[IMMUNOCHEMICAL ANALYSIS OF RECOMBINANT CHIMERIC POLYPEPTIDE OspC(gar+afz) OF BORRELIA GARINIIANIP B. AFZELI ISOLATES].

Aim: Comparative study of antigenic properties of recombinant proteins OsPCgar and OsPCafz and recombinant chimeric polypeptide OspCgar+afrz, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia.

Materials And Methods: Recombinant chimeric polypeptide OSpCgar+af, and recombinant mature proteins OSPCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells; purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients.

[Surgical prophylaxis of pulmonary embolism].

144 patients were operated on for deep vein thrombosis. Color duplex lower limb vessels scanning was he basic diagnostic procedure performed preoperatively. Operative treatment corresponded floating trombectomy and main vein placation as a means of pulmonary embolism prophylaxis.

[Invasive treatment of acute coronary syndrome in multiple affection of the coronary arteries].

A comparative assessment of the risk of invasive myocardial revascularizations in patients with multiple affection of coronary arteries and acute coronary syndrome (ACS) without stable ST-elevation on ECG was carried out. Group A (n=45) consisted of patients with one-stage revascularization with coronary bypass (CB) during ACS, group B (n=45) - balloon angioplasty /stenting of symptom-determining artery with subsequent CB in the "cold period" (6 months after ACS episode). It is demonstrated that the one-stage procedure (i.

[Epidemiologic approaches to diagnosis of diseases dependent on ecologic factors].

The article deals with peculiarities in diagnosis of diseases caused by environmental hazards. The diagnosis covers population level and includes evaluation of environmental quality. Analysis of health for whole population and for risk groups enables to reveal "indicator" diseases.

[Analysis of mental functions in patients after coronary bypass surgery].

The neuropsychological examination was carried out in 18 men (study group), mean age 59.7 +/- 6.6 years, before and after aorto-coronary bypass.

[The study of ecology-induced pathologies in regions and settlements (analytical review)].

The authors analyze a problem associated with necessity to single ecologically induced disorders out of general mass of diseases. Diagnosis of ecologically induced disorders necessitates use of ecologic and hygienic monitoring systems designed by the authors, examination combined with observation of environmental changes and health state in population, group and organism.

[Method of determination of ecologically induced actual risk for human health and the degree of stress in medical and ecological situations].

Lipetsk town area was ranked according to maximal or minimal actual risk for public health (with consideration of medical and ecologic situation if influenced by technogenic hazards. That ranking enables to suggest municipal measures aimed at protection of population of ecologic hazards.

[Microalbuminuria as one of the early diagnostic signs of kidney involvement in patients with a purine metabolic disorder].

The examination of 30 patients with purin disbolism (mean age 23.8 years) has demonstrated that microalbuminuria higher than 20 mg/day may serve a diagnostic criterion of early damage to the kidneys. The level of microalbuminuria correlated with the degree of purin metabolism disorder.

[Assessment of the prophylactic effectiveness of an inactivated influenza vaccine by immunizing schoolchildren in the springtime].

To evaluate the prophylactic effectiveness of influenza inactivated chromatographic vaccine, limited epidemiological observations were made on school children aged 11-14 years in Leningrad, in the autumn of 1981 and the spring of 1982. For immunization, made in a single administration, the vaccine composed of A (H3N2) + +A (H1N1) and containing 3.0-3.

[Characteristics of the immunological reactivity of the body in the streptococcal carrier state].

The use of the discrete dynamic method for the treatment of data obtained in the survey of streptococcus carriers has made it possible to find out that their immune status is determined not so much by the quantitative changes in the results of individual immunological tests (for the bactericidal activity of the blood serum, lysozyme, IgG, IgM, IgA and the phagocytic activity of neutrophils), but, to a greater extent, by the interrelations of these characteristics. Significant differences in the interrelations of various humoral characteristics and in their relationship to the phagocytic process have been detected in the group of carriers as compared with the control group.

[Formation and protective functions of antibodies to neuraminidase of the influenza A virus].

The features of production and protective properties of antineuraminidase antibodies in subjects of various ages with influenza infection caused by currently important influenza A virus strains of H1N1 and H3N2 serosubtypes were studied. In children, antineuraminidase antibodies were found to accumulate less intensively than in adults and in infants under 1 year they were detected extremely rarely. In young infants, their titres after the disease decreased more rapidly than antihemagglutinin titres.

[Effect of Streptomyces spheroides proteinases on the mechanism of antibacterial protection in an experiment].

The effect of Streptomyces spheroides proteinases on the process and character of the local reaction to intraperitoneal infection of mice with E. coli was studied experimentally. It was shown that administration of the proteinases promoted a decrease in the dissemination of the abdominal cavity and a more rapid elimination of the microorganisms from the infection foci.

