'Feeling Hot': Exploring the feasibility of nocturnal erection detection through penile temperature measurements.

Objectives: The observational 'Feeling Hot' study aims to evaluate the feasibility of employing overnight penile temperature measurements for the detection of nocturnal erections, thereby contributing to the advancement and modernization of a non-invasive diagnostic system for erectile dysfunction.

Subjects/patients And Methods: In this proof-of-concept study, 10 healthy men aged 20-25 were recruited, following the methodology outlined in the 'Staying Hot' study by Torenvlied et al. Participants underwent ambulatory overnight penile temperature measurements concurrent with RigiScan recordings.

'Staying Hot': Investigating the influence of overnight conditions on the penile skin temperature during male sexual arousal-A novel methodology for nocturnal erection detection.

Objective: The objective of this study is to assess the impact of overnight environmental conditions on erectile penile temperature within a controlled setting, with the aim of investigating the feasibility of using temperature measurements for nocturnal erection detection in erectile dysfunction diagnostics.

Subjects/patients And Methods: We conducted a proof-of-concept study involving 10 healthy male participants aged 20 to 25. The study was carried out at the Department of Urology, St.

Microfluidic Impedance Cytometry for Single-Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae.

Calcifying algae, like coccolithophores, greatly contribute to the oceanic carbon cycle and are therefore of particular interest for ocean carbon models. They play a key role in two processes that are important for the effective CO flux: The organic carbon pump (photosynthesis) and the inorganic carbon pump (calcification). The relative contribution of calcification and photosynthesis can be measured in algae by the amount of particulate inorganic carbon (PIC) and particulate organic carbon (POC).

Supervised machine learning in microfluidic impedance flow cytometry for improved particle size determination.

The assessment of particle and cell size in electrical microfluidic flow cytometers has become common practice. Nevertheless, in flow cytometers with coplanar electrodes accurate determination of particle size is difficult, owing to the inhomogeneous electric field. Pre-defined signal templates and compensation methods have been introduced to correct for this positional dependence, but are cumbersome when dealing with irregular signal shapes.

C-Term Reduction in 3 μm Open-Tubular High-Aspect-Ratio Channels in AC-EOF Vortex Chromatography Operation.

The performance of liquid chromatography operation in open-tubular channels, the ideal chromatographic column format, is limited by slow mass transport between the mobile and stationary phase. We recently introduced a lateral mixing methodology ("vortex chromatography") to reduce Taylor-Aris dispersion by employing (small) AC-EOF (alternating current electroosmotic flow) fields oriented perpendicular to the conventionally applied, axially oriented pressure gradient, resulting in the reduction of the C-term by a factor of 3, studied in 40 × 20 μm (aspect ratio (AR) = 2) channels under unretained conditions. In the present contribution, a further increased performance gain for channel dimensions relevant for chromatographic applications is demonstrated.

Miniaturized Control of Acidity in Multiplexed Microreactors.

The control of acidity drives the assembly of biopolymers that are essential for a wide range of applications. Its miniaturization can increase the speed and the possibilities of combinatorial throughput for their manipulation, similar to the way that the miniaturization of transistors allows logical operations in microelectronics with a high throughput. Here, we present a device containing multiplexed microreactors, each one enabling independent electrochemical control of acidity in ∼2.

Label-Free Microfluidic Impedance Cytometry for Acrosome Integrity Assessment of Boar Spermatozoa.

Microfluidics and lab-on-chip technologies have been used in a wide range of biomedical applications. They are known as versatile, rapid, and low-cost alternatives for expensive equipment and time-intensive processing. The veterinary industry and human fertility clinics could greatly benefit from label-free and standardized methods for semen analysis.

Planar Junctionless Field-Effect Transistor for Detecting Biomolecular Interactions.

Label-free field-effect transistor-based immunosensors are promising candidates for proteomics and peptidomics-based diagnostics and therapeutics due to their high multiplexing capability, fast response time, and ability to increase the sensor sensitivity due to the short length of peptides. In this work, planar junctionless field-effect transistor sensors (FETs) were fabricated and characterized for pH sensing. The device with SiO gate oxide has shown voltage sensitivity of 41.

Application of generalized dispersion theory to vortex chromatography.

Acoustically induced secondary flows are applied to enhance lateral mass transfer beyond the relatively slow diffusion. This has the goal to reduce convective axial dispersion and the resulting band broadening which, in turn, limits the performance of column chromatography. Traditional approaches based on Taylor-Aris model are limited to one-dimensional rectilinear (unidirectional) tube- or channel-flows.

Determining Particle Size and Position in a Coplanar Electrode Setup Using Measured Opacity for Microfluidic Cytometry.

Microfluidic impedance flow cytometers enable high-throughput, non-invasive, and label-free detection of single-cells. Cytometers with coplanar electrodes are easy and cheap to fabricate, but are sensitive to positional differences of passing particles, owing to the inhomogeneous electric field. We present a novel particle height compensation method, which employs the dependence of measured electrical opacity on particle height.

Microfluidic Electrochemistry Meets Trapped Ion Mobility Spectrometry and High-Resolution Mass Spectrometry-In Situ Generation, Separation, and Detection of Isomeric Conjugates of Paracetamol and Ethoxyquin.

Over the last 3 decades, electrochemistry (EC) has been successfully applied in phase I and phase II metabolism simulation studies. The electrochemically generated phase I metabolite-like oxidation products can react with selected reagents to form phase II conjugates. During conjugate formation, the generation of isomeric compounds is possible.

Inducing AC-electroosmotic flow using electric field manipulation with insulators.

