Degradation of a Model Mixture of PAHs by Bacterial-Fungal Co-Cultures.

Background: Bacteria and fungi are the most important soil organisms owing to their abundance and the key roles they play in the functioning of ecosystems. We examined possible synergistic and antagonistic effects during the degradation of polycyclic aromatic hydrocarbons (PAHs) by co-cultures of ascomycetes and a plant-growth-promoting bacterium.

Methods: Bacteria and fungi were grown in a liquid nutrient medium supplemented with PAHs.

Natural and Technical Phytoremediation of Oil-Contaminated Soil.

Natural and technical phytoremediation approaches were compared for their efficacy in decontaminating oil-polluted soil. We examined 20 oil-contaminated sites of 800 to 12,000 m each, with different contamination types (fresh or aged) and levels (4.2-27.

Effect of remediation techniques on petroleum removal from and on biological activity of a drought-stressed Kastanozem soil.

Many petroleum extraction and refinement plants are located in arid climates. Therefore, the remediation of petroleum-polluted soils is complicated by the low moisture conditions. We ran a 70-day experiment to test the efficacy of various combining of remediation treatments with sorghum, yellow medick, and biochar to remove petroleum from and change the biological activity of Kastanozem, a soil typical of the dry steppes and semideserts of the temperate zone.

Physiological and biochemical characteristic of Miscanthus × giganteus grown in heavy metal - oil sludge co-contaminated soil.

The effect of oil sludge and zinc, present in soil both separately and as a mixture on the physiological and biochemical parameters of Miscanthus × giganteus plant was examined in a pot experiment. The opposite effect of pollutants on the accumulation of plant biomass was established: in comparison with uncontaminated control the oil sludge increased, and Zn reduced the root and shoot biomass. Oil sludge had an inhibitory effect on the plant photosynthetic apparatus, which intensified in the presence of Zn.

Mycolicibacterium sp. strain PAM1, an alfalfa rhizosphere dweller, catabolizes PAHs and promotes partner-plant growth.

This research was focused on the isolation and characterization of a PAH-catabolizing mycobacterial strain from the petroleum hydrocarbon-contaminated rhizosphere of alfalfa, as well as on revealing some points of interaction between the microorganism and the plant. Mycolicibacterium sp. PAM1, a pyrene degrader isolated from the niche of interest to us, can catabolize fluoranthene, anthracene, fluorene, and phenanthrene.

Study of plants for phytoremediation of oil-contaminated soil.

Long-term field observations of the natural vegetation cover in industrial and adjacent areas has revealed that the was one of the main plant family representatives of which were noted in oil-contaminated area. In this study against the background of the previously well characterized plant families and , the phytoremediation potential of plants was investigated under the field conditions and described. Among the members of this family, , , , , and were the most common in oil-contaminated areas.

Combined effects of cadmium and oil sludge on sorghum: growth, physiology, and contaminant removal.

The physiological and biochemical responses of Sorghum bicolor (L.) Moench. to cadmium (Cd) (30 mg kg) and oil sludge (OS) (16 g kg) present in soil both separately and as a mixture were studied in pot experiments.

Plant-Bacterial Degradation of Polyaromatic Hydrocarbons in the Rhizosphere.

Studies of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria and plant root exudate enzymes from contaminated rhizospheres suggest that pollutant rhizodegradation involves the joint work of bacteria and plants. Plant-microbial associations with coupled metabolic capabilities that completely degrade PAHs while avoiding the formation of dangerous intermediates are biotechnologically promising.

Degradative properties of two newly isolated strains of the ascomycetes Fusarium oxysporum and Lecanicillium aphanocladii.

Two ascomycete strains were isolated from creosote-contaminated railway sleeper wood. By using a polyphasic approach combining morpho-physiological observations of colonies with molecular tools, the strains were identified as Fusarium oxysporum Schltdl. (IBPPM 543, MUT 4558; GenBank accession no.

The degradative activity and adaptation potential of the litter-decomposing fungus Stropharia rugosoannulata.

