Publications by authors named "Okhrimenko I"
Article Synopsis
- Ferritin-based hybrids are large macromolecules with potential uses in drug delivery and vaccines, but their design is complicated.
- Researchers designed hybrids using ferritin from Helicobacter pylori and a Small Ubiquitin-like Modifier, discovering that their assembly was influenced by varying preparation methods.
- They developed a quantitative model to analyze how different stoichiometries affected the structure of these hybrids, revealing unexpected patterns and establishing a framework for better design of ferritin-based systems.
One of the promising drug delivery tools is ferritin, which features high stability at a wide range of conditions and protects cargo by its spherical protein shell. We studied the self-assembly into homoglobules of ferritin from H. pylori and a chimeric protein ferritin-SUMO.
View Article and Find Full Text PDFObjective: Aim: To study the psychological peculiarities of the manifestation of leadership qualities by modern managers of police organizations..
Patients And Methods: Materials and Methods: The research conducted in 2022-2024 involved 64 mid-level managers of police organizations and 462 officers from police units directly subordinated to these managers.
Objective: Aim: The aim is to study the main factors of youth's Internet dependence formation.
Patients And Methods: Materials and Methods: We conducted psychodiagnostic examination of students in the National Academy of Internal Affairs. The research involved 154 students: full-time students aged 18-25 (n = 78) and part-time students aged 26-35 (n = 76).
Objective: Aim: To investigate the levels and indicators of professional deformation in instructor-officers with different service experience as a negative factor in their professional health.
Patients And Methods: Materials and Methods: The research, which was conducted in 2022-2023, involved 136 instructor-officers of the National Academy of Internal Affairs aged 25-55 years with different service experience (up to 5 years - 21 people, 5-10 years - 45 people, 10-15 years - 42 people, over 15 years - 28 people).
Results: Results: The dynamics of the levels of professional deformation in instructor-officers of higher educational institutions depending on the experience of their service activities have been determined, and the peculiarities of the manifestation of key professional and psychological indicators that affect their professional health in the course of their service activities have been revealed.
Objective: Aim: To study the attitude of law enforcement officers to motor activity and investigate its impact on their health.
Patients And Methods: Materials and Methods: The research, which was conducted in 2022-2024, involved 163 law enforcement officers under the age of 30. Research methods: bibliosemantic, questionnaire, testing, medical and biological methods, methods of mathematical statistics.
Objective: Aim: To substantiate the methodology for assessing the biological age of adolescent high schoolers (boys and girls).
Patients And Methods: Materials and Methods: Boys aged 14-15 (n = 102) and girls aged 12-13 (n = 101) were studied. The choice of these age groups is explained by the presence of the most significant individual differences in morphological and functional characteristics in these adolescents.
Objective: Aim: To study the study the impact of negative factors of professional activities on the health of law enforcement officers.
Patients And Methods: Materials and Methods: The research involved 89 law enforcement officers (58 men, and 31 women) who served for a year in practical police units after graduating from a higher educational institution. Methods: analysis and generalization of scientific and methodological literature, anthropometry, physiometry, testing, and statistical methods.
Objective: Aim: The aim is to study the motivation of students towards Internet dependent behavior and develop practical recommendations for improving a set of measures for its prevention.
Patients And Methods: Materials and Methods: The research involved 154 students of the National Academy of Internal Affairs. Research methods: analysis and generalization of literature sources, questionnaire, statistical methods.
Objective: Aim: To investigate changes in the indicators of somatic and mental health of instructor-officers in higher educational institutions with specific learning environment under stress.
Patients And Methods: Materials and Methods: The research involved instructor-officers of the National Academy of Internal Affairs (Ukraine, n = 86) with different teaching experiences: up to 10 years (group 1, n = 22), from 10 to 20 years (group 2, n = 31), over 20 years (group 3, n = 33). The study of somatic and mental health was carried out in September-October 2021 (Stage 1) and in September-October 2023 (Stage 2).
Objective: Aim: To investigate the impact of negative factors of professional activities in the context of the COVID-19 pandemic and martial law in Ukraine on the somatic and mental health of instructor-officers with different teaching experiences.
Patients And Methods: Materials and Methods: The research involved 131 instructor-officers with different teaching experiences. Three groups of instructor-officers were formed: Group 1 - instructors with up to 10 years of teaching experience (n = 30), Group 2 - instructors with 10 to 20 years of experience (n = 47), Group 3 - instructors with more than 20 years of experience (n = 54).
Article Synopsis
- Actin exists in three forms: monomeric globular (G-actin), polymeric fibrillar (F-actin), and an inactivated form called I-actin, which forms without cellular folding machinery.
- The transformation from G-actin to I-actin can occur through various methods, including removal of divalent ions, mild denaturants, or heat, and I-actin can aggregate into oligomers of about 14-16 G-actin monomers.
- This study uses small-angle X-ray scattering to investigate the oligomerization process of I-actin, distinguishing it from F-actin and revealing a unique pathway of oligomer formation.
