Improved enzyme production on corncob hydrolysate by a xylose-evolved cell factory.

The global shift from the usage of crude oil in bio-production is receiving much attention owing to environmental concern associated with fossil fuel. Lignocellulosic biomass (LB) is a good carbon candidate for bio-production because it is environmental-friendly. Corncob being one of such LB is rich in glucose and xylose, which can be utilized for bio-production.

Microbial redemption of "evil" days: a global appraisal to food security.

Without refute, a sustainable global food security can only be achieved when all folks have physical, social and economic access to safe, nutritious, and sufficient supply of food to meet their dietary needs and food preferences for healthy life. To this end, quest to achieve this dream has been on course since 1970s as evident by the establishment of a committee on food security in 1975 by the UN World Food Conference to oversee and make developmental difference in food security. Interestingly, 2019 Global Hunger Index revealed transition in global hunger from serious to moderate with 31% decline in global hunger since 2000, and hence depicting enhanced food security.

Engineering unnatural methylotrophic cell factories for methanol-based biomanufacturing: Challenges and opportunities.

Methanol is a very promising feedstock alternative to sugar-based raw materials for biomanufacturing because it does not compete with food production, is abundant and potentially sustainable in the future. Although methylotrophic fermentations have been practiced for decades, their applications are limited by technical drawbacks and insufficient knowledge of the physiology and metabolic regulation of native methylotrophs. Synthetic biology offers great opportunities for engineering efficient methylotrophic microbial cell factories by enabling non-methylotrophic model organisms to utilize methanol via the introduction of C1 utilization pathways.

Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris.

Objectives: To systematically explore the effects of overexpressing Hac1p homologues from different sources on protein secretion in Pichia pastoris system.

Results: Effects of Hac1p homologues encompassing P. pastoris (PpHac1p), S.