Evaluation of the tissue thickness of the supraspinatus and biceps long head tendons using ultrasound among elderly patients with unilateral adhesive capsulitis in the freezing phase.

[Purpose] This study aimed to clarify the changes in the tissue thickness of the abnormal supraspinatus and biceps long-head tendons among elderly patients to select the treatment targets and evaluate the treatment effects in the freezing phase of adhesive capsulitis. [Participants and Methods] Thirty-two elderly patients with unilateral shoulder pain underwent ultrasound of the supraspinatus and biceps long-head tendons, pain evaluation, and orthopedic testing. Both the supraspinatus and biceps long-head tendons were classified as normal, abnormal, and other groups.

Effect of Subsidies and Tax Deductions on Promoting the Construction of Long-Life Quality Houses in Japan.

Securing a quantity of houses for citizens has been the priority of housing policies in Japan. However, these policies shifted from quantity to quality in the 21st century, including the promotion of "long-life quality housing (LLQH)", which contributes to a sustainable and healthy society for the residential sector. Since then, various policies have been introduced at the national and prefectural (local) levels to promote the construction of LLQH.

Cloning and Aggregation Characterization of Rubber Elongation Factor and Small Rubber Particle Protein from Ficus carica.

Rubber elongation factor (REF) and small rubber particle protein (SRPP) are major latex proteins harvested from Hevea brasiliensis (the rubber tree; HbREF and HbSRPP, respectively). Their amino acid sequences exhibit high homology with each other. In the present study, we cloned two cDNAs encoding REF/SRPP-family proteins (FcREF/SRPP-1 and -2) from the laticifers of Ficus carica (fig tree).

Comparison of the pain-relieving effects of transcutaneous electrical nerve stimulation applied at the same dermatome levels as the site of pain in the wrist joint.

[Purpose] The purpose of this study was to develop a proposal for an effective interventional option for therapeutic stimulation sites by comparing the pain-relieving effect of transcutaneous electrical nerve stimulation (TENS) applied to the same dermatome level of the contralateral sites of the dorsal wrist joint with the pain or the neck, or both sites simultaneously. [Subjects and Methods] A control was first established by triggering pain in the left dorsal wrist joints of adult females by using heat stimulation. Three interventions were then performed, comprising the TENS to the contralateral wrist joint (CW) and to the neck (N) at the same dermatome level as the site of pain, and the TENS to both CW and N simultaneously (CWN).

Role of Cys73 in the thermostability of farnesyl diphosphate synthase from Geobacillus stearothermophilus.

Farnesyl diphosphate synthase (FPPase) is an enzyme that catalyzes the condensation between one molecule of dimethylallyl diphosphate (DMAPP) and two molecules of isopentenyl diphosphate (IPP) to produce farnesyl diphosphate (FPP). FPP is an important precursor in the isoprenoid synthesis pathway. In this study, the crystal structure of FPPase from Geobacillus stearothermophilus (GsFPPase) was determined at 2.

Relative and absolute reliability of ultrasound measurements for the thickness of the soft tissue around the shoulder joint of young normal subjects.

[Purpose] The purpose of this study was to examine the reliability of ultrasound measurements by analyzing the relative reliability and absolute reliability when measuring soft tissue thickness around the shoulder joint. [Subjects and Methods] Eleven healthy young adults (22 shoulders) participated in this study. Thickness of the supraspinatus tendon, subacromial bursa, and biceps tendon was measured on both shoulders.

Synthesis of water-dispersible silver nanoparticles by thermal decomposition of water-soluble silver oxalate precursors.

Silver oxalate, one of the coordination polymer crystals, is a promising synthetic precursor for transformation into Ag nanoparticles without any reducing chemicals via thermal decomposition of the oxalate ions. However, its insoluble nature in solvents has been a great disadvantage, especially for systematic control of crystal growth of the Ag nanoparticles, while such control of inorganic nanoparticles has been generally performed using soluble precursors in homogeneous solutions. In this paper, we document our discovery of water-soluble species from the reaction between the insoluble silver oxalate and N,N-dimethyl-1,3-diaminopropane.

Influences of reconstruction and attenuation correction in brain SPECT images obtained by the hybrid SPECT/CT device: evaluation with a 3-dimensional brain phantom.

Objectives: The aim of this study was to evaluate the influences of reconstruction and attenuation correction on the differences in the radioactivity distributions in (123)I brain SPECT obtained by the hybrid SPECT/CT device.

Methods: We used the 3-dimensional (3D) brain phantom, which imitates the precise structure of gray matter, white matter and bone regions. It was filled with (123)I solution (20.

Purification, crystallization and preliminary X-ray analysis of IMP-18, a class B carbapenemase from Pseudomonas aeruginosa.

