Publications by authors named "Olivier Matthee"
Article Synopsis
- - Canine leishmaniasis, caused by Leishmania protozoa and transmitted by sandflies, is an exotic disease in South Africa, affecting dogs, humans, and wildlife.
- - A risk assessment study aimed to evaluate the probability of this disease entering South Africa through imported dogs, identifying 11 risk factors and analyzing data on diagnostic tests from 44 exporting countries.
- - The study found a moderate risk of Leishmania introduction and suggested risk mitigation strategies, including wildlife surveillance and monitoring of sandfly populations, due to reported cases in neighboring regions.
Quantitative real-time PCR assays previously developed for the detection of Theileria equi and Babesia caballi, were combined in a single multiplex TaqMan qPCR platform for the simultaneous detection of both heamoprotozoan parasites in equids. The multiplex equine piroplasmosis (M-EP) qPCR assay was shown to be efficient and specific. The detection limit was determined to be 1.
View Article and Find Full Text PDFA total of 719 serum samples collected from clinically healthy cattle from eight provinces located in different districts of South Africa were examined by the indirect enzyme-linked immunosorbent assay (ELISA) and the standard indirect fluorescent antibody test (IFAT) to determine the serological prevalence of Babesia bovis and Babesia bigemina. The results showed that 35.3% and 39.
View Article and Find Full Text PDFA competitive enzyme-linked immunosorbent assay (cELISA) based on a broadly conserved, species-specific, B-cell epitope within the C terminus of Babesia bigemina rhoptry-associated protein 1a was validated for international use. Receiver operating characteristic analysis revealed 16% inhibition as the threshold for a negative result, with an associated specificity of 98.3% and sensitivity of 94.
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