Ligation auxiliaries are used in chemical protein synthesis to extend the scope of native chemical ligation (NCL) beyond cysteine. However, auxiliary-mediated ligations at sterically demanding junctions have been difficult. Often the thioester intermediate formed in the thiol exchange step of NCL accumulates because the subsequent S→N acyl transfer is extremely slow.
View Article and Find Full Text PDFAim: To evaluate the different visual pathways represented by the Heidelberg Engineering Perimeter flicker defined form and RareBit (magnocellular and parvocellular respectively) in different age-groups and according to the fatigue.
Methods: Totally 64 eyes of 32 healthy subjects were included in the prospective study. Each participant underwent screening-ophthalmic examination including best-corrected visual acuity, anterior and posterior segment assessment, and visual field examination with Heidelberg Edge Perimetry (HEP)-standard automated perimeter (SAP) 24-2.