Poplar Transformation.

First publications of successful Agrobacterium-mediated transformation of tobacco were published more than 30 years ago. Protocols for Agrobacterium-based transformation as well as biolistic bombardment and PEG transformation of protoplasts are available for more than 150 plant species from various plant families. Also for many Populus species and hybrids, adapted transformation protocols have been published.

Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery.

Background: Rapid improvements in the development of new sequencing technologies have led to the availability of genome sequences of more than 300 organisms today. Thanks to bioinformatic analyses, prediction of gene models and protein-coding transcripts has become feasible. Various reverse and forward genetics strategies have been followed to determine the functions of these gene models and regulatory sequences.