Active phytoextraction of toluene shifts the microbiome and enhances degradation capacity in hybrid poplar.

Hybrid poplars are widely recognized for their effectiveness in remediating subsurface aromatic hydrocarbon contaminants, including benzene, toluene, ethylbenzene, and xylene isomers (BTEX). While BTEX compounds are frequently found in the transpiration streams of poplars at contaminated sites, the microbial dynamics within these trees, particularly in response to hydrocarbon exposure, remain underexplored. This study utilized high-throughput amplicon sequencing to investigate the trunk microbiome in hybrid poplars at a field-scale toluene phytoremediation site.

Language Justice as Health Equity in Palliative Care: A Scoping Review.

Context: Communication is the foundation of optimal healthcare provision. Linguistic diversity is a reality in palliative care settings.

Objectives: To identify the state of the literature on language interpreting in palliative care and to examine inclusion of stakeholders in dissemination products.

Differential impact of infection on the microbiota of and .

The Western honey bee () is a vital agricultural pollinator whose populations are threatened by the parasitic mite destructor and associated pathogens. While the impact of species on honey bees, particularly larvae causing American foulbrood, is documented, their effect on the microbiota of mites remains unclear. This study aimed to investigate the influence of sp.

Fecal microbiota of diarