New synthesis of oligosaccharides modelling the M epitope of the O-polysaccharide.

Brucellosis is a dangerous zoonotic disease caused by bacteria of the genus . Diagnosis of brucellosis is based on the detection in animal and human sera of antibodies to the O-polysaccharide of lipopolysaccharide. The currently employed serodiagnosis of brucellosis relies on the use of the O-polysaccharide as a diagnostic antigen.

Russian collection of vaccine strains: annotation, implementation and genomic analysis.

Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from strains 82, 19 and 75/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the strain REV-1 and the strain 19; note that twice as many animals have been immunized with the former vaccine than with the latter vaccine. The disadvantage of using these preparations is the formation of prolonged post-vaccination seropositivity, which is especially pronounced in animals after immunization with vaccines from strain 19 and strain REV-1.

Development and characterization of a modified Komarov's bullet for ballistic delivery of live Brucella abortus strains 82 and 19 to cattle and bison.

In this study a modified Komarov's bullet was developed to remotely deliver live brucellosis vaccines (Brucella abortus 82 and Brucella abortus 19). After modification, the bullet payload could carry the desired dose (10(11)CFU) of vaccine. As the bullet components were toxic to the live bacteria, a special protective coating was developed for the bullet inner surface that maintained vaccine viability.

Designing vaccines against cattle brucellosis.

The article presents the results from a study of two new vaccines against cattle brucellosis from Brucella abortus cultures. One of these is a live dry vaccine prepared from a weakly agglutinogenic strain in the SR form and the second, an inactivated adjuvant vaccine from an non-agglutinogenic strain in the R form. The immunogenic properties of the vaccines were studied in three tests on heifers with infection by a virulent B.

Targeted prevention of brucellosis in cattle, sheep, and goats in the Russian Federation.

The article presents a brief history of the brucellosis prevention in animals in the world and in the Russian Federation. Data are taken from studies on the immunogenic activity and epizootic efficacy of vaccines against brucellosis in animals, which made it possible, in the final analysis, to regard these preparations as highly important for brucellosis prevention. The relationship between the epizootic brucellosis situation in Russia and the employment of specific agents in targeted prevention of brucellosis in cattle, sheep, and goats, and the sequence of their use, are presented briefly, substantiating the feasibility of their use and improvement.