Forces of interaction of red blood cells and endothelial cells at different concentrations of fibrinogen: Measurements with laser tweezers in vitro.

Blood microrheology depends on the constituents of blood plasma, the interaction between blood cells resulting in red blood cell (RBC) and platelets aggregation, and adhesion of RBC, platelets and leukocytes to vascular endothelium. The main plasma protein molecule -actuator of RBC aggregation is fibrinogen. In this paper the effect of interaction between the endothelium and RBC at different fibrinogen concentrations on the RBC microrheological properties was investigated in vitro.

A mechanism of self-lipid endocytosis mediated by the receptor Mincle.

Cellular lipid uptake (through endocytosis) is a basic physiological process. Dysregulation of this process underlies the pathogenesis of diseases such as atherosclerosis, obesity, diabetes, and cancer. However, to date, only some mechanisms of lipid endocytosis have been discovered.

Novel Cationic Meso-Arylporphyrins and Their Antiviral Activity against HSV-1.

This work is devoted to the search for new antiherpes simplex virus type 1 (HSV-1) drugs among synthetic tetrapyrroles and to an investigation of their antiviral properties under nonphotodynamic conditions. In this study, novel amphiphilic 5,10,15,20-tetrakis(4-(3-pyridyl--propanoyl)oxyphenyl)porphyrin tetrabromide (), 5,10,15,20-tetrakis(4-(6-pyridyl--hexanoyl)oxyphenyl)porphyrin tetrabromide () and known 5,10,15,20-tetrakis(1-methyl-4-pyridinio)porphyrin tetraiodide () were synthesized, and their dark antiviral activity in vitro against HSV-1 was studied. The influence of porphyrin's nanosized delivery vehicles based on Pluronic F127 on anti-HSV-1 activity was estimated.

Topical treatment with the bacterium-derived c-Met agonist InlB/15 accelerates healing in the abrasion wound mouse model.

Studies of factors affecting wound-healing rates are encouraged by a critical need for new treatments to manage an increasing burden of non-healing wounds. The InlB protein produced by the Gram-positive bacterium Listeria monocytogenes is an agonist of the tyrosine kinase receptor c-Met and a functional analog of the hepatocyte growth factor (HGF), which is a mammalian ligand of c-Met. The recombinant InlB protein, which is the c-Met-binding InlB domain (amino acids 31-321), was cloned from the L.

TNF-alpha and gamma-irradiation induced activation of the Salmonella typhimurium reproduction in the organs of infected animals.

The goal of the current work is to determine the role of the TNF-alpha on the activation of the bacterial growth in an in vivo system. In order to reach this goal we studied the dynamics of the reproduction of vegetative forms of Salmonella and the recultivation of non-cultivating forms of Salmonella in infected animals. Experiments have been conducted both on animals that had been injected with exogenous TNF together with bacterial suspension and on animals that had been exposed to gamma-radiation before infection, since it is known that irradiation increases the secretion of TNF.

Herpes simplex type I virus infected human vascular endothelial cells induce the production of anti-viral and proinflammatory factors by peripheral blood leukocytes in vitro.

Viral infection of the vascular wall cellular elements is involved in development of several pathophysiological events, including vasculitis, transplant rejection, and atherosclerosis. Previously, we have shown that cultured human vascular endothelial cells (ECs) may be effectively infected with herpes simplex type I virus (HSV-1), and this cultural model could be a useful tool for the explanation of many aspects of viral disease. In this study, we investigated the effects of conditioned media (CM) of peripheral blood mononuclear cells (PBMCs) on HSV-1 reproduction and cell adhesion molecule expression in cultured ECs.

Herpes Simplex Type I Virus Infection of Cultured Human Vascular Endothelial Cells: Expression of Cell Adhesion Molecules and Induction of Interferon and Cytokine Production by Blood Mononuclear Cells.

The expression of cell adhesion molecules, P- and E-selectins, ICAM-1, and VCAM-1, was studied in cultured human vascular endothelial cells (ECs) infected by herpes simplex type I virus (HSV-1). It was shown that ECs without any signs of the cytopathogenic effect (CPE) expressed on their surface P- and E-selectins as soon as 24 h after infection. No appearance of VCAM-1 or increase in ICAM-1 expression was detected.

The Effect of Smooth Muscle Cells and Leukocytes on HSV-1 Reproduction in Cultured Human Endothelial Cells.

The ability of intima smooth muscle cells (SMCs) and blood leukocytes to control the development of Herpes simplex type 1 virus (HSV-1) infection in human vascular endothelial cells (ECs) was studied under a co-culture conditions. It was shown that cultured ECs supported HSV-1 reproduction followed by cytopathogenic effect (CPE) and accumulation of infectious virus in the cell culture medium. At the multiplicity of infection (MOI) ranging from 1 to 0.