Influence of aluminum and iron chlorides on the parameters of zigzag patterns on films dried from BSA solutions.

The relationships between the structural and aggregational state of bovine serum albumin (BSA) and the specific length and total number of zigzag pattern segments of the film textures formed upon drying biopolymer solutions with aluminum and iron chlorides have been shown. To obtain films, saline solutions of BSA were dried in a glass cuvette under thermostatically controlled conditions. It is shown that the formation of zigzag structures is sensitive to the influence of aluminum chlorides Al and iron chlorides Fe and depend on the concentration of AlCl and FeCl.

Textures on the surface of BSA films with different concentrations of sodium halides and water state in solution.

The formation of the textures on the surface of the films from the solutions of bovine serum albumin (BSA) with sodium halides (NaF, NaCl, and NaBr) of various concentrations was studied. The formation of symmetric zigzag textures on the surface of BSA films (Cryst Eng 3:173-194, 2000) in the presence of sodium halides depends on the conformational state of the protein globule. Thermal denaturation of BSA also did not allow to form zigzag textures on the surface of the films.

Dielectric properties of human ovary follicular fluid at 9.2 GHz.

The influence of the follicle size, rapid freezing to -196 degrees capital ES, Cyrillic and cryopreservation in liquid nitrogen within a period of one month of the human ovary follicular fluid (FF) on its dielectric properties is studied by the microwave dielectric method. The FF was obtained from dominant follicles of patients who received treatment for infertility by extracorporal fertilization. We have measured the real part (epsilon') of the complex permittivity of the native and frozen follicular fluids at the room temperature.

Using UHF-dielectrometry to study protein structural transitions.

UHF-dielectrometry method is based on the following facts: i) there is dispersion (i.e. dependence on frequency) of the dielectric permeability epsilon; ii) bound and free water have remarkable different epsilon, mobility and dispersion regions; iii) conformational changes in a macromolecule lead to redistribution of free and bound water and to change of the amount of free water molecules.