A 4×3 factorial experiment assessed how different dietary protein sources (mixed fishmeal/plant protein vs. plant protein) and lipid levels (10% vs. 20%) affect the growth and nutrient utilization in rainbow trout across three water temperatures (10°C, 14°C, and 18°C) over 180 days.
Results showed that growth factors, including weight gain and feed efficiency, were influenced by the interaction between diet and temperature, particularly highlighting that higher temperatures boosted specific growth rates.
Additionally, mitochondrial enzyme activities in various tissues were significantly affected by diet and temperature, suggesting that shifts in temperature due to climate change might impact fish diets and growth performance.