Complexes of angiotensin IV with functionally different proteins in the regulation of drinking behavior and hemodynamics in rats.

We compared physiological activity of synthetic complexes from angiotensin IV and functionally different proteins (transport protein, bovine serum albumin; and neurospecific Ca(2+)-binding protein, S100b) as model analogues of endogenous protein-peptide complexes. Physiological activity of angiotensin IV was specifically modified by these proteins. Our results suggest that complexes of angiotensin IV with bovine serum albumin and S100b are strong factors for the integration of central and peripheral functions at the homeostatic and behavioral level.

Physiological effects of complexes of angiotensins with functionally different carrier proteins.

We compared activity of synthetic complexes of angiotensin II and functionally different proteins (transport protein, serum albumin and neurospecific Ca2+-binding protein S100b) as analogues of endogenous protein-peptide complexes. Physiological activity of angiotensin II was specifically modified by these proteins. It was hypothesized that the complex of angiotensin II and S100b is primarily involved in the regulation of hemodynamics, whereas the complex of angiotensin II and bovine serum albumin plays a role in the formation and realization of drinking behavior.

Long-lasting changes of albino rats behavior and brain bioamines content after immunization against cholecystokinin-3 and -4.

Active immunization against cholecystokinin fragments 31-33 (CCK-3) and 30-33 (CCK-4) results in long-lasting changes of albino rats' behavior. CCK-3 and CCK-4 covalently linked to antigen-carrier evokes the suppression of the anxiety, decreases some signs of depression-like behavior and changes the level of bioamines and their catabolites in striatum at least for two months after immunization. These data can provide a perspective approach to the problem of long term correction of behavior.

An immunochemical approach to model and long-term suppression of depressive and anxiety behavior.

The inhibitor of monoaminooxydase isatin and the ligand of B-receptors cholecystokinin-4 play a significant role in the suppression and induction of depressive and anxiety states. We induced the formation of auto-antibodies to these compounds against their conjugates with antigen-carrier by immunization of white rats. The result was long-term (more than 2 months) stimulation of depressive and anxiety behavior after immunization to isatin and, in contrast, the suppression of such behavior after immunization to cholecystokinin.

[Regulatory peptides in system mechanisms of goal-directed behavior: experience of physiological activity study].

The paper presents some new data and original approaches to study of the role of opioid and vasoactive peptides in the integrative functioning of the brain. The authors performed a comparative analysis of the physiological activity of native and complex (combined with protein) forms of these biologically active substances, and discuss a possible role of autoimmune processes in peptide self-regulation of goal-directed behavior.

Protein-peptide complexes of angiotensins in the mechanisms of thirst motivation.

A comparative analysis of the physiological actions of native angiotensin I and angiotensin II and protein-peptide complexes of angiotensin I and angiotensin II on drinking behavior in rats was performed. The protein-peptide complexes of angiotensin I and angiotensin II had wider spectra of physiological activity than the native peptides. Protein-conjugated angiotensin I, unlike the motivationally neutral native angiotensin I, produced marked activation of innate drinking behavior in mice.

[Administration of the omega-3 polyunsaturated fatty acid drug eiferol decreases alcohol motivation in albino rats by elevating the level of antibodies to alcohol dehydrogenase].

The study experimentally assessed the approach proposed by the authors to lower alcohol motivation, which involves enhancement of a specific immunity at the stage of alcoholization when acetaldehydemodified ethanol exchange enzymes [alcohol dehydrogenase (ADH)] and acetaldehyde dehydrogenase may be expected to occur. Omega-3 polyunsaturated fatty acid (PUFA) drugs enhance the formation of autoantibodies to modified ADH and decrease the activity of ADH in the stomach and liver. At the same time, PUFA drugs can, under certain conditions, produce an anti-alcoholic activity and a positive effect on the psychoemotional status of animals after the ethanol deprivation period.

[Peptide-protein complexes of angiotensins in the mechanisms of thirst].

The comparative analysis of learned and natural drinking behavior under native angiotensin-I (A-I), angiotensin-II (A-II) and its protein-peptides complexes (PPC) administration in rats, was performed. Various effects of these substances on drinking behavior were observed. PPC of A-I became a dipsogenic agent as compared with the native one.

The effects of immunization against cholecystokinin fragment 30-33 in the behavior of white rats.

Active immunization of white rats with cholecystokinin-4 covalently linked to the antigen carrier BSA evoked long-lasting changes in the rats' behavior, which were in the opposite direction to the anxiogenic effects of cholecystokinin-4 itself, showing that immunization had anxiolytic effects. Immunoenzyme analysis demonstrated the presence of antibodies to cholecystokinin-4 in the serum of immunized rats. These data are interesting from the point of view of correcting pathological anxiety and fear states by inverse immunoregulation.

