Publications by authors named "Oksana V Dovzhenko"
Article Synopsis
- RNA interference (RNAi) using short inhibitory RNAs (siRNAs) is a key technique for studying gene functions, particularly in human embryonic stem cells (hESC) and their differentiation.
- Previous work involved stably overexpressing the enhanced green fluorescent protein (EGFP) in hESC, which was then silenced using plasmid vector-based siRNA, resulting in significant reduction of EGFP expression in several derived cell lines.
- Quantitative analysis indicated that the reduction in EGFP was due to RNAi effects rather than changes in genomic DNA, paving the way for future research into the roles of specific genes in hESC processes like self-renewal and differentiation.
The availability of human embryonic stem (HES) cells with a readily evaluated genetic marker such as green fluorescent protein (GFP) could facilitate a number of experimental opportunities. We constructed a novel plasmid with two elongation factor-1alpha (EF-1alpha) promoters (YPL2) to obtain a vector with mammalian promoters for simultaneous transgene expression in HES cells. An enhanced green fluorescent protein (EGFP) cDNA was inserted under the control of the first EF-1alpha promoter to construct plasmid YPL2-EGFP.
View Article and Find Full Text PDFTrophoblast differentiation and early placental development are essential for the establishment of pregnancy, yet these critical events are not readily investigated in human pregnancy. We used embryoid bodies (EBs) prepared from human embryonic stem (hES) cells as an in vitro model of early human development. The levels of human chorionic gonadotropin (hCG), progesterone, and estradiol-17beta in medium from hES cell-derived EBs grown in suspension culture for 1 wk were higher than unconditioned culture medium or medium from undifferentiated hES cells or spontaneously differentiated hES cell colonies.
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