Methionine gamma lyase fused with S3 domain VGF forms octamers and adheres to tumor cells via binding to EGFR.
Biochem Biophys Res Commun
January 2024
Methods for targeting enzymes exhibiting anticancer properties, such as methionine γ-lyase (MGL), have not yet been sufficiently developed. Here, we present the data describing the physico-chemical properties and cytotoxic effect of fusion protein MGL-S3 - MGL from Clostridium sporogenes translationally fused to S3 domain of the viral growth factor of smallpox. MGL-S3 has methioninase activity comparable to native MGL.
View Article and Find Full Text PDFObjective: Aim: To investigate cadets' motivational priorities for physical exercises and sports in the process of their training and the dynamics of their health indicators.
Patients And Methods: Materials and methods: The research was conducted at the National Academy of Internal Affairs in 2018-2022. The research of motivational priorities involved 372 male cadets from the 1st to the 4th instructional years aged 17 to 22.
Objective: The aim: To investigate the impact of special physical training sessions on the formation of cadets' psychological resilience and physical readiness for the stress factors of future professional and combat activities.
Patients And Methods: Materials and methods: The research involved 96 cadets (men) in the 2nd training year of S. P.
Objective: The aim: To investigate the impact of professional stress on the mental health of law enforcement officers.
Patients And Methods: Materials and methods: The research involved two groups of law enforcement officers (aged 25-40): the first group with up to 7 years of service activities (n = 52), the second group - 8-15 years (n = 48). Research methods: bibliosemantic, questionnaire, testing, method of system analysis and generalization, mathematical and statistical.
F-type ATP synthases play a key role in oxidative and photophosphorylation processes generating adenosine triphosphate (ATP) for most biochemical reactions in living organisms. In contrast to the mitochondrial FF-ATP synthases, those of chloroplasts are known to be mostly monomers with approx. 15% fraction of oligomers interacting presumably non-specifically in a thylakoid membrane.
View Article and Find Full Text PDFProteorhodopsins (PRs), bacterial light-driven outward proton pumps comprise the first discovered and largest family of rhodopsins, they play a significant role in life on the Earth. A big remaining mystery was that up-to-date there was no described bacterial rhodopsins pumping protons at acidic pH despite the fact that bacteria live in different pH environment. Here we describe conceptually new bacterial rhodopsins which are operating as outward proton pumps at acidic pH.
View Article and Find Full Text PDFObjective: The aim: To research the morphofunctional status of instructors of higher educational institutions of di"erent age groups in the process of their pedagogical activities.
Patients And Methods: Materials and methods: The research was conducted in the period from 2019 to 2021. The research involved 126 instructor offcers (men) of different age groups: under 30 years of age - 21 people, 31-35 years of age - 27 people, 36-40 years of age - 32 people, 41-45 years of age - 27 people, over 45 years of age - 19 people.
Objective: The aim: To investigate the e!ect of physical training sessions on health and psycho-emotional state of managers of law enforcement agencies.
Patients And Methods: Materials and methods: The research was conducted in 2019-2021. The research involved 155 managers of law enforcement agencies (men) of different age groups.
Background: With age, there is a tendency for the health of law enforcement officers to deteriorate. This especially applies to instructor officers, whose professional activity is characterized by a significant decrease in the volume of motor activity, overloading of the intellectual sphere, and high neuro-emotional stress.
Objective: The aim of the research is to investigate the influence of recreational and healthpromoting training sessions with physical exercise on the health, physical development, and functional status of instructor officers of older age groups.
Objective: Aim: is to study the level and dynamics of the development of physical and psychological qualities of the cadets in the process of engagement in pentathlon classes.
Patients And Methods: Materials and methods: The research involved 18-23 years old cadets (men) of the first-fourth years of training. The level of the cadets' physical qualities was assessed by their results in the 100 m run, pull-ups, 3 km run, overcoming of the obstacle course.
Objective: Aim: The aim is to study and to improve the motivation of mental health preservation of specialists in the field of special and inclusive education according to European experiences.
Patients And Methods: Materials and methods: The experimental part of the research involved the use of the valid psychodiagnostic methods and tech¬niques: direct and indirect observation, standardized questionnaire survey, semi-standardized individual interviews, psychodiag¬nostic methods. The research was attended by 131 Master's degree students (aged 25-27), specialty 053 Psychology, Educational program - special, clinical psychology.
Article Synopsis
- Ferredoxins are small proteins that help with important processes in living things, but a specific type called 3Fe-4S ferredoxins hasn't been studied much because they need special conditions to exist.
- Scientists figured out the structure of two types of these ferredoxins, showing how they work with another protein called CYP143.
- The study found that when FdxE connects to CYP143, it changes its shape a bit, which helps in making different kinds of important chemicals.
Objective: The aim: To find out the impact of exercise on promotion of health and restoration of mental capacity of instructors of higher educational institutions with specific learning environment.
Patients And Methods: Materials and methods: The research was conducted in 2018-2021. Research methods: theoretical analysis of literature sources, documentary method, pedagogical obser¬vation.