Publications by authors named "Olga Suska"
CMTR1 (cap methyltransferase 1) catalyses methylation of the first transcribed nucleotide of RNAPII transcripts (N1 2'-O-Me), creating part of the mammalian RNA cap structure. In addition to marking RNA as self, N1 2'-O-Me has ill-defined roles in RNA expression and translation. Here, we investigated the gene specificity of CMTR1 and its impact on RNA expression in embryonic stem cells.
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- The m7G cap is crucial for RNA produced by RNA Polymerase II and is important for gene expression in eukaryotes, but its specific function in mammals was previously unclear.
- Researchers found that the methyltransferase RNMT plays a significant role in T cell activation by regulating the production of mRNA and ribosomes, which are essential for metabolic changes and rapid cell division.
- RNMT's induction during T cell receptor stimulation leads to increased expression of certain mRNAs and snoRNAs, vital for ribosome biogenesis, and its absence results in decreased ribosome production and impaired T cell proliferation.
Oregano ( L.) and thyme ( L.) have long been known for their organoleptic properties.
View Article and Find Full Text PDFDamaged DNA typically imposes stringent controls on eukaryotic cell cycle progression, ensuring faithful transmission of genetic material. Some DNA breaks, and the resulting rearrangements, are advantageous, however. For example, antigenic variation in the parasitic African trypanosome, , relies upon homologous recombination-based rearrangements of telomeric variant surface glycoprotein () genes, triggered by breaks.
View Article and Find Full Text PDFmRNA cap addition occurs early during RNA Pol II-dependent transcription, facilitating pre-mRNA processing and translation. We report that the mammalian mRNA cap methyltransferase, RNMT-RAM, promotes RNA Pol II transcription independent of mRNA capping and translation. In cells, sublethal suppression of RNMT-RAM reduces RNA Pol II occupancy, net mRNA synthesis, and pre-mRNA levels.
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- The mRNA cap plays a crucial role in processing transcripts and initiating translation, with its methylation being important during embryonic stem cell (ESC) differentiation.
- In ESCs, the increased expression of RAM, an mRNA cap methyltransferase, leads to higher levels of methylation, promoting pluripotency-associated gene expression.
- During neural differentiation, RAM is suppressed, leading to reduced pluripotency factor expression and increased neural gene expression, with ERK1/2 activity playing a key role in this regulation through the degradation of RAM.