compound endowed with the inhibition of p53-MDM2/X interaction signaling: a computational perspective.

Various studies have established that molecules specific for MDMX inhibition or optimized for dual inhibition of p53-MDM2/MDMX interaction signaling are more suitable for activating the Tp53 gene in tumor cells. Nevertheless, there are sparse numbers of approved molecules to treat the health consequences brought by the lost p53 functions in tumor cells. Consequently, this study explored the potential of a small molecule ligand containing 1, 8-naphthyridine scaffold to act as a dual inhibitor of p53-MDM2/X interactions using computational methods.

Comparative study of the reproductive toxicity and modulation of enzyme activities by crude oil-contaminated soil before and after bioremediation.

Contamination of soil with crude oil is a serious ecological problem with potential adverse public health effects. This study assessed the germ cell toxicity of simulated leachates from crude oil-contaminated soil before and after bioremediation using the murine sperm abnormality assay, sperm count, and testes histopathology. The levels of Total Testosterone (TT), Follicle Stimulating Hormone (FSH), and Luteinizing Hormone (LH); and activities of catalase (CAT), alkaline phosphatase (ALP), superoxide dismutase (SOD), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were determined.

Levels of polybrominated diphenyl ethers (PBDEs) in water and sediment from open city drains in Makurdi Metropolitan Area, North Central Nigeria.

Polybrominated diphenyl ethers (PBDEs) have been classified as persistent organic pollutants (POPs) as well as endocrine-disrupting compounds (EDCs). This study determined the concentrations of six PBDE congeners (BDE 47, BDE 99, BDE 100, BDE 153, BDE 154, and BDE 183) in water and sediment samples from open city drains, in the Makurdi Metropolitan Area, North Central Nigeria, using gas chromatogragh-mass spectrometer. These congeners are components of the penta- and octa-BDE formulations that have been banned by the European Union.

In silico design and validation of a highly degenerate primer pair: a systematic approach.

Background: The techniques of amplifying genetic materials have enabled the extensive study of several biological activities outside the biological milieu of living systems. More recently, this approach has been extended to amplify population of genes, from evolutionarily related gene family for detection and evaluation of microbial consortial with several unique potentialities (e.g.

The occurrence of brominated flame retardants in the atmosphere of Gauteng Province, South Africa using polyurethane foam passive air samplers and assessment of human exposure.

Polyurethane foam passive samplers were deployed between May 2016 and January 2017 to evaluate concentrations of polybrominated diphenyl ethers (PBDEs), selected alternative flame retardants (AFRs) and total hexabromocyclododecane (HBCDD) (sum of α-, β-, and γ-HBCDD). The PUF air samplers were deployed in semi-urban, urban, industrial and landfill sites in Gauteng Province, South Africa. The acquired results presented a clear semi urban-industrial-urban-landfill concentration gradient for all BFRs measured.

Temporal trends of Dechlorane Plus in air and precipitation around the North American Great Lakes.

Dechlorane Plus (DP) is a chlorinated flame retardant manufactured only in Niagara Falls, New York and in Huai'an, China. To determine if the environmental levels of this compound were changing significantly, we measured the long-term temporal trends of its concentrations near the Great Lakes between 2005 and 2015 using air (vapor + particle phase) samples (N = 1047) and precipitation samples (N = 449). We used a multiple linear regression model of DP concentrations to isolate the variabilities due to sampling date and population near the sampling site.

Atmospheric concentrations of hexabromocyclododecane (HBCDD) diastereomers in the Great Lakes region.

The concentrations of α-, β-, and γ-hexabromocyclododecane (HBCDD) diastereomers in atmospheric particle phase samples were determined at four United States Integrated Atmospheric Deposition Network (IADN) sites located in the North American Great Lakes basin collected between 1 January and 31 December 2014. The concentrations ranged from 0.37 to 8.

Determination of legacy and novel brominated flame retardants in dust from end of life office equipment and furniture from Pretoria, South Africa.

