Long-term real-world data of ustekinumab in ulcerative colitis: the Stockholm Ustekinumab Study (STOCUSTE).

Background: Ustekinumab (UST) is an anti-interleukin-12/23 antibody used in the treatment of inflammatory bowel disease. This study includes patients treated at four hospitals in Stockholm to provide long-term real-world data.

Methods: Retrospective study including patients diagnosed with ulcerative colitis and treated with UST between the years 2019 and 2021.

Long-term real-world data of ustekinumab in Crohn's disease: the Stockholm ustekinumab study.

Background: Ustekinumab is used to treat inflammatory bowel disease mainly in patients failing anti-tumour necrosis factor (TNF)-agents.

Objectives: To provide real-world data in unselected patients with Crohn's disease (CD), treated with ustekinumab.

Design: Longitudinal retrospective study at four hospitals in Stockholm, Sweden.

Help-seeking behavior among Danish veterans with self-reported mental problems - a 22 years register-based follow-up study.

To estimate the prevalence of utilization of mental health services (MHS) among Danish veterans with self-reported deployment-related mental problems and to identify predictors for help-seeking behavior for mental problems among veterans. Data on deployment characteristics was obtained from a telephone survey in 2011 among a random sample of veterans deployed during 1996-2009. Only respondents reporting sustained or less sustained mental problems were included, and data from national registers on mental health service utilization and prescribed psychotropics covering up to 22 years of follow-up was obtained.

Bayesian asymmetric regression as a means to estimate and evaluate oral reading fluency slopes.

Bayesian techniques have become increasingly present in the social sciences, fueled by advances in computer speed and the development of user-friendly software. In this paper, we forward the use of Bayesian Asymmetric Regression (BAR) to monitor intervention responsiveness when using Curriculum-Based Measurement (CBM) to assess oral reading fluency (ORF). An overview of Bayesian methods and their application to the problem-solving model is first presented, which is further illustrated by a case example.

High frequency of prostate antigen-directed T cells in cancer patients compared to healthy age-matched individuals.

Background: In order to obtain a sustained cytotoxic T lymphocyte (CTL) response against cancer cells it is preferable to have CTLs directed against multiple peptide epitopes from numerous tumor-associated antigens.

Methods: We used a Flow Cytometry-based interferon (IFN)-gamma secretion assay with peptide-pulsed C1R-A2 as antigen-presenting cells to analyze whether CD8+ T cells directed against any of 24 HLA-A*0201-binding peptides from 15 prostate-associated proteins can be found in the peripheral blood of patients with localized prostate cancer. We also investigated whether multiple prostate antigen-specific CD8+ T cells can be generated simultaneously, from a naïve T cell repertoire.

Strategic use of an adenoviral vector for rapid and efficient ex vivo-generation of cytomegalovirus pp65-reactive cytolytic and helper T cells.

Cytomegalovirus (CMV) reactivation can cause severe complications for transplant patients. Such patients can be protected against CMV-associated diseases through reconstitution of donor-derived CMV-reactive cytolytic and helper T cells. We have developed a strategic protocol for efficient simultaneous generation of CMV-reactive CD8+ and CD4+ T cells ex vivo.

Characterization of human prostate and breast cancer cell lines for experimental T cell-based immunotherapy.

Background: In order to develop experimental immunotherapy for prostate and breast cancer it is of outmost importance to have representative target cell lines that through human leukocyte antigen (HLA) class I molecules present relevant levels of peptides from tumor-associated antigens for cytotoxic T lymphocyte (CTL) recognition.

Methods: We sequenced the HLA-A and HLA-B loci of eight commonly used prostate and breast cancer cell lines and analyzed the surface expression of HLA-ABC, HLA-DR, CD40, CD80, CD86, and CD54 by flow cytometry. We also analyzed the cell lines for mRNA expression from 25 genes reported to be specifically or preferentially expressed by prostate cells.