Collection and physiological measurements of peripheral lymph and interstitial fluid in man.

Methods used for collection and measurement of physiological parameters of peripheral lymph and interstitial fluid in man have been described, basing on the experience of lymphological groups from Warsaw and Oslo and on data from the relevant literature. The review consists of description of methods for chronic cannulation of leg lymphatics, collection of lymph from skin lymphatic vessels, measurement of peripheral lymph flow, lymph hydrostatic and oncotic pressures, collection of interstitial fluid with the capsular, liquid-paraffin cavity, wick and skin window methods, interstitial fluid hydrostatic and oncotic pressure measurement.

Radiological evidence of lymphatic drainage of bone marrow cavity in long bones.

Injection of Lipiodol UF into the distal epiphyses of tibia and femur in the dog was followed within several minutes by visualization of lymphatics draining the bone. Some of the radioopaque material was retained in the bone marrow cavity and tiny lymph vessels originating in that area could be seen. Contrast medium filled also the popliteal lymph node.

Flow and composition of leg lymph in normal men during venous stasis, muscular activity and local hyperthermia.

Changes in normal human leg lymph protein concentration, output, and lymph flow/lymph protein concentration relationship, as well as lactate dehydrogenase and alkaline phosphatase activity were followed during procedures known to increase capillary filtration as venous stasis, muscular exercise and warming of tissues. Lymph flow increased by 83% during two hour ergometer cycling, and by 117% during two hour warm water foot bath. During a two hour period of venous stasis lymph flow dropped by 50%.

Twenty-four hour variation in flow and composition of leg lymph in normal men.

The twenty-four hour variation in concentration and output of total protein, lactate dehydrogenase and alkaline phosphatase has been studied in the peripheral lymph from the legs of 5 healthy volunteers over a period of five days. The highest concentration of these proteins was found in lymph collected during the first two hours after a night's rest. During the day a continuous decrease in concentration occurred without any direct correlation to lymph flow.

Anaesthesia for orthotopic allogenic liver transplantation in pigs.

Anaesthesia with a mixture of halothane, nitrous oxide and oxygen was used for allogenic liver transplantation in 24 pigs. All animals had haemodynamic disturbances due to the technique of the operation: metabolic acidosis, disturbances of bioelectric activity of the heart and electrolyte level changes. The methods of management of these disturbances are discussed.

Antibody titers by mixed agglutination to varicella-zoster, herpes simplex and vaccinia viruses in patients with multiple sclerosis.

Antibody titers to varicella-zoster (V-Z), herpes simplex (HSV) and vaccinia viruses were determined in sera collected in Buffalo, NY, from patients with multiple sclerosis (MS), patients with other diseases, and normal individuals. The mixed agglutination test employing cell cultures infected with one of the above viruses as a source of antigen was used to determine antibody titers. The geometric mean titer (GMT) for V-Z in 59 MS cases was significantly higher than that observed in patients with other diseases and in normal individuals.

Studies on acute lung lymphatic edema.

The outflow from lymphatic vessels running along with the right cardinal bronchus was interrupted in six dogs by the removal of the corresponding hilar lymph nodes. After 6-8 days the right lung showed slight subpleural areas of atelectasis and edema. Edema of endothelial cells of the lung septal capilaries was observed electron-microscopically.

Cytodiagnosis of acinic cell carcinoma. Ultrastructural study of material obtained by fine needle aspiration biopsy.

The material obtained by fine needle aspiration biopsy from a metastatic acinic cell carcinoma was evaluated by light and electron microscopy. A preliminary diagnosis of acinic cell carcinoma established by light microscopy was confirmed by electron microscopic study. Glandular arrangement of cancerous cells and the presence of a specific type of secretory granules in their cytoplasm were the most characteristic ultrastructural features of the acinic cell carcinoma.