Indoor dust is known to be a source of human exposure to brominated flame retardants (BFRs) and these consists of the legacy polybrominated diphenyl ethers (PBDEs), total hexabromocyclododecane (T-HBCDD) and the "Novel or alternate" Brominated flame retardants (NBFRs). In this study, x-ray fluorescence (XRF) analyser was employed to measure elemental bromine contents in office furniture and electronics as the first indication of the possible presence of BFRs. To investigate the possible BFRs present, a total of 21 dust samples were collected from surfaces of electronic equipment and office furniture and were analysed using gas chromatography-mass spectrometry (GC-MS).

Soil concentrations of polybrominated diphenyl ethers and trace metals from an electronic waste dump site in the Greater Accra Region, Ghana: Implications for human exposure.

Unregulated electronic waste (e-waste) recycling operations have become a significant environmental issue as well as human health risk in developing countries across the world. The present study evaluated the extent of pollution in Agbogbloshie e-waste recycling site in Accra, Ghana. The concentrations of polybrominated diphenyl ethers (PBDEs) and some selected trace metals were determined using gas chromatography electron impact ionization mass spectrometry and flame atomic absorption spectrophotometry, respectively.

Effects of Temperature and pH on the Activities of Catechol 2,3-dioxygenase Obtained from Crude Oil Contaminated Soil in Ilaje, Ondo State, Nigeria.

Enrichment technique was employed for the isolation of the crude oil degrading bacteria. The isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed.

Concentration of novel brominated flame retardants and HBCD in leachates and sediments from selected municipal solid waste landfill sites in Gauteng Province, South Africa.

In this study leachate and sediment samples were collected from six municipal solid waste landfill sites across Gauteng Province in South Africa to determine the levels of 2-ethylhexyl 2,3,4,5 tetrabromobenzoate (EH-TBB), 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ethane (DBDPE), bis(2-ethylhexyl)-3,4,5,6-tetrabromo-phthalate (BEH-TEBP) and hexabromocyclododecane (HBCD). Soxhlet as well as liquid-liquid extraction were employed for sediment and leachates respectively followed by GC-EIMS analysis. Concentrations of novel brominated flame retardants (NBFRs) ranged from below detection ( View Article and Find Full Text PDF

Levels of polybrominated diphenyl ethers in indoor dust and human exposure estimates from Makurdi, Nigeria.

Information on polybrominated diphenyl ethers (PBDEs) in the indoor environment in developing countries is still relatively scarce. In this study, house (n=10) and office (n=11) dusts samples collected from Makurdi, Benue State Nigeria were extracted and analysed for most abundant PBDEs congeners in the environment. Soxhlet extraction followed by GC-EIMS was employed for the measurement of PBDEs (BDE-47, -100, -99, -154, -153, -183 and -209).

Influence of physicochemical and chemical parameters on polybrominated diphenyl ethers in selected landfill leachates, sediments and river sediments from Gauteng, South Africa.

Polybrominated diphenyl ethers (PBDEs) are known to be persistent, endocrine disruptors and bioaccumulative and can cause adverse health effects in animals and humans. In this study, river and landfill sediment samples were collected from selected rivers and municipal solid waste landfill (MSWL) sites across Gauteng Province in South Africa to determine the levels of common PBDEs (BDE-17, BDE-47, BDE-99, BDE-100, BDE-153, BDE-154, BDE-183 and BDE-209). The mean and median concentrations of Σ8 PBDEs from river sediment samples was 2.

Determination of brominated flame retardants in Jukskei River catchment area in Gauteng, South Africa.

Brominated flame retardants (BFRs) are considered to be environmental pollutants due to their toxicity, persistence and ubiquity in the environment. Little information is known about the presence of brominated flame retardants in South Africa's water systems. Therefore, this study examined and compared different extraction methods (liquid-liquid (LL) vs.

Concentrations of polybrominated diphenyl ethers in sediments from Jukskei River, Gauteng, South Africa.

This study determined concentration levels of polybrominated diphenyl ethers (PBDEs) in sediment samples collected from Jukskei River in South Africa. Final extracts, after concentration and dilution to 200 μL were analyzed by injecting 1 μL in the GC-ECD and GC-MS. Results obtained showed good recoveries (73%-114%, with RSD < 17%).