Systematic characterization of human prostatic fluid proteins with two-dimensional electrophoresis.

We present a systematic analysis of human prostatic fluid with two-dimensional gel electrophoresis (the ISO-DALT system) and a characterization of normal and disease-related protein patterns. A reference map for prostatic fluid proteins was established by analysis of pooled prostatic fluids from 80 men (age less than or equal to 50 years) without prostatic lesions. Proteins in prostatic fluid that share immunogenicity with serum proteins were identified by use of antibody to whole human-serum protein in an affinity-column fractionation of a reference pool and differential analysis of the absorbed (serum components) and unabsorbed (non-serum components) fractions.

Chest pain in rubber chemical workers exposed to carbon disulphide and methaemoglobin formers.

A cross sectional prevalence study of chest pain in 94 rubber chemical workers exposed to carbon disulphide (CS2) and methaemoglobin forming aromatic amines was carried out. The purpose of the study was to determine whether the prevalence of chest pain or coronary heart disease (CHD), or both, in exposed individuals exceeded that of a group of non-exposed individuals from the same plant. Cardiovascular, smoking, and occupational histories were obtained.

Characterization of a sheep elastin cDNA clone containing translated sequences.

mRNA, isolated from the ligamentum nuchae of fetal sheep by guanidine HCl extraction and oligo(dT) cellulose chromatography, was used to synthesize blunt-ended cDNA molecules by the successive application of AMV reverse transcriptase, DNA polymerase and S1 nuclease. The cDNA was centrifuged on a 15-30% sucrose gradient and molecules greater than 700 bp were tailed with dCTP and cloned into the PstI site of pBR322 which had been tailed with dGTP. Ampicillin-sensitive and tetracycline-resistant colonies were screened by in situ hybridization with elastin-enriched mRNA that had been terminally labeled with 32p.

Haemodynamic effects of a single low dose of prazosin in patients with chronic congestive cardiac failure correlations with pharmacokinetics.

The haemodynamic effects and pharmacokinetics of a single orally administered dose of 0.5 mg of prazosin have been compared in six patients with stable severe congestive cardiac failure. Administration of prazosin induced significant decreases in mean pulmonary capillary wedge pressure (from 27.

Disseminated Mycobacterium chelonei infection following cadaveric renal transplantation: favorable response to cefoxitin.

This article describes a case of disseminated Mycobacterium chelonei infection in a renal transplant recipient. This patient, who underwent thoracic duct drainage prior to cadaveric renal transplantation, developed M chelonei bacteremia and numerous subcutaneous nodules a few weeks after transplantation. The M chelonei initially responded to amikacin and tetracycline.

129I in Missouri thyroids.

Concentrations of 129I and values of the 129I/127I ration are reported in one sample of indigenous vegetation and in over forty additional individual thyroids of man, wild deer and beef cattle in Missouri. The results of this and other studies in our laboratory indicate the following order for successively lower values of 129I/127I ratios in the local environment: Rain, wild deer, commercial milk, beef cattle and human. The value of the 129I/127I ratio in the single vegetation sample is intermediate to the mean values in wild deer and commercial milk, but well within the range of values observed in both.

Evaluation of single-cell classification schemes for computer classification of cervical cells.

Three single-cell classification schemes were evaluated and compared with the performance of a cytotechnologist in classifying single cells from routinely prepared cervical smears. All of the single-cell classification schemes were found to approach the performance of the cytotechnologist in distinguishing normal squamous cells from significantly dysplastic or malignant squamous cells. For distinguishing non-squamous cell types from dysplastic or malignant squamous cells, however, all three schemes fell far short of the cytotechnologist's performance in classifying the same cells.

Effects of captopril (SQ 14,225) in a patient with primary pulmonary hypertension.

In a 33-year-old patient with severe primary pulmonary hypertension, the acute administration of the angiotensin-converting-enzyme inhibitor captopril (SQ 14,225) induced a rise in cardiac output, and a fall in both pulmonary and systemic vascular resistance. Subsequent chronic oral administration of captopril induced only transient clinical improvement, and the patient died. Captopril may nevertheless be useful in the treatment of less advanced cases of this disease.

Use of dopamine and prazosin combined in the treatment of cardiogenic shock.

Seventeen patients who developed cardiogenic shock after acute myocardial infarction were treated with intravenously infused dopamine. In eight of these patients a stable blood pressure was attained, but oliguria or anuria persisted, and oral treatment with prazosin was instituted. Diuresis occurred in seven of these patients, but was followed by transient hypotension associated with a rapid rise in plasma prazosin levels in three.

Detection of Carcinoma of the Prostate Utilizing Biochemical Observations.

This review presents the current status of attempts to identify individuals with a high risk of carcinoma of the prostate by using biochemical, immunochemical, and immunologic studies of body fluids. Blood, urine, and prostatic fluid have been subjected to a variety of analyses in attempts to find alterations that are sufficiently specific and sensitive enough to be useful in the early recognition of patients with a high probability of localized or disseminated carcinoma of the prostate. A variety of immunologic and immunochemical techniques to identify and quantify acid phosphatase of prostatic origin in the serum are currently being explored for this purpose; as yet, none has achieved the specificity-sensitivity relationship necessary for widespread clinical application.

Biochemical profiles of prostatic fluid from normal and diseased prostate glands.

The attempt to identify changes in the biochemical composition of prostatic fluid that might accompany and characterize disease states in the prostate was stimulated by two assumptions based on observations. First, the composition of prostatic fluid was judged to be likely to reflect the metabolic status of at least the epithelial cells accurately. Secondly, the metabolic changes preceding or associated with the development of carcinoma in the prostate seemed likely to be diffuse rather than limited to the histologically abnormal prostatic cells.

Analysis of specific proteins in prostatic fluid for detecting prostatic malignancy.

In an attempt to identify an indicator(s) specifically associated with prostatic cancer prostatic fluid was collected by rectal massage from patients with prostatic cancer, prostatitis, benign prostatic hyperplasia and from those without recognized prostatic lesions in order to measure various immunoproteins. The proteins examined were IgG, IgA, IgM, complements C3 and C4, and transferrin. Prostatic fluid samples were subjected first to immunoelectrophoresis.