Classically, the configuration of electrodes (conductors) is used as a means to determine AC-electroosmotic flow patterns. In this paper, we use the configuration of insulator materials to achieve AC-electroosmotic flow patterning in a novel approach. We apply AC electric fields between parallel electrodes situated on the top and bottom of a microfluidic channel and separated by an insulating material.

Monitoring Contractile Cardiomyocytes via Impedance Using Multipurpose Thin Film Ruthenium Oxide Electrodes.

A ruthenium oxide (RuOx) electrode was used to monitor contractile events of human pluripotent stem cells-derived cardiomyocytes (hPSC-CMs) through electrical impedance spectroscopy (EIS). Using RuOx electrodes presents an advantage over standard thin film Pt electrodes because the RuOx electrodes can also be used as electrochemical sensor for pH, O, and nitric oxide, providing multisensory functionality with the same electrode. First, the EIS signal was validated in an optically transparent well-plate setup using Pt wire electrodes.

Measuring Both pH and O with a Single On-Chip Sensor in Cultures of Human Pluripotent Stem Cell-Derived Cardiomyocytes to Track Induced Changes in Cellular Metabolism.

studies which focus on cellular metabolism can benefit from time-resolved readouts from the living cells. pH and O concentration are fundamental parameters upon which cellular metabolism is often inferred. This work demonstrates a novel use of a ruthenium oxide (RuO) electrode for studies.

Coccolithophore calcification studied by single-cell impedance cytometry: Towards single-cell PIC:POC measurements.

Since the industrial revolution 30% of the anthropogenic CO is absorbed by oceans, resulting in ocean acidification, which is a threat to calcifying algae. As a result, there has been profound interest in the study of calcifying algae, because of their important role in the global carbon cycle. The coccolithophore Emiliania huxleyi is considered to be globally the most dominant calcifying algal species, which creates a unique exoskeleton from inorganic calcium carbonate platelets.

Reduction of Taylor-Aris dispersion by lateral mixing for chromatographic applications.

Chromatographic columns are suffering from Taylor-Aris dispersion, especially for slowly diffusing molecules such as proteins. Since downscaling the channel size to reduce Taylor-Aris dispersion meets fundamental pressure limitations, new strategies are needed to further improve chromatography beyond its current limits. In this work we demonstrate a method to reduce Taylor-Aris dispersion by lateral mixing in a newly designed silicon AC-electroosmotic flow mixer.

Determining the antioxidant properties of various beverages using staircase voltammetry.

Antioxidants are molecules that neutralize reactive oxygen species in the human body, reportedly reducing the risk of cancer and cardiovascular diseases. With multiple dietary products being advertised by their assumed high antioxidant concentration, the need for a proper way of analyzing antioxidant containing beverages becomes apparent. In this research, the antioxidant nature of teas, wines and (superfood) juices is investigated using staircase voltammetry (SV).

Spectroelectrochemistry, the future of visualizing electrode processes by hyphenating electrochemistry with spectroscopic techniques.

The combination of electrochemistry and spectroscopy, known as spectroelectrochemistry (SEC), is an already established approach. By combining these two techniques, the relevance of the data obtained is greater than what it would be when using them independently. A number of review papers have been published on this subject, mostly written for experts in the field and focused on recent advances.

The influence of geometry and other fundamental challenges for bio-sensing with field effect transistors.

We present a review of field effect transistors (FET) from the point of view of their applications to label-free sensing in the era of genomics and proteomics. Here, rather than a collection of Bio-FET achievements, we propose an analysis of the different issues hampering the use of these devices into clinical applications. We make a particular emphasis on the influence of the sensor geometry in the phenomena of mass transport of analytes, which is a topic that has been traditionally overlooked in the analysis and design of biosensors, but that plays a central role in the achievement of low limits of detection.

Microfluidics and catalyst particles.

In this review article, we discuss the latest advances and future perspectives of microfluidics for micro/nanoscale catalyst particle synthesis and analysis. In the first section, we present an overview of the different methods to synthesize catalysts making use of microfluidics and in the second section, we critically review catalyst particle characterization using microfluidics. The strengths and challenges of these approaches are highlighted with various showcases selected from the recent literature.

Ion Concentration Polarization for Microparticle Mesoporosity Differentiation.

Microparticle porosity is normally determined in bulk manner providing an ensemble average that hinders establishing the individual role of each microparticle. On the other hand, single particle characterization implies expensive technology. We propose to use ion concentration polarization to measure differences in mesoporosity at the single particle level.

High Aspect Ratio Fin-Ion Sensitive Field Effect Transistor: Compromises toward Better Electrochemical Biosensing.

The development of next generation medicines demands more sensitive and reliable label-free sensing able to cope with increasing needs of multiplexing and shorter times to results. Field effect transistor-based biosensors emerge as one of the main possible technologies to cover the existing gap. The general trend for the sensors has been miniaturization with the expectation of improving sensitivity and response time but presenting issues with reproducibility and noise level.

Single step fabrication of Silicon resistors on SOI substrate used as Thermistors.

Temperature sensing is one of the important features of Micro Electro Mechanical Systems and a monolithic integration provides advantages for both fabrication simplicity and performance. The use of Silicon On Insulator substrates allows simple fabrication of integrated wires that can be used as thermistors. We fabricated rectangular and triangular silicon wires with different dimensions in a single step fabrication process based on the wet etching of a <110> Silicon On Insulator substrate.

Gradient in the electric field for particle position detection in microfluidic channels.

In this work, a new method to track particles in microfluidic channels is presented. Particle position tracking in microfluidic systems is crucial to characterize sorting systems or to improve the analysis of cells in impedance flow cytometry studies. By developing an electric field gradient in a two parallel electrode array the position of the particles can be tracked in one axis by impedance analysis.