Publications by authors named "Olaf Fuchs"

Article Synopsis
  • The study focuses on expanding native chemical ligation (NCL) beyond cysteine by adding a pyridine-containing auxiliary to the peptide N-terminus, enabling access to challenging amino acid junctions.
  • A new auxiliary called MMPyE is introduced, offering greater stability, the ability to ligate at bulky junctions, and can be easily removed under mild basic conditions (pH 8.5).
  • The practicality of this method is illustrated by the successful synthesis of a 120 amino acid peptide with MUC5AC repeats and the creation of a cyclized 21-mer peptide and a His-tagged peptide with RNA polymerase II repeats using the MMPyE auxiliary.
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Ligation auxiliaries are used in chemical protein synthesis to extend the scope of native chemical ligation (NCL) beyond cysteine. However, auxiliary-mediated ligations at sterically demanding junctions have been difficult. Often the thioester intermediate formed in the thiol exchange step of NCL accumulates because the subsequent S→N acyl transfer is extremely slow.

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Aim: To evaluate the different visual pathways represented by the Heidelberg Engineering Perimeter flicker defined form and RareBit (magnocellular and parvocellular respectively) in different age-groups and according to the fatigue.

Methods: Totally 64 eyes of 32 healthy subjects were included in the prospective study. Each participant underwent screening-ophthalmic examination including best-corrected visual acuity, anterior and posterior segment assessment, and visual field examination with Heidelberg Edge Perimetry (HEP)-standard automated perimeter (SAP) 24-2.

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