[Antibodies against Trypanosoma cruzi in intact mice and their oncoprotective effect].

Trypanosoma cruzi antibodies that are important in the anticancer effect of this species of Trypanosoma were first detected in 14% of the 374 examined intact mice. These background antibodies were polyclonal, i.e.

[Immunization against Trypanosoma cruzi and tumor growth in mice].

The evidence has been produced that immunological mechanisms are involved in the known anticancer phenomenon of T. cruzi. Non-inbred albino mice were immunized with avirulent cultures of three strains and seven clones and then transplanted a tumor--sarcoma-180 or Ehrlich's adenocarcinoma.

[Anticancer properties of flagellate protozoan Trypanosoma cruzi Chagas, 1909].

The anticancer activity of Trypanosoma cruzi has been confirmed by the example of seven strains. Five virulent strains induced the infection, which inhibited sarcoma-180 growth 1.5-22.

[The effect of carnosine on the intracellular pH in cultured human embryonic lung fibroblasts].

Dipeptide carnosine effect on fibroblast proliferation was assessed by changes in intracellular pH (pHi) in human cultivated lung embryonal fibroblasts. pHi was defined by means of fluorescein diacetate (FDA) during 1-7 days of the culture growth. The investigation showed changes in pHi depending on dipeptide concentration and the presence of additives in it.

[Intravital determination of pH gradient between the cytoplasm and lysosomes in human fibroblasts in the normal state and in hereditary storage diseases].

Presented are the results of measurements of pH in cytoplasm and lysosomes of skin fibroblasts of healthy donors and patients with lysosomal storage diseases, mannosidosis, Fabry, Krabbe disease. The pH value was estimated in the stationary phase of growth using neutral red (lysosomes) and fluorescein diacetate (cytoplasm). It was shown that the cytoplasmic pH value in pathological cells didn't virtually differ from the control values.

Estimation and comparison of lysosomal and cytoplasmic pH of human fibroblasts from healthy donors and patients with lysosomal storage diseases.

Measurements were made of the pH of cytoplasm and lysosomes of cultured skin fibroblasts from healthy donors and from patients with lysosomal storage diseases (mannosidosis, Fabry's disease, and Krabbe's disease), and the effects of sucrose loading on normal fibroblasts were studied. The cytoplasmic pH of the pathological cells did not differ from control values, but the intralysosomal pH was significantly higher in sucrose-loaded normal fibroblasts and in cells from a patient with mannosidosis and from another with Fabry's disease. The change in pH observed accorded with an increase in size of the organelles, owing to accumulation of nonhydrolyzable compounds.

[Seasonal changes in lysosomal pH of peripheral blood cells of healthy subjects].

We studied pH values in lysosomes of peripheral blood white cells of 49 blood donors using the two-wavelengths (470 and 450 nm) microspectrophotometry of neutral red after supravital in vitro staining. In polymorphonuclear leucocytes lysosomal pH reaches the highest values in August-September (6.98 +/- 0.

[Role of intracellular pH in the development of resistance of leukemic cells to antineoplastic drugs].

Intracellular pH (pHi) of mouse lymphoid leukemia P388 cells was measured at the wavelength of 518 and 570 nm of fluorescein: pHi of rubomycin-sensitive P388 cells was higher than pHi of the cells resistant to this antitumour antibiotic (6.98 +/- 0.04 and 6.

[A pH increase in human fibroblast lysosomes during accumulation of non-hydrolysable compounds].

The changes in intralysosomal pH were measured in the stationary phase of normal human embryonic fibroblast growth under sucrose loading over a period of 6 to 120 hours and in cells with a typical lysosomal storage pathology, Fabry's disease, using a vital indicator dye, neutral red. It was shown that long-term hypertrophy of the lysosomal compartment during intracellular accumulation of non-hydrolysable compounds is concomitant with a pH increase, on the average, by 0.4 units.

[Spatial and temporal heterogeneity of pH values in lysosomes from different types of cells cultured in situ].

Distinct variety of the pH value, at the ranges of pH 4.5-7.0, was found in lysosomes.

[pH dynamics in the lysosomes and cytoplasm of tissue culture cells measured intravitally using the indicator dye neutral red].

A method of pH determination in the cytoplasm and individual lysosomes of intact living cultured cells is proposed using an indicator dye--neutral red (NR). The pH value of an investigated structure stained with NR is from the ratio between its optical densities at two wave lengths, the one corresponding to the isobestic point (lambda = 470 nm), and the other--to the absorption maximum of the neutral form of the dye (lambda = 450 nm). This method enabled us to make pH determination in the range from 4.