The importance of antineuraminidase antibodies in resistance to influenza A and immunologic memory for their synthesis.

Eight hundred and seventy-seven sera from 360 adults aged 18-50 who were under permanent observation from October 1980 to March 1981 have been studied by haemagglutination-inhibition (HI) and erythrocyte elution-inhibition (EI) tests--a simplified method of antineuraminidase antibody titration. It was demonstrated in some subjects infected with influenza A H1N1 and H3N2 viruses that the antibody rise was to one of the surface antigens only--haemagglutinin or neuraminidase. These subjects made up 5.

[Importance of secretory antibodies in nonsusceptibility to influenza B disease and the prevention of the spread of this virus].

Under the conditions of the natural outbreak of influenza B a rise in the level of antibodies in the blood serum and respiratory tract secretions has been found to occur in nonimmunized persons during this disease. Secretory antibodies, along with serum antibodies and in interaction with them, prevent the development of clinically manifest influenza. The asymptomatic form of influenza infection has been revealed: during this form of the disease the response of the body is limited to the local immunity system.

[Effect of a complex system of controlling influenza on a citywide scale on the etiological structure of acute respiratory diseases].

Materials on the etiological structure of acute respiratory diseases (ARD) and the spread of respiratory virus infection among the population of the city under the conditions of mass vaccinal prophylaxis and chemoprophylaxis are presented. These materials indicate that the proportion of influenza A virus in the etiological structure of ARD decreased by half as the result of introducing the complex system of influenza control in the city. The proportion of other causative agents of ARD before and after introducing the complex system of influenza control irregularly varied among different age groups of the population without statistical significance.

[Antineuraminidase serum antibodies in natural influenza A and immunization with influenza vaccines].

Parallel HI and virus-elution-from-erythrocytes-inhibition (a simplified method for titration of neuraminidase antibody) tests were used for examinations of 1117 blood serum specimens from 440 adults and children under study, 5250 single serum specimens from healthy subjects from birth to 65 years of age, 38 paired serum specimens from children who experienced influenza A/Texas/1/77 disease in the epidemic of 1979-1980, and 590 paired serum specimens from subjects immunized with influenza vaccines. In 7%-23% of influenza patients and immunized subjects antibody rise was observed to only one of the influenza A virus surface antigens, hemagglutinin or neuraminidase. The protective activity of antibody to influenza A virus neuraminidase was as good as that of antihemagglutinins.

[Mechanism of action of an exopolysaccharide from Mycobacterium cyaneum on the local cellular reaction in experimental coli infection].

A study was made of the effect of the exopolysaccharide (PS) M. cyaneum B-646 on cellular reaction in peritoneal exudate of white mice infected with E. coli.

[Emergency prevention of influenza with remantadin].

Prophylactic efficacy of remantadin was studied during influenza epidemics caused by types A (H3N2) and B viruses. More than 15,000 persons were observed. Daily administration of remantadin ensured a decrease in influenza A (but not influenza B) morbidity 1.

[Significance of persons with different blood groups in the influenza type A epidemic process].

To study the susceptibility of persons with different blood groups to influenza A, the presence of infection in a group of young children placed under constant observation for 6 years (1974-1980) and in a group of donors observed during 1979-1980 was studied in different epidemic situations. The susceptibility of the persons under observation to type A influenza viruses was shown to depend both on the blood group of the subjects and on the properties of circulating viruses. Persons with group B (III) blood were more susceptible to the virus at the period when new antigenic variants and serotypes appeared, persons with group O (I) blood were more susceptible to influenza infection at the period of the circulation of virulent strains, while persons with group A (II) blood were more susceptible at the period when less virulent strains circulated.

[Results of a study of collective immunity to influenza A virus (H1N1) from 1976 to 1980].

The time course of the levels of circulating antihemagglutinins to influenza A (H1N1) virus in the populations of the towns in different geographical zones of the USSR was shown to be similar. The intensity of herd immunity in some areas differed considerably, however. The population of older age groups most of whom had no clinically manifest diseases during the influenza A (H1N1) epidemic was shown to experience immunological changes upon exposure to this agent.

[Factors that provoke strophanthin poisoning and its drug prevention].

The effect of various pathogenic factors and drugs on sensitivity to strophanthin and the possibility of pharmacoprophylaxis of toxicosis caused by this cardiotonic agent were studied in acute and chronic experiments on 375 cats. It is shown that impaired blood supply to the myocardium, toxic affection of the liver, sensitization with the cardiac antigen, and the effect of inflammation mediators, mineralocorticoids, and some antianginous agents reduce strophanthin tolerance. The altered sensitivity to strophanthin is successfully corrected with the beta-adrenoblocker alpheprol, hydrocortisone, analgin, dimedrol, and the anesthetic trimecaine.