The ability of the litter-decomposing basidiomycete Stropharia rugosoannulata DSM 11372 to degrade a wide range of structurally different environmental pollutants such as polycyclic aromatic hydrocarbons (PAHs: phenanthrene, anthracene, fluorene, pyrene, and fluoranthene), synthetic anthraquinone dyes containing condensed aromatic rings, environmentally relevant alkylphenol and oxyethylated alkylphenol representatives, and oil was demonstrated within the present study. 9,10-Anthraquinone, phenanthrene-9,10-quinone, and 9-fluorenone were identified as products of anthracene, phenanthrene, and fluorene degradation, respectively. Fungal degradation was accompanied by the production of the ligninolytic enzymes: laccase and Mn peroxidase, suggesting their involvement in pollutant degradation.

The degradation of three-ringed polycyclic aromatic hydrocarbons by wood-inhabiting fungus Pleurotus ostreatus and soil-inhabiting fungus Agaricus bisporus.

The degradation of two isomeric three-ringed polycyclic aromatic hydrocarbons by the white rot fungus Pleurotus ostreatus D1 and the litter-decomposing fungus Agaricus bisporus F-8 was studied. Despite some differences, the degradation of phenanthrene and anthracene followed the same scheme, forming quinone metabolites at the first stage. The further fate of these metabolites was determined by the composition of the ligninolytic enzyme complexes of the fungi.

Dynamics of natural revegetation of hydrocarbon-contaminated soil and remediation potential of indigenous plant species in the steppe zone of the southern Volga Uplands.

The result of monitoring of natural vegetation growing on oil-contaminated (2.0-75.6 g/kg) and uncontaminated (0.

Peroxidases from root exudates of Medicago sativa and Sorghum bicolor: Catalytic properties and involvement in PAH degradation.

Peroxidases from root exudates of sorghum (Sorghum bicolor L. Moench) and alfalfa (Medicago sativa L.) were purified and characterized, and their ability to oxidize native PAHs and PAH-derivatives was evaluated.

Comparison of the phytoremediation potentials of Medicago falcata L. And Medicago sativa L. in aged oil-sludge-contaminated soil.

Thirteen-year monitoring of the vegetation growing in the industrial and adjacent areas of an oil refinery showed the prevalence of yellow medick (Medicago falcata L.) over other plant species, including alfalfa (Medicago sativa L.).

The coupling of the plant and microbial catabolisms of phenanthrene in the rhizosphere of Medicago sativa.

We studied the catabolism of the polycyclic aromatic hydrocarbon phenanthrene by four rhizobacterial strains and the possibility of enzymatic oxidation of this compound and its microbial metabolites by the root exudates of alfalfa (Medicago sativa L.) in order to detect the possible coupling of the plant and microbial metabolisms under the rhizospheric degradation of the organic pollutant. A comparative study of phenanthrene degradation pathways in the PAH-degrading rhizobacteria Ensifer meliloti, Pseudomonas kunmingensis, Rhizobium petrolearium, and Stenotrophomonas sp.

PERN: an EU-Russia initiative for rhizosphere microbial resources.

Millions of microbial taxa inhabit the rhizosphere and could be used as biofertilizers, biopesticides, and/or for bioremediation. Only a fraction of these microbes have been described and/or are being utilized. Most are dispersed in collections, but coordination of their accessibility and availability is challenging.

Effect of cadmium stress and inoculation with a heavy-metal-resistant bacterium on the growth and enzyme activity of Sorghum bicolor.

In this study, the influence of the heavy-metal-resistant rhizobacterial inoculant Rhodococcus ruber N7 on the growth and enzyme activity of Sorghum bicolor (L.) Moench. under cadmium stress was investigated in quartz sand pot experiments.

Degradation of phenanthrene by the rhizobacterium Ensifer meliloti.

The biodegradation of the polycyclic aromatic hydrocarbon phenantherene by the rhizobacterial strain Ensifer meliloti P221, isolated from the root zone of plant grown in PAH-contaminated soil was studied. Bacterial growth and phenanthrene degradation under the influence of root-exuded organic acids were also investigated. Analysis of the metabolites produced by the strain by using thin-layer chromatography, gas chromatography, high-pressure liquid chromatography, and mass-spectrometry revealed that phenanthrene is bioconverted via two parallel pathways.