[Mechanisms of suppression of alcohol motivation after immunization of albino rats against alcohol dehydrogenase I: The role of ADH epitopes and ADH activity in the adrenal glands].

Experimental results have demonstrated a significant decrease in the level of alcohol consumption by albino rats immunized with heterologous horse alcohol dehydrogenase. The role of ADH epitopes 9-14, 93-115, and 265-276 in this phenomenon was examined, and it was established that the latter sequence (265-276) plays the biggest role. The inhibition of ADH activity in the adrenals of immunized rats was much higher compared to the liver.

[Protein-peptide complexes in the mechanisms of inborn and learned behavior patterns].

Protein-peptide complexes involved in learned and natural drinking behavioral patterns were comparatively analyzed in rats. Active immunization with protein-conjugated angiotensin II and beta-endorphin produces some behavioral responses. It is suggested that protein complexes of these peptides play a specific informational role in the systemic organization of learned behavior.

Main ethanol metabolizing alcohol dehydrogenases (ADH I and ADH IV): biochemical functions and the physiological manifestation.

The range of the biochemical reactions which can be catalyzed by ADH I and ADH IV is extremely wide. The most characterized functions of these enzymes are protection against excess endogenous acetaldehyde, products of lipid peroxidation, exogenous alcohols and some xenobiotics. It was found also that ADH I and ADH IV are important members of the enzyme system synthesizing retinoic acid (especially during embryogenesis).

[Effect of immunization to cholecystokinin fragment (30-33) on the behavior of albino rats].

Active immunisation of albino rats by the BSA-conjugated CCK-4 induced formation of antibodies to the CCK-4 and some long-term changes of the rat behaviour. These changes were contrary to anxiogenic effect of the CCK-4 and demonstrated an anxiolytic effect of the immunisation. The data obtained suggest a possibility of an immunocorrection of pathological anxiety and fear by an inverse immunoregulation.

Pargyline conjugate-induced long-term activation of monoamine oxidase as an immunological model for depression.

We describe an animal model of long-term depression based on active immunization of albino rats against BSA-conjugated inhibitor of monoamine oxidase, antidepressant, pargyline. Immunization resulted in anti-pargyline antibody formation and significant activation of monoamine oxidase A in brain. Immunized rats demonstrated potent decrease of motor, orientation and exploratory activity, increase of the immobility time ("despair") in Porsolt test, as well as notable signs of fear and anxiety in elevated plus maze.

Immunization against monoamine oxidase slows extinction of a conditioned active escape reflex in rats.

Immunization of white rats against bovine plasma monoamine oxidase increased the level of retention of a developed active escape reflex. This phenomenon can serve as a basis for determining the physiological role of bovine plasma monoamine oxidase.

[Induction of long-term depression (with anxiety and fear components) by immunization of rats against pargyline].

The active immunization of albino rats against pargyline (a MAO B inhibitor) induced the formation of antibody to pargyline and results in deep depressive changes and fear. These changes were observed within 6 weeks after the first immunization. Therefore, it opens the possibility to model depression long by exerting the minimum influences.

[Immunization to monoamine oxidase slows the extinction of the active avoidance conditioned reflex in rats].

Immunisation of albino rats against heterologous serum MAO from bovine plasma suppressed extinction of active avoidance response. This phenomenon can be used for elucidation of physiological role of the serum monoaminoxydase.

Formation of taste aversion and preference in protein synthesis inhibition in rats.

The investigation was devoted to the role of the synthesis of protein and peptide factors during the formation of chemosensory memory in rats. Two models of gustatory memorization were used: conditioned taste aversion (CTA), induced by the association of the taste of saccharine with a toxic injection of lithium chloride, and enhanced taste preference (ETP), induced by the influence of preliminary drinking of a saccharine solution on its repeat consumption. It was found that, under conditions of the inhibition of protein synthesis in the brain of 43% by cycloheximide and of 59% by 8-azaguanine, CTA does not form.

[The formation of taste aversion and preference under conditions of protein synthesis inhibition in rats].

The study is devoted to the role of protein synthesis in formation of chemosensory memory in rats. Two experimental models of taste memorizing were used, i.e.

[Current state of the hypothesis on functional continuum of regulatory peptides].

Further development of the hypothesis of the functional continuum of regulatory peptides is given on the basis of new experimental evidence. Some basic schemes of the regulatory chains and cascades are considered, which explain some long-term involved physiological effects of short-living regulatory peptides.

The role of dermorphin in the regulation of the processes of hibernation in mammals.

Data are presented in this paper regarding the influence of the opioid peptide, dermorphin, and its conjugate on the conditioned reflex activity of sousliks. The administration of dermorphin (0.1 mu/g, subcutaneously) is accompanied by a fall in body temperature, slowing of the baseline vegetative indices, and the sinking of the animal into a sleep-like state.