Publications by authors named "Oksana Shtark"

Pea ( L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown.

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Legumes represent an important source of food protein for human nutrition and animal feed. Therefore, sustainable production of legume crops is an issue of global importance. It is well-known that legume-rhizobia symbiosis allows an increase in the productivity and resilience of legume crops.

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Amyloids represent protein aggregates with highly ordered fibrillar structure associated with the development of various disorders in humans and animals and involved in implementation of different vital functions in all three domains of life. In prokaryotes, amyloids perform a wide repertoire of functions mostly attributed to their interactions with other organisms including interspecies interactions within bacterial communities and host-pathogen interactions. Recently, we demonstrated that free-living cells of , a nitrogen-fixing symbiont of legumes, produce RopA and RopB which form amyloid fibrils at cell surface during the stationary growth phase thus connecting amyloid formation and host-symbiont interactions.

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Intensive exchange of nutrients is a crucial part of the complex interaction between a host plant and fungi within arbuscular mycorrhizal (AM) symbiosis. For the first time, the present study demonstrates how inoculation with AMF affects the pea ( L.) root metabolism at key stages of plant development.

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This study focused on the interactions of pea ( L.) plants with phytopathogenic and beneficial fungi. Here, we examined whether the lysin-motif (LysM) receptor-like kinase LYK9 is directly involved in the perception of long- and short-chain chitooligosaccharides (COs) released after hydrolysis of the cell walls of phytopathogenic fungi and identified in arbuscular mycorrhizal (AM) fungal exudates.

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Article Synopsis
  • Alternative splicing (AS) is a process that allows for the creation of different mRNA forms by varying pre-mRNA processing, playing a key role in gene expression regulation.
  • A study was conducted on garden pea roots in symbiosis with arbuscular mycorrhiza, using RNA sequencing to analyze the transcriptome and identify AS profiles, which were found to be largely similar between mycorrhizal and control roots.
  • Eight genes with AS events specific to the mycorrhizal roots were identified, including one related to splicing, suggesting that AS might contribute to the fine-tuning of gene expression during this symbiotic relationship.
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Amyloids are protein aggregates with a highly ordered spatial structure giving them unique physicochemical properties. Different amyloids not only participate in the development of numerous incurable diseases but control vital functions in archaea, bacteria and eukarya. Plants are a poorly studied systematic group in the field of amyloid biology.

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The garden pea ( L.) is a legume crop of immense economic value. Extensive breeding has led to the emergence of numerous pea varieties, of which some are distinguished by accelerated development in various stages of ontogenesis.

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Amyloids represent protein fibrils with a highly ordered spatial structure, which not only cause dozens of incurable human and animal diseases but also play vital biological roles in Archaea, Bacteria, and Eukarya. Despite the fact that association of bacterial amyloids with microbial pathogenesis and infectious diseases is well known, there is a lack of information concerning the amyloids of symbiotic bacteria. In this study, using the previously developed proteomic method for screening and identification of amyloids (PSIA), we identified amyloidogenic proteins in the proteome of the root nodule bacterium Among 54 proteins identified, we selected two proteins, RopA and RopB, which are predicted to have β-barrel structure and are likely to be involved in the control of plant-microbial symbiosis.

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Arbuscular mycorrhiza (AM) is known to be a mutually beneficial plant-fungal symbiosis; however, the effect of mycorrhization is heavily dependent on multiple biotic and abiotic factors. Therefore, for the proper employment of such plant-fungal symbiotic systems in agriculture, a detailed understanding of the molecular basis of the plant developmental response to mycorrhization is needed. The aim of this work was to uncover the physiological and metabolic alterations in pea ( L.

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Article Synopsis
  • Legume crops are a crucial source of food protein, and their productivity can be enhanced through symbiotic relationships with beneficial soil microorganisms like rhizobia and mycorrhizal fungi.
  • Recent research showed that a high-efficiency interaction pea line (K-8274) gained more productivity from these symbionts compared to a low-efficiency line (K-3358).
  • The study identified 111 proteins that differ in expression between the two lines, suggesting that the high-efficiency line benefits from prolonged seed maturation and enhanced cellular processes, highlighting the importance of this trait in breeding programs.
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Large collections of pea symbiotic mutants were accumulated in the 1990s, but the causal genes for a large portion of the mutations are still not identified due to the complexity of the task. We applied a Mapping-by-Sequencing approach including Bulk Segregant Analysis and Massive Analysis of cDNA Ends (MACE-Seq) sequencing technology for genetic mapping the gene of pea which controls the formation of symbioses with both nodule bacteria and arbuscular-mycorrhizal fungi. For mapping we developed an -population from the cross between pea line N24 carrying the mutant allele of and the wild type NGB1238 (=JI0073) line.

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Article Synopsis
  • Lysin-motif receptor-like kinase K1 plays a crucial role in starting symbiosis and maintaining the growth of infection threads in pea plants, suggesting its function is tied to the structure of Nod factors.
  • Studies showed that mutants lacking certain rhizobial factors had less root hair deformation and fewer infection threads, indicating K1's specificity and importance in these processes.
  • Additionally, K1's role seems specific to legume-rhizobial symbiosis as no differences were observed in interactions with arbuscular mycorrhizal fungi, but K1 mutants did show increased sensitivity to aggressive strains, pointing to potential involvement in immune response regulation.
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Plants are able to discriminate and respond to structurally related chitooligosaccharide (CO) signals from pathogenic and symbiotic fungi. In model plants and LysM-receptor like kinases (LysM-RLK) AtCERK1 and OsCERK1 (chitin elicitor receptor kinase 1) were shown to be involved in response to CO signals. Based on phylogenetic analysis, the pea L.

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