Nanocomplex of Berberine with C Fullerene Is a Potent Suppressor of Lewis Lung Carcinoma Cells Invasion In Vitro and Metastatic Activity In Vivo.

Effective targeting of metastasis is considered the main problem in cancer therapy. The development of herbal alkaloid Berberine (Ber)-based anticancer drugs is limited due to Ber' low effective concentration, poor membrane permeability, and short plasma half-life. To overcome these limitations, we used Ber noncovalently bound to C fullerene (C).

Mode of photoexcited C fullerene involvement in potentiating cisplatin toxicity against drug-resistant L1210 cells.

C fullerene has received great attention as a candidate for biomedical applications. Due to unique structure and properties, C fullerene nanoparticles are supposed to be useful in drug delivery, photodynamic therapy (PDT) of cancer, and reversion of tumor cells' multidrug resistance. The aim of this study was to elucidate the possible molecular mechanisms involved in photoexcited C fullerene-dependent enhancement of cisplatin toxicity against leukemic cells resistant to cisplatin.

C Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells.

A herbal alkaloid Berberine (Ber), used for centuries in Ayurvedic, Chinese, Middle-Eastern, and native American folk medicines, is nowadays proved to function as a safe anticancer agent. Yet, its poor water solubility, stability, and bioavailability hinder clinical application. In this study, we have explored a nanosized carbon nanoparticle-C fullerene (C)-for optimized Ber delivery into leukemic cells.

Synergy of Chemo- and Photodynamic Therapies with C Fullerene-Doxorubicin Nanocomplex.

A nanosized drug complex was explored to improve the efficiency of cancer chemotherapy, complementing it with nanodelivery and photodynamic therapy. For this, nanomolar amounts of a non-covalent nanocomplex of Doxorubicin (Dox) with carbon nanoparticle C fullerene (C) were applied in 1:1 and 2:1 molar ratio, exploiting C both as a drug-carrier and as a photosensitizer. The fluorescence microscopy analysis of human leukemic CCRF-CEM cells, in vitro cancer model, treated with nanocomplexes showed Dox's nuclear and C's extranuclear localization.

Toxicity of C fullerene-cisplatin nanocomplex against Lewis lung carcinoma cells.

Cisplatin (Cis-Pt) is the cytotoxic agent widely used against tumors of various origin, but its therapeutic efficiency is substantially limited by a non-selective effect and high toxicity. Conjugation of Cis-Pt with nanocarriers is thought to be one option to enable drug targeting. The aim of this study was to estimate toxic effects of the nanocomplex formed by noncovalent interaction of C fullerene with Cis-Pt against Lewis lung carcinoma (LLC) cells in comparison with free drug.

Correction to: Complexation with C Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro.

Following publication of the original article [1], the authors flagged that there was unfortunately an error with Fig. 3 of the article.

Complexation with C Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro.

Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.Here, we studied the physicochemical properties and anticancer activity of C fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin.

HPLC-ESI-MS method for C fullerene mitochondrial content quantification.

The presented dataset describes the quantification of carbon nanoparticle C fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography-electro spray ionization-mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C fullerene. Then, human leukemic cells were incubated with C fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction.

C fullerene accumulation in human leukemic cells and perspectives of LED-mediated photodynamic therapy.

Recent progress in nanobiotechnology has attracted interest to a biomedical application of the carbon nanostructure C fullerene since it possesses a unique structure and versatile biological activity. C fullerene potential application in the frame of cancer photodynamic therapy (PDT) relies on rapid development of new light sources as well as on better understanding of the fullerene interaction with cells. The aim of this study was to analyze C fullerene effects on human leukemic cells (CCRF-CEM) in combination with high power single chip light-emitting diodes (LEDs) light irradiation of different wavelengths: ultraviolet (UV, 365 nm), violet (405 nm), green (515 nm) and red (632 nm).

C60 fullerene as synergistic agent in tumor-inhibitory Doxorubicin treatment.

Background: Doxorubicin (Dox) is one of the most potent anticancer drugs, but its successful use is hampered by high toxicity caused mainly by generation of reactive oxygen species. One approach to protect against Dox-dependent chemical insult is combined use of the cytostatic drug with antioxidants. C60 fullerene has a nanostructure with both antioxidant and antitumor potential and may be useful in modulating cell responses to Dox.

Therapeutic reactive oxygen generation.

An increase of the intracellular reactive oxygen species (ROS) concentration leads to the development of oxidative stress and, thus, to the damage of cell components. The cause-and-effect relations between these processes have not been fully established yet. The ability of photo excited supramolecular composites containing fullerenes C60 immobilized at nanosilica particles to generate reactive oxygen species (ROS) in cells of two types (rat thymocytes, and transformed cells of ascite Erlich carcinoma, EAC, and leucosis L1210) is demonstrated.

Catalytic system of the reactive oxygen species on the C60 fullerene basis.

Aim: To study the ability of fullerenes C(60) to catalyse the reactions of generation of reactive oxygen species (ROS) in water solution after photoexcitation and to affect the vitality of tumor cells in vitro.

Methods: The number and vitality of cultured Ehrlich carcinoma cells or rat thymocytes were determined using tripane blue, ROS levels were registered using the methods of electron paramagnetic resonance (EPR) spectroscopy and spin traps, photoirradiation of water solution of fullerenes C(60) with visible light was carried out using mercury lamp.

Results: Irradiation of water solution of fullerenes C(60) (10(-5) M) was accompanied with generation of ROS with the rate of 10 nMol/ml/min.