-Succinyltransferase Encoded by a Cryptic Siderophore Biosynthesis Gene Cluster in Modifies Structurally Distinct Antibiotics.

The antibiotic desertomycin A and its previously undescribed inactive -succinylated analogue, desertomycin X, were isolated from sp. strain YIM 121038. Genome sequencing and analysis readily identified the desertomycin biosynthetic gene cluster (BGC), which lacked genes encoding acyltransferases that would account for desertomycin X formation.

spp. From the Marine Sponge : Analyses of Secondary Metabolite Biosynthesis Gene Clusters and Some of Their Products.

Actinomycete bacteria from marine environments represent a potential source for new antibiotics and anti-tumor drugs. Ten strains belonging to the genus isolated from the marine sponge collected at the bottom of the Trondheim fjord (Norway) were screened for antibiotic activity. Since only few isolates proved to be bioactive in the conditions tested, we decided to gain an insight into their biosynthetic potential using genome sequencing and analysis.

sp. nov., a novel species of the genus , isolated from alkaline soil.

A Gram-strain positive, mycelium-forming actinomycete, YIM 121212, was isolated from an alkaline soil sample collected in Yunnan province, PR China. Classification using a polyphasic approach indicated that YIM 121212 represents a member of the genus , and is closely related to SCSIO 11529 (99.31 %), SP28S-3 (99.

Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.

For over seven decades, bacteria served as a valuable source of bioactive natural products some of which were eventually developed into drugs to treat infections, cancer and immune system-related diseases. Traditionally, novel compounds produced by bacteria were discovered via conventional bioprospecting based on isolation of potential producers and screening their extracts in a variety of bioassays. Over time, most of the natural products identifiable by this approach were discovered, and the pipeline for new drugs based on bacterially produced metabolites started to run dry.

Genome Mining of sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic.

bacteria are recognized as an important source for antibiotics with broad applications in human medicine and animal health. Here, we report the isolation of a new lichen-associating sp. YIM 130001 from the tropical rainforest in Xishuangbanna (Yunnan, China), which displayed antibacterial activity against .

Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by sp. NP10.

sp. NP10 was previously shown to synthesize large amounts of free fatty acids (FFAs). In this work, we report the first insights into the biosynthesis of these fatty acids (FAs) gained after genome sequencing and identification of the genes involved.

An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts.

Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic.

Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties.

Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B. methanolicus strains MGA3 (ATCC53907) and PB1 (NCIMB13113) were sequenced and found to harbor three different putative Mdh encoding genes, each belonging to the type III Fe-NAD(+)-dependent alcohol dehydrogenases.