Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3.

We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and gene detection sensitivity. In a rigorous comparison with the 10X platform using human primary CD4 + T-cells, HT Smart-seq3 demonstrated higher cell capture efficiency, greater gene detection sensitivity, and lower dropout rates.

Development of Low Frequency (20-100 kHz) Clinically Viable Ultrasound Applicator for Chronic Wound Treatment.

This paper details the systematic approach used to develop a viable clinical prototype of a therapeutic ultrasound applicator and discusses the rationale and deliberations that led to the design strategy. The applicator was specifically devised to treat chronic wounds and-to the best of the author's knowledge-is the first truly wearable device with a proven record of reducing healing time, directly translating to a reduction of healthcare costs. The prototype operates in the kHz (20-100) range of frequencies and uses noncavitational and nonthermal levels of ultrasound energy.

Vascular Endothelial-Breast Epithelial Cell Coculture Model Created from 3D Cell Structures.

Endothelial cell interactions with normal and cancerous breast epithelial cells have been widely studied in tissue growth and development, as well as in angiogenesis and metastasis. Despite the understanding that 3D multicellular architecture is critical to the cell phenotype, 3D vascular structures have not yet been cocultured with 3D breast spheroids . The objective of this study was therefore to create a hierarchical, multiscale model of vascular endothelial-breast epithelial cell interactions in which both cell types were assembled into their 3D architectures.

Endothelial directed collective migration depends on substrate stiffness via localized myosin contractility and cell-matrix interactions.

Macrovascular endothelial injury, which may be caused by percutaneous intervention, requires endothelial cell directed collective migration to restore an intact endothelial monolayer. While interventions are often performed in arteries stiffened by cardiovascular disease, the effect of substrate stiffness on endothelial cell collective migration has not been examined. We studied porcine aortic endothelial cell directed collective migration using a modified cage assay on 4, 14, and 50kPa collagen-coated polyacrylamide gels.

Contribution of Abcc10 (Mrp7) to in vivo paclitaxel resistance as assessed in Abcc10(-/-) mice.

Recently, we reported that the ATP-binding cassette transporter 10 (ABCC10), also known as multidrug resistance protein 7 (MRP7), is able to confer resistance to a variety of anticancer agents, including taxanes. However, the in vivo functions of the pump have not been determined to any extent. In this study, we generated and analyzed Abcc10(-/-) mice to investigate the ability of Abcc10 to function as an endogenous resistance factor.