The History of the Data Systems AutoChemist® (ACH) and AutoChemist- PRISMA (PRISMA®): from 1964 to 1986.

Objectives: This paper presents the history of data system development steps (1964 - 1986) for the clinical analyzers AutoChemist®, and its successor AutoChemist PRISMA® (PRogrammable Individually Selective Modular Analyzer). The paper also partly recounts the history of development steps of the minicomputer PDP 8 from Digital Equipment. The first PDP 8 had 4 core memory boards of 1 K each and was large as a typical oven baking sheet and about 10 years later, PDP 8 was a "one chip microcomputer" with a 32 K memory chip.

Characterisation of cells in regenerating cartilage from autotransplanted perichondrium. immunohistochemical expression of smooth-muscle actin, desmin, vimentin, and Ki-67.

Autotransplanted perichondrium from rib and ear sutured to the knee joints of 26 rabbits has been examined with immunohistochemistry and shows certain structural, functional, and proliferative characteristics of regenerating cartilage. Cryostat sections have been examined for the expression of smooth-muscle actin (SMA), desmin, vimentin, and Ki-67. In this rabbit model of perichondrial grafting SMA staining showed vivid vessel regeneration, particularly in the proliferating stage about two to three weeks after grafting, and no vessels in more mature parts one month or more after transplantation.

Tendency to capsular contracture around smooth and textured gel-filled silicone mammary implants: a five-year follow-up.

The aim of this prospective, controlled clinical investigation was to compare the capsular contracture rate between silicone implants with a smooth and those with a textured surface. The implants were otherwise identical. Twenty-five women with bilateral mammary hypoplasia underwent augmentation mammoplasty.

Expression of platelet-derived growth factor beta receptor in chondrogenesis of perichondrial transplants.

An experimental study was done in two rabbits to establish whether the early stages of cartilage generation involves stimulation via the platelet-derived growth factor beta receptor (PDGFR-beta). Rib perichondrium was dissected off and transplanted to the knee joint. The perichondrial grafts were removed for microscopic investigation at day 0 and day 6 and kept frozen until analysed by immunohistochemistry and in situ hybridisation for the presence of PDGF beta receptor protein and mRNA, respectively.

Avoiding blow-out of the aortic stump by reinforcement with fibrin glue. A report of two cases.

Generally vascular surgeons agree that the most rational way to treat a patient with an infected aortic graft or aortoenteric fistula is excision of the graft, closure of the aortic stump and construction of an axillobifemoral bypass. Due to the feared complication of blow-out of the aortic stump, other solutions have been proposed, such as in-situ reconstruction with homologous saphenous veins or even with a synthetic graft, provided the perigraft fluid is non-purulent. Since this alternative is not always feasible, various methods to reinforce the closure of the aortic stump have been proposed.

A clinical comparison of the tendency to capsular contracture between smooth and textured gel-filled silicone mammary implants.

The aim of this prospective, controlled clinical investigation was to find out if there is a difference in the capsular contracture rate between silicone implants with a smooth or textured surface as the only difference. Twenty-five women with bilateral mammary hypoplasia underwent mammary augmentation. All got a textured implant on one side and a smooth implant on the other.

The effect on the preoperative symptoms and the late results of Skoog's reduction mammaplasty. A follow-up study on 149 patients.

A retrospective study has been carried out on 149 patients with hypertrophic breasts operated on during the period 1977 to 1979 using Skoog's technique of reduction mammaplasty. Both physical and psychological symptoms were investigated as well as the follow-up results of the operation as a whole, as judged by the patient herself. The observation time was 5 years and the average age of the patients was 39 years.

The effect of growth factors and synovial fluid on chondrogenesis in perichondrium.

Reconstruction of cartilage with perichondrium depends on the chondrogenic property of the perichondrial fibrocytes. The present investigation concerns the conditions for the differentiation of fibrocytes into chondrocytes both in vivo and in vitro. For the in vivo studies specimens of rib and auricular perichondrium from adult rabbits were wrapped round silicon rods which were enclosed in dialysis bags.

Formation of chondroitin sulphate proteoglycan in cartilage regenerated from free perichondrial graft.

Perichondrium from rabbit auricular or rib cartilage was used as a free autogenous graft and transplanted either to the subcutaneous tissue of the back of the rabbit or to an experimental defect in the femur condyles. Outgrowth of new tissue, morphologically indistinguishable from cartilage, was observed after six weeks. Inorganic 35SO4, administered in vivo, was incorporated into the newly formed tissue.

Closure of nasal septal perforation with a cutaneous flap and a perichondrocutaneous graft.

A cutaneous flap from the cheek and a perichondrocutaneous free graft is used for closing a perforation of the nasal septum. The cutaneous flap is rotated to set into the defect covering one side of the perforation while the other side is covered with a free perichondrocutaneous graft, nutrified by vascular proliferation from the cutaneous flap. The anterior side of the auricular concha is used as donor site for the composite perichondrocutaneous graft.

Topical analgesia for the cutting of split-skin grafts: a multicenter comparison of two doses of a lidocaine/prilocaine cream.

The topical analgesic effect of two doses of a local anesthetic cream (EMLA, Astra) in the harvesting of split-skin grafts was compared in a double-blind multicenter trial. A standardized area of 200 cm2 at the donor site of 78 patients was randomly treated with 30 or 60 gm of cream 2 to 5 hours before the operation. There was no difference in pain between the groups (p = 0.

Local anaesthetics modifying the dermal response of irradiation. An experimental study.

