MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica.

Background: The serological tests using blood stage antigens might be helpful for detecting recent exposure to parasites, and seroepidemiological studies would aid in the elimination of malaria. This work produced recombinant proteins of PvMSP1 variants and evaluated their capacity to detect IgG antibodies in symptomatic patients from Mesoamerica.

Methods: Three variant genes were cloned in the pHL-sec plasmid, expressed in the Expi293F™ eukaryotic system, and the recombinant proteins were purified by affinity chromatography.