Blood serum interleukins levels and their relationship with colon tissue prostanoids in experimental ulcerative colitis.

Objective: Aim: To study IL-1β, IL-6, IL-8, IL-10 blood serum levels and their relationship with colon tissue prostanoids in experimental ulcerative colitis.

Patients And Methods: Materials and Methods: Blood serum interleukins and colon tissue prostanoids were determined by enzyme immunosorbent assay on three groups of WAG population rats (1st control group - rectal saline injection; 2nd control group - 50% ethanol injection; experimental group - injection of 2,4-dinitrobenzene-sulfonic acid in 50% ethanol).

Results: Results: The increased levels of all interleukins both in 2nd control and experimental groups versus 1st control group were found.

Marine Polysaccharides Carrageenans Enhance Eryptosis and Alter Lipid Order of Cell Membranes in Erythrocytes.

Unlabelled: Aim In the current study, hemocompatibility of three major commercially available types of carrageenans (ι, κ and λ) was investigated focusing on eryptosis.

Materials And Methods: Carrageenans of ι-, κ- and λ-types were incubated with washed erythrocytes (hematocrit 0.4%) at 0-1-5-10 g/L for either 24 h or 48 h.

REACTIVE OXYGEN SPECIES GENERATION BY BLOOD LEUCOCYTES OF RATS WITH POLYCYSTIC OVARY SYNDROME UNDER THE CONDITIONS OF INTERMITTENT COLD EXPOSURE.

Objective: The aim of this study was to determine the level of ROS production by blood leukocytes of rats with PCOS under the conditions of intermittent cold exposure.

Patients And Methods: Materials and methods: In the study, 40 immature female rats of the WAG population at the age of 27 days with a body weight of 80-90 g were used. Five groups were formed (8 animals in each group).

THE VIABILITY OF LEUKOCYTES AND REACTIVE OXYGEN SPECIES GENERATION BY THEM IN RATS WITH CHRONIC COLITIS.

Objective: The aim: To assess reactive oxygen species production by leukocytes and their viability in rats with chronic colitis.

Patients And Methods: Materials and methods: Reactive oxygen species production was estimated in leukocytes, isolated from rats with Dextran Sulfate Sodium-induced chronic colitis and control rats, by flow cytometry using the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate. Leukocyte viability and apoptosis stages were assessed by flow cytometry using annexin V and 7-aminoactinomycin D staining.

Experimental Evaluation of Food-Grade Semi-Refined Carrageenan Toxicity.

The safety of food additives E407 and E407a has raised concerns in the scientific community. Thus, this study aims to assess the local and systemic toxic effects of the common food additive E407a in rats orally exposed to it for two weeks. Complex evaluations of the effects of semi-refined carrageenan (E407a) on rats upon oral exposure were performed.

UV Light-Activated GdYVO:Eu Nanoparticles Induce Reactive Oxygen Species Generation in Leukocytes Without Affecting Erythrocytes In Vitro.

Nanoparticles (NPs) have been reported to be promising enhancement agents for radiation therapy. The aim of the study was to assess the cytotoxicity of UV non-treated and UV pretreated GdYVO:Eu nanoparticles against erythrocytes and leukocytes by detecting eryptosis and reactive oxygen species (ROS) generation. Levels of intracellular ROS in erythrocytes and leukocytes using a ROS-sensitive dye 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA), as well as eryptosis rate utilizing annexin V staining, following direct exposure to UV-activated and nonactivated NPs were detected by flow cytometry.

Semi-refined carrageenan promotes generation of reactive oxygen species in leukocytes of rats upon oral exposure but not in vitro.

Aim: To assess the ability of the common food additive E407a (semi-refined carrageenan) to enter leukocytes in vitro and generate reactive oxygen species (ROS) in leukocytes as a whole and granulocytes in particular, both during incubation and in experimental animals.

Methods: ROS production was assessed in leukocytes incubated with E407a for 2 h at the final concentrations of 5 and 10 g/L using the dye 2',7'-dichlorodihydrofluorescein diacetate (HDCFDA), as well as in cells isolated from rats orally exposed to E407a (140 mg/kg of weight) during 2 weeks (n = 8) and control rats (n = 8), by flow cytometry. Carrageenan uptake by leukocytes was estimated by confocal microscopy using incubation of rhodamine B isothiocyanate-labelled carrageenan with leukocyte suspensions.

Effects of semi-refined carrageenan (food additive E407a) on cell membranes of leukocytes assessed in vivo and in vitro.

Aim To assess the impact of semi-refined carrageenan (E407a) on hydrophobic regions of phosphololipid bilayer in cell membranes of leukocytes collected from rats orally administered this food additive and white blood cells incubated with E407a. Methods Fluorescent probes (ortho-hydroxy derivatives of 2,5-diaryl-1,3-oxazole) were used to estimate the state of lipid bilayer in leukocytes obtained from rats orally exposed to the food additive E407a and in white blood cells incubated with E407a. Results No noticeable changes in the physico-chemical properties were observed in the lipid membranes of leukocytes in the region where the probes locate in response to oral intake of semi-refined carrageenan.

Experimental Evaluation of the Impact of Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles on the Carrageenan-Induced Intestinal Inflammation.

Aim: To evaluate the effects of orally administered gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) on the course of chronic carrageenan-induced intestinal inflammation.

Methods: Samples of small intestinal tissue were collected from four groups of rats (intact, after administration of VNPs, with carrageenaninduced intestinal inflammation, with carrageenan-induced intestinal inflammation orally exposed to VNPs) to assess the intestinal morphology and HSP90α expression. Levels of seromucoid, C-reactive protein, TNF-α, IL-1β and IL-10 were determined in blood serum.

A study of enterocyte membranes during activation of apoptotic processes in chronic carrageenan-induced gastroenterocolitis.

Aim To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by the activation of apoptotic processes. Methods Steady-state fluorescence spectroscopy: a study by fluorescent probes - by ortho-hydroxy derivatives of 2,5-diaryl-1,3- oxazole. Activity of apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polymerase in small intestinal homogenates, blood serum levels of matrix metalloproteinase-2 and caspase-3 and the level of DNA fragmentation in small intestinal homogenates were determined.