In a series of experiments in rabbits the dermal reaction, provoked by a single dose or intermittent doses of irradiation, was prevented or modified by topical or parenteral administration of local anaesthetics, compared to irradiated control animals. The topical application of a eutectic lidocaine/prilocaine cream, EMLA 5%, was found to be more effective than intravenously injected lidocaine (Xylocain 1%).

Experimental laryngeal reconstruction with preformed composite graft.

The use of perichondrial grafts for reconstruction of the thyroid cartilage of the larynx was studied in two series of rabbits. In the first pilot study the thyroid cartilage was replaced by a cartilage performed by the neochondrogenic effect of an auricular perichondrial graft set into the defect on a subcutaneous flap. When this transplantation technique proved successful, another series was performed where a laryngeal defect of the thyroid cartilage and the underlying mucosa were replaced by a preformed composite graft.

Perichondrial arthroplasty using fibrin glue and early mobilization. An experimental study.

An experimental study was performed in rabbits to find out whether fibrin glue, used to simplify the procedure for graft fixation in perichondrial arthroplasty, would also allow earlier mobilization of the grafted joint, thereby reducing the risk for postoperative stiffness of the joint. The results indicate the possibility of reducing the time used for postoperative fixation from three weeks originally to one week, and still achieve the same results regarding graft healing and cartilage regeneration. Immediate post-operative mobilization caused loosening of the graft and endangered the results.

Dermal effects of compositions based on the eutectic mixture of lignocaine and prilocaine (EMLA). Studies in volunteers.

The effects of the cutaneous application of EMLA cream (a eutectic mixture of lignocaine and prilocaine in their base form) were studied in volunteers. When tested by pin-prick, EMLA cream 2.5% and 5% produced analgesia of the area tested, the cream being most effective if left in contact with the skin for 60 min.

Augmentation mammaplasty: the surgical and psychological effects of the operation and prediction of the result.

The aim of this research was to evaluate the effect of augmentation mammaplasty on women and to determine if it is possible to predict which women will benefit most from the operation. Interviews were conducted with both women who were about to undergo augmentation mammaplasty and those who had undergone the surgery. These interviews were compared with interviews from a control group composed of a normally distributed population.

An anaesthetic lidocaine/prilocaine cream (EMLA) for epicutaneous application tested for cutting split skin grafts.

A 5% eutectic mixture of the two local anaesthetics lidocaine and prilocaine (EMLA, Astra Läkemedel AB) has been tested for application to the skin in the removal of split skin grafts. EMLA is an oil-in-water emulsion cream, containing 50 mg of active substances per ml (25 mg lidocaine, 25 mg prilocaine). The cream has been used on the donor sites of 146 patients and was applied a minimum of 1 h 30 min before surgery.

Augmentation mammaplasty: the surgical and psychological effects of the operation and prediction of the result.

The aim of this research was to evaluate the effect of augmentation mammaplasty on women and to determine if it is possible to predict which women will benefit most from the operation. Interviews were conducted with both women who were about to undergo augmentation mammaplasty and those who had undergone the surgery. These interviews were compared with interviews from a control group composed of a normally distributed population.

The early development of articular cartilage after perichondrial grafting.

In an experimental series of 39 rabbits the articular cartilage of the femur condyles was resected and the defect covered by a perichondrial graft from rib cartilage. The graft was fixed to the bony surface by using Tisseal (Immuno AG) and the joint was immobilized for two weeks. Beginning 24 hours postoperatively the neochondrogenesis from the perichondrial graft was analyzed histologically, sacrificing the rabbits with 48 hours interval.

Prevention of tracheal stricture in end-to-end anastomosis.

The development of stenosis at the suture line after tracheal resection and end-to-end anastomosis of the trachea is associated with failure to approximate the tracheal ends. Evidently, stenosis invariably will occur if the viable mucosal edges are not properly adapted to each other. We developed a method of anastomosis affording accurate approximation of the tracheal ends and particularly of the mucosa by doing Z-plasties in the mucosa.

Reconstructing the antihelix of protruding ears by perichondrioplasty: a modified technique.

Using the experiences achieved by experimental studies on cauliflower ear, protruding ears have been operated on with a technique involving the neochondrogenic potential of the perichondrium. The underdeveloped or absent conchoscaphal angle is restored to a normal antihelix in a posterior approach by a perichondrioplasty, gently folding the cartilage. The mobilized perichondrium is used to generate new cartilage, molding the folded cartilage in the desired position.

New anaesthetic formulation for epicutaneous application tested for cutting split skin grafts.

Ane-Pad (A 2358, Astra Lakemedel AB), a new local anesthetic formulation for application to the skin has been tested in the removal of split skin grafts. Ane-Pad is a thin cotton pad, containing a solution of 10% ketocaine base in a solvent mixture of isopropanol, glycerol and water. The pad has been used on the donor sites of 173 patients with a minimum application time of 1 h before surgery.

Reconstruction of articular cartilage with free autologous perichondrial grafts. An experimental study in rabbits.

An experimental study in adult rabbits has been performed to find out whether the cartilage forming capacity of the perichondrium could be utilized in reconstruction of articular cartilage. The normal articular cartilage of the glenoid surface of the humero-scapular joint was completely removed. Auricular perichondrium was grafted to cover the exposed bony surface with the active chondrogenic layer of the perichondrial graft facing the joint cavity.

Cartilage regeneration from perichondrium. Experimental studies and clinical applications.

Animal experiments with flaps and free grafts of perichondrium have produced new cartilage. The presence of blood seems to promote the formation of new cartilage in such instances. These perichondrial grafts have been used to produce better contours of the auricular cartilage in congenital deformities, and to reconstruct missing parts of the human ear.