Structure and gene cluster of the O antigen of Escherichia coli L-19, a candidate for a new O-serogroup.

Escherichia coli L-19 isolated from a healthy individual did not agglutinate with any of 21 polyvalent antisera that cover 174 E. coli O-serogroups. The strain was studied in respect to the O-antigen (O-specific polysaccharide, OPS) structure and genetics.

Structure of the O-polysaccharide of Providencia alcalifaciens O35 containing an N-[(S)-1-carboxyethyl]-L-alanine (alanopine) derivative of 4-amino-4,6-dideoxyglucose.

The O-polysaccharide of Providencia alcalifaciens O35 was studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(13)C HMBC, and NOESY experiments in D2O and, to detect correlations for NH protons, in a 9:1 H2O/D2O mixture. A unique N-(1-carboxyethyl)alanine (alanopine, Alo) derivative of 4-amino-4,6-dideoxyglucose (Qui4N) was identified as the polysaccharide component. Alanopine was isolated by solvolysis of the polysaccharide with triflic acid followed by acid hydrolysis, and its (2S,4S)-configuration was determined by the specific optical rotation.

Structure of the alanopine-containing O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Proteus vulgaris HSC 438 classified into a new Proteus serogroup, O76.

The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of Proteus vulgaris HSC 438, and the following structure was established by chemical methods and one- and two-dimensional (1)H and (13)C NMR spectroscopy: →3)-β-d-Quip4NAlo-(1→3)-α-d-Galp6Ac-(1→6)-α-d-Glcp-(1→3)-α-l-FucpNAc-(1→3)-β-d-GlcpNAc-(1→, where d-Qui4N stands for 4-amino-4,6-dideoxy-d-glucose and Alo for N-((S)-1-carboxyethyl)-l-alanine (alanopine); only about half of the Gal residues are O-acetylated. This structure is unique among the Proteus O-polysaccharides, and therefore it is proposed to classify P. vulgaris HSC 438 into a new Proteus serogroup, O76.

Structures of cell-wall phosphate-containing glycopolymers of Bifidobacterium longum BIM B-476-D.

Glycopolymers with oligosaccharyl phosphate repeats of two types and ribitol and glycerol teichoic acids were isolated from cell wall of Bifidobacterium longum BIM B-476-D by stepwise extraction with 10% CCl3CO2H. The following structures of the glycopolymers were established by sugar analysis, selective cleavage with aq 2% HOAc, dephosphorylation with 48% HF, 2D NMR spectroscopy, and high-resolution ESI MS: [structure: see text]. The ribitol teichoic acid also contains minor D-alanine, whose position was not determined.

Structure of the O-polysaccharide of Azorhizobium caulinodans HAMBI 216; identification of 3-C-methyl-D-rhamnose as a component of bacterial polysaccharides.

The O-polysaccharide was obtained from the lipopolysaccharide of the stem-nodulating nitrogen-fixing bacterium Azorhizobium caulinodans HAMBI 216 and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy. The polysaccharide was found to have a linear pentasaccharide repeating unit containing D-rhamnose and its rarely occurring 2-O-methyl (Rha2OMe) and 3-C-methyl (Rha3CMe) derivatives and having the following structure: →3)-α-D-Rhap2OMe-(1→2)-β-D-Rhap3CMe-(1→3)-α-D-Rhap-(1→2)-β-D-Rhap3CMe-(1→3)-α-D-Rhap-(1→ .

Structural studies of the O-polysaccharide of Pragia fontium 97U124 containing 2-acetamido-2,4,6-trideoxy-4-(D-glyceroyl)amino-D-glucose.

The O-polysaccharide was obtained by acid hydrolysis of the lipopolysaccharide of Pragia fontium 97U124 and studied by sugar analysis and 1D and 2D NMR spectroscopy. A new bacillosamine derivative, 2-acetamido-2,4,6-trideoxy-4-(D-glyceroyl)amino-D-glucose (D-QuiNAc4NAcyl), was identified as a polysaccharide constituent. The following structure of the O-polysaccharide was established →3)-α-L-FucpNAc-(1→3)-α-L-FucpNAc-(1→3)-β-D-QuipNAc4NAcyl-(1→ This structure is closely related to that of the D-QuiNAc4NAc-containing O-polysaccharide of Pseudomonas aurantiaca IMV 31 established earlier (Knirel, Y.

Structure of the O-antigen of Budvicia aquatica 20186, a new bacterial polysaccharide that contains 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-D-xylo-hexose (yersiniose A).

The following structure of the O-specific polysaccharide (O-antigen) of Budvicia aquatica 20186 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-α-L-Rhap-(1→3)-α-D-Galp-(1→2)-α-Yerp-(1→3)-β-D-GalpNAc-(1→ where Yer stands for 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-D-xylo-hexose (yersiniose A).

Structure of the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582: identification of 4-deoxy-D-xylo-hexose as a component of bacterial polysaccharides.

A novel constituent of bacterial polysaccharides, 4-deoxy-D-xylo-hexose (D-4dxylHex), was found in the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582. D-4dxylHex was isolated in the free state by paper chromatography after full acid hydrolysis of the polysaccharide and identified by GLC-mass spectrometry, 1H and 13C NMR spectroscopy, and specific rotation. It occurs as a lateral substituent in ∼40% of the oligosaccharide repeating units, making the polysaccharide devoid of strict regularity.

Structure of the O-polysaccharide of Pragia fontium 27480 containing 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid.

The following structure of the O-polysaccharide of Pragia fontium 27480 was elucidated by sugar analysis, including determination of the absolute configurations of the monosaccharides, and Smith degradation along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-β-d-ManpNAc3NAcA-(1→2)-α-l-Rhap-(1→3)-β-l-Rhap-(1→4)-α-d-GlcpNAc-(1→ where ManNAc3NAcA stands for 2,3-diacetamido-2,3-dideoxymannuronic acid.

Structure of the O-polysaccharide of the lipopolysaccharide of Pragia fontium 97U116.

The O-polysaccharide of Pragia fontium 97U116 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the pentasaccharide-repeating unit was established: -->2)-alpha-d-Galf-(1-->3)-alpha-l-Rhap2Ac(I)-(1-->4)-alpha-d-GlcpNAc(I)-(1-->2)-alpha-l-Rhap(II)-(1-->3)-beta-d-GlcpNAc(II)-(1-->

Structure of the O-polysaccharides of the lipopolysaccharides of Mesorhizobium loti HAMBI 1148 and Mesorhizobium amorphae ATCC 19655 containing two O-methylated monosaccharides.

The O-polysaccharide of Mesorhizobium loti HAMBI 1148 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation, and (1)H and (13)C NMR spectroscopies, including 2D (1)H/(1)H COSY, TOCSY, ROESY, and H-detected (1)H/(13)C HSQC experiments. The O-polysaccharide was found to have a branched hexasaccharide-repeating unit of the following structure: [Formula: see text] where 2-acetamido-2-deoxy-4-O-methyl-D-glucose (D-GlcNAc4Me) and methyl group on 2-substituted D-rhamnose (Me) shown in italics are present in approximately 80% and approximately 40% repeating units, respectively. Similar studies of the O-polysaccharide from Mesorhizobium amorphae ATCC 19655 by sugar analysis and NMR spectroscopy revealed essentially the same structure but a higher content of 3-O-methyl-D-rhamnose ( approximately 70%).