Identification of Synaptic Patterns of NMDA Receptor Subtypes Upon Direction-Selective Rabbit Retinal Ganglion Cells.

Purpose: The objective of this study was to identify anisotropies that contribute to the directional preference of direction-selective retinal ganglion cells (DS RGCs) in the rabbit retina. We investigated the distributions of N-methyl-d-aspartate receptor 1 (NMDAR1), NMDAR2A and NMDAR2B receptor subunits in the dendritic arbors of rabbit DS RGCs.

Methods: The distributions of the NMDAR subunits on the DS RGCs were determined using immunocytochemistry.

Identification of synaptic pattern of kainate glutamate receptor subtypes on direction-selective retinal ganglion cells.

In this article we investigate the distributions of kainate glutamate receptor subtypes GluR5-7 and KA1, 2 on the dendritic arbors of direction-selective (DS) retinal ganglion cells (RGCs) of the rabbit retina to search for anisotropies, which might contribute to a directional preference of DS RGCs. The distribution of the kainate receptor subunits on the DS RGCs was determined using antibody immunocytochemistry. DS RGCs were injected with Lucifer yellow and the cells were identified by their characteristic morphology.

Synaptic pattern of AMPA receptor subtypes upon direction-selective retinal ganglion cells.

In the search for anisotropies that might contribute to a directional preference of direction-selective (DS) retinal ganglion cells (RGCs), we studied the distributions of AMPA receptor subtypes GluR1, GluR2/3, and GluR4 upon the dendritic arbors of DS RGCs of the rabbit with antibody immunocytochemistry. DS RGCs were injected with Lucifer yellow and the cells were identified by their characteristic morphology. The double-labeled images of dendrites and receptors were visualized by confocal microscopy and were reconstructed from high-resolution confocal images.

Alterations in the localization of calbindin D28K-, calretinin-, and parvalbumin-immunoreactive neurons of rabbit retinal ganglion cell layer from ischemia and reperfusion.

Calcium-binding proteins are thought to play important roles in calcium buffering. The present study investigated the effects of ischemia and reperfusion on calbindin D28K, calretinin, and parvalbumin immunoreactivity in the ganglion cell layer of the rabbit. Rabbits were administered ischemic damage by increasing the intraocular pressure.

Morphology of calretinin-immunoreactive neurons in the superficial layers of hamster superior colliculus after enucleation: lack of co-localization with GABA.

We recently reported on the distribution and effects of eye enucleation on the immunoreactivity of calretinin in the superficial layers of the hamster superior colliculus (SC). In the present study, we describe the types of labeled cells and compare this labeling to that of GABA, the major inhibitory neurotransmitter in the central nervous system. An almost complete depletion of calretinin-immunoreactive (IR) fibers in the superficial layers of the contralateral SC was found following unilateral enucleation.