Diverse effects of arsenic on selected enzyme activities in soil-plant-microbe interactions.

Under the influence of pollutants, enzyme activities in plant-microbe-soil systems undergo changes of great importance in predicting soil-plant-microbe interactions, regulation of metal and nutrient uptake, and, ultimately, improvement of soil health and fertility. We evaluated the influence of As on soil enzyme activities and the effectiveness of five field crops for As phytoextraction. The initial As concentration in soil was 50mg As kg(-1) soil; planted clean soil, unplanted polluted soil, and unplanted clean soil served as controls.

Isolation and characterization of a glyphosate-degrading rhizosphere strain, Enterobacter cloacae K7.

Plant-growth-promoting rhizobacteria exert beneficial effects on plants through their capacity for nitrogen fixation, phytohormone production, phosphate solubilization, and improvement of the water and mineral status of plants. We suggested that these bacteria may also have the potential to express degradative activity toward glyphosate, a commonly used organophosphorus herbicide. In this study, 10 strains resistant to a 10 mM concentration of glyphosate were isolated from the rhizoplane of various plants.

Versatile peroxidase of Bjerkandera fumosa: substrate and inhibitor specificity.

The inhibitor and substrate specificities of versatile peroxidase from Bjerkandera fumosa (VPBF) were studied. Two different effects were found: NaN(3), Tween-80, anthracene, and fluorene decreased the activity of VPBF, but p-aminobenzoic acid increased it. A mixed mechanism of effector influence on the activity of this enzyme was shown.

Rhizosphere indole-3-acetic acid as a mediator in the Sorghum bicolor-phenanthrene-Sinorhizobium meliloti interactions.

We studied a model system consisting of Sorghum bicolor, phenanthrene, and an auxin-producing polycyclic aromatic hydrocarbon-degrading Sinorhizobium meliloti strain to clarify whether rhizosphere indole-3-acetic acid (IAA) takes part in the plant-pollutant-bacteria interactions. Phenanthrene and S. meliloti treatments of sorghum contributed to a decrease in the rhizosphere IAA concentration and to phytohormone accumulation, respectively.

Emulsifying agent production during PAHs degradation by the white rot fungus Pleurotus ostreatus D1.

For the first time the production of an emulsifying agent during phthalic, 2,2'-diphenic and alpha-hydroxy-beta-naphthoic acids, phenanthrene, anthracene, fluorene, pyrene, fluoranthene, and chrysene degradation by white rot fungus Pleurotus ostreatus was found. The emulsifying activity of the cultivation medium after degradation of these compounds was assessed. Maximal activities were found in the presence of chrysene (48.

Oxidoreductase activity of Sorghum root exudates in a phenanthrene-contaminated environment.

The effect of the polycyclic aromatic hydrocarbon (PAH) phenanthrene on the enzymatic activity of root exudates of the phytoremediating plant Sorghum bicolor (L.) Moench was studied. Analysis of sorghum root exudates allowed us to reveal the activities of oxidase, peroxidase, and tyrosinase.

Rhizosphere microflora of plants used for the phytoremediation of bitumen-contaminated soil.

The microbial communities and their degradative potential in rhizospheres of alfalfa (Medicago sativa) and reed (Phragmites australis) and in unplanted soil in response to bitumen contamination of soil were studied in pot experiments. According to the results of fluorescence microscopy, over a period of 27 months, bitumen contamination of soil reduced the total number of microorganisms more significantly (by 75%) in unplanted than in rhizosphere soil (by 42% and 7% for reed and alfalfa, respectively) and had various effects on some important physiological groups of microorganisms such as actinomycetes as well as nitrogen-fixing, nitrifying, denitrifying, ammonifying, phosphate-solubilizing, sulphur-oxidizing, cellulolytic and hydrocarbon-degrading microorganisms. The changes in the physiological structure of the microbial community under bitumen contamination were found to hinge on not merely the presence of plants but also their type.