An Alternative Microbiological Validation for an Online Water Bioburden Analyzer.

Background: The Mettler-Toledo 7000RMS analyzer is a bio-fluorescent particle counter (BFPC) used to monitor real-time bioburden results from purified water (PW).

Objective: Validation of the analyzer using 13 microorganisms and a low-intensity, fluorescent, polystyrene bead.

Methods: During the execution of the validation, a laboratory water system that met PW quality standards was connected to the 7000RMS, and a syringe pump was used to introduce various concentrations of microorganisms and fluorescent polystyrene beads to the analyzer.