Class B β-lactamases are known as metallo-β-lactamases (MBLs) and they hydrolyze most β-lactams, including carbapenems. IMP-18, an MBL cloned from Pseudomonas aeruginosa, was overexpressed, purified and crystallized by vapour diffusion for X-ray crystallographic analysis. Preliminary X-ray analysis showed that the crystal diffracted to 2.

Benefits of point-spread function and time of flight for PET/CT image quality in relation to the body mass index and injected dose.

Unlabelled: The PET image quality of overweight patients and patients who receive low injected doses deteriorates because of increases in statistical noise. The purpose of this study was to investigate the benefits of the point-spread function (PSF) and time-of-flight (TOF) for PET/CT image quality in such patients.

Methods: The PET images were reconstructed using the baseline ordered-subsets expectation-maximization algorithm (OSEM), OSEM + PSF, OSEM + TOF, and OSEM + PSF + TOF.

Improvement in PET/CT image quality with a combination of point-spread function and time-of-flight in relation to reconstruction parameters.

Unlabelled: The aim of this study was to investigate the effects of the point-spread function (PSF) and time-of-flight (TOF) on improving (18)F-FDG PET/CT images in relation to reconstruction parameters and noise-equivalent counts (NEC).

Methods: This study consisted of a phantom study and a retrospective analysis of 39 consecutive patients who underwent clinical (18)F-FDG PET/CT. The body phantom of the National Electrical Manufacturers Association and International Electrotechnical Commission with a 10-mm-diameter sphere was filled with an (18)F-FDG solution with a 4:1 radioactivity ratio compared with the background.

Determination of the absolute stereochemistry of limonene and alpha-santalol by Raman optical activity spectroscopy.

Determining the absolute stereochemistry of organic compounds in solution remains a challenge. We investigated the use of Raman optical activity (ROA) spectroscopy to address this problem. The absolute configurations of (+)-(R)- and (-)-(S)-limonene were determined by ROA spectroscopy, which can be applied to smaller amounts of sample as compared with vibrational circular dichroism (VCD) spectroscopy.

Evaluation of the maximum depth of intraluminal appendiceal fluid to diagnose appendicitis with a 64-detector row CT scanner.

Objective: The objective of this study was to investigate whether the criterion "maximum depth of intraluminal appendiceal fluid greater than 2.6 mm" ("DEPTH >2.6 mm"), with the use of 64-detector row computed tomography, is useful to diagnose appendicitis.

Determination of the optimal acquisition protocol of breath-hold PET/CT for the diagnosis of thoracic lesions.

Objective: The aim of this study was to determine the optimal acquisition scan protocol for deep inspiration breath-hold (BH) fluoro-2-deoxy-D-glucose positron emission tomography (PET) for the examination of thoracic lesions.

Methods: We studied 32 thoracic lesions in 21 patients. Whole-body PET/computed tomography (CT) scanning with free breathing (FB) was performed for 3 min per bed position, followed by a BH-CT and five BH-PET for 20 s each.

Phantom study on three-dimensional target volume delineation by PET/CT-based auto-contouring.

Objective: The aim of this study was to determine an appropriate threshold value for delineation of the target volume in PET/CT and to investigate whether we could delineate a target volume by phantom studies.

Methods: A phantom consisted of six spheres (phi 10-37 mm) filled with 18F solution. Data acquisition was performed PET/CT in non-motion and motion status with high 18F solution and in non-motion status with low 18F solution.

Computed tomography criterion for the diagnosis of appendicitis without periappendiceal inflammation in children using the maximum depth of intraluminal appendiceal fluid.

Objective: The objective of this study was to evaluate whether maximum depth of intraluminal appendiceal fluid (DEPTH) is useful in differentiating appendicitis without periappendiceal inflammation from enlarged normal appendices in children.

Methods: We retrospectively evaluated 826 intravenously enhanced abdominal-pelvic computed tomographic examinations in children (aged 0-18 years) using the following criteria for appendicitis: (1) appendiceal wall thickness greater than 3 mm, (2) appendiceal wall enhancement, (3) focal cecal wall thickening, (4) adjacent adenopathy, (5) appendicolith, and (6) DEPTH. Of 826, 192 were classified into the noncomplicated-normal appendix group (85 enlarged normal appendices [diameter >6 mm] without adjacent lesions), the complicated-normal appendix group (44 enlarged normal appendices with adjacent lesions), or the our-appendicitis group (63 operatively proved appendicitis without periappendiceal inflammation).

A simple table lookup method for PET/CT partial volume correction using a point-spread function in diagnosing lymph node metastasis.

Objective: We evaluated the partial volume effect in PET/CT images and developed a simple correction method to address this problem.

Methods: Six spheres and the background in the phantom were filled with F-18 and we thus obtained 4 different sphere-to-background (SB) ratios. Thirty-nine cervical lymph nodes in 7 patients with papillary thyroid carcinoma (15 malignant and 24 benign) were also examined as a preliminary clinical study.

Importance of gated CT acquisition for the quantitative improvement of the gated PET/CT in moving phantom.

Objective: The aim of this study was to investigate the utility of gated PET/CT and CT attenuation correction (AC) for the quantitation of radioactivity.

Methods: An ellipse phantom containing six spheres, ranging from 10 to 37 mm in diameter, was filled with 36.7 kBq/mL of F-18.

Diagnostic performance of Tc-99m HMPAO SPECT for early and late onset Alzheimer's disease: a clinical evaluation of linearization correction.

Objective: This study examined the influence of linearization correction (LC) on brain perfusion single-photon emission computed tomography (SPECT) for the diagnosis of Alzheimer's disease (AD).

Methods: The early onset group (<65 years old) consisted of 10 patients with AD, and the late onset group (>/=65 years old) of 13 patients with AD. Age-matched controls included seven younger and seven older normal volunteers.

Prospective examination of patients suspected of having appendicitis using new computed tomography criteria including "maximum depth of intraluminal appendiceal fluid greater than 2.6 mm".

Objective: The objective of this study was to perform prospective computed tomography (CT) examination of patients suspected of having appendicitis to determine whether our criteria (which include the new criterion "maximum depth of intraluminal appendiceal fluid greater than 2.6 mm") are useful for improving sensitivity and/or specificity in comparison with conventional major criteria.

Methods: Two hundred eighty consecutive patients older than 15 years old and suspected of having appendicitis were examined using CT.

Kinetic studies of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase reaction using 3-desmethyl allylic substrate analogs.

In order to investigate the substrate binding feature of undecaprenyl diphosphate synthase from Micrococcus luteus B-P 26 with respect to farnesyl diphosphate and a reaction intermediate, (Z,E,E)-geranylgeranyl diphosphate, we examined the reactivity of artificial substrate analogs, 3-desmethyl farnesyl diphosphate and 3-desmethyl Z-geranylgeranyl diphosphate, which lack the methyl group at the 3-position of farnesyl diphosphate and Z-geranylgeranyl diphosphate, respectively. Undecaprenyl diphosphate synthase did not accept either of the 3-desmethyl analogs as the allylic substrate, indicating that the methyl group at the 3-position of the allylic substrate is important in the undecaprenyl diphosphate synthase reaction. These analogs showed different inhibition patterns in the cis-prenyl chain elongation reaction with respect to the reactions of farnesyl diphosphate and Z-geranylgeranyl diphosphate as allylic substrate.

Substrate specificities of wild and mutated farnesyl diphosphate synthases from Bacillus stearothermophilus with artificial substrates.

To determine the substrate specificities of wild and mutated types of farnesyl diphosphate (FPP) synthases from Bacillus stearothermophilus, we examined the reactivities of 8-hydroxygeranyl diphosphate (HOGPP) and 8-methoxygeranyl diphosphate (CH(3)OGPP) as allylic substrate homologs. The wild-type FPP synthase reaction of HOGPP (and CH(3)OGPP) with isopentenyl diphosphate (IPP) gave hydroxyfarnesyl- (and methoxyfarnesyl-) diphosphates that stopped at the first stage of condensation. On the other hand, with mutated type FPP synthase (Y81S), the former gave hydroxygeranylgeranyl diphosphate as the main double-condensation product together with hydroxyfarnesyl diphosphate as a single-condensation product and a small amount of hydroxygeranylfarnesyl diphosphate as a triple-condensation product.

Pre-surgical identification of epileptogenic areas in temporal lobe epilepsy by 123I-iomazenil SPECT: a comparison with IMP SPECT and FDG PET.

Objectives: The aim of this study was to evaluate the usefulness of (123)I-iomazenil (IMZ) single photon emission computed tomography (SPECT) for the pre-surgical identification of epileptogenic areas in patients with temporal lobe epilepsy and to compare the results with those of (123)I-IMP SPECT and (18)Fluorodeoxyglucose positron emission tomography (FDG PET).

Methods: We examined seven patients with medically refractory temporal lobe epilepsy (five men and two women; mean age, 28 years) with no remarkable findings on magnetic resonance imaging. Before surgery, IMZ SPECT, IMP SPECT and FDG PET were all performed in the interictal state.

Molecular characterization of Anisakis pegreffii larvae in Pacific cod in Japan.

It is now recognized that the morphospecies Anisakis simplex is not a single species but a complex composed of three sibling species, A. simplex sensu stricto, A. pegreffii and A.