Publications by authors named "Olga Ignatovich"
CD40-CD40L interactions play a critical role in regulating immune responses. Blockade of CD40L by Abs, such as the anti-CD40L Ab 5c8, demonstrated positive clinical effects in patients with autoimmune diseases; however, incidents of thromboembolism (TE) precluded further development of these molecules. In this study, we examined the role of the Fc domain interaction with FcγRs in modulating platelet activation and potential for TE.
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- Targeting the CD28-CD80/86 pathway with an anti-CD28 antagonist shows promise for treating autoimmune diseases.
- The study introduces anti-human CD28 receptor antagonist domain antibodies (dAbs) that effectively inhibit T cell activation, showing a strong correlation between receptor occupancy and functional activity.
- In cynomolgus monkeys, these dAbs successfully inhibited T cell responses without causing depletion or excessive cytokine release, indicating their potential for clinical use in autoimmunity.
This protocol describes a method for creation of a highly diverse and functional synthetic phage-displayed repertoire of fully human domain antibodies (dAbs). The repertoire is based on two human frameworks (one VH and one Vκ) that express well in bacteria and are frequently used in human antibodies. To achieve this, we first build dAb libraries, containing full synthetic diversity at key positions in the complementarity-determining regions (CDRs).
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