Localization and antigenicity reduction of immunodominant conformational IgE epitopes on αs1-casein.

αs1-Casein (αs1-CN) is the major allergen in cow milk; however, the understanding of its conformational epitopes remains limited due to the absence of a well-defined three-dimensional structure, which has impeded efforts to effectively reduce its antigenicity. This study employed molecular dynamics simulations (MD), ELISA, cell assays and peptidomes analysis to investigate the critical conformational epitopes of αs1-Casein. MD and immunological analyses identified a dominant conformational epitope encompassing the regions S55-E75 & Y154-T174 & F179-W199, which exhibited strong binding affinity to IgE and triggered the releasing of β-hexosaminidase, histamine and IL-6 in KU812 cells, thereby inducing allergic responses.

New insights into the digestion and bioavailability of a high-melting-temperature solid triacylglycerol fraction in bovine milk fat.

Clarifying the health risks associated with the consumption of high-melting-temperature solid triacylglycerol (TAG) from milk fat has profound significance for the nutritional evaluation and development of new dairy products. Our previous work effectively separated butterfat into solid/liquid fractions (30S and 30L) at 30 °C and successfully reconstituted milk fat globules (MFGs) with these fractions. The current study examined the postprandial digestive and daily metabolic properties of a high-melting-temperature solid TAG fraction by performing animal experiments (rats) with 30S-reconstituted MFG emulsion gavage for 240 min and 30S-containing diet administration for 4 weeks.

Influence of Pasteurization and Storage on Dynamic In Vitro Gastric Digestion of Milk Proteins: Quantitative Insights Based on Peptidomics.

It is important to evaluate the nutritional quality of milk during the shelf-life, especially during home storage, from a consumer viewpoint. In this study, we investigated the impact of pasteurization (85 °C/15 s) and subsequent storage (at 4 °C for 7 days) on the coagulation behavior of milk and protein digestibility in a dynamic in vitro gastric digestion test. A high level of hydration in curd formed in pasteurized milk upon 7-day cold storage compared to raw and pasteurized milk, indicating fast pepsin diffusion in the interior of curds, increasing the hydrolysis rate.

Influence of interfacial adsorption of glyceryl monostearate and proteins on fat crystallization behavior and stability of whipped-frozen emulsions.

The effect of interfacial competitive adsorption of glyceryl monostearate (GMS) with proteins and GMS-fat (anhydrous milk fat; coconut oil) interactions on the fat crystallization behavior and stability of whipped-frozen emulsions were investigated. The results indicated GMS retarded the nucleation of emulsified anhydrous milk fat, but accelerated crystal growth. A contrasting outcome was elicited by emulsified coconut oil.

Structural and functional characteristics of optimised dry-heat-moisture treated cassava flour and starch.

The flexibility of hydrothermal conditions gives rise to an array of effects on the properties of starch-containing systems. This study examined and optimised moisture, temperature and time conditions with regards to functional properties of cassava flour and starch. The derived optimal conditions for cassava flour were 25%, 90 °C and 45 min, respectively, while cassava starch were 27%, 120 °C and 21 min, respectively.

Impact of steam-heat-moisture treatment on structural and functional properties of cassava flour and starch.

In this study, moisture, steam-temperature and time conditions were optimised in relation to functional properties of cassava flour and starch. The optimal conditions for cassava flour were 15% moisture, 120 °C and 1 bar for 10 min and for cassava starch were 19% moisture, 120 °C and 1 bar for 20 min. Structural, physicochemical, viscoelastic and thermal characteristics of cassava flour and starch upon the optimal steam-heat-moisture treatments were examined Volume-based diameter (D) of cassava flour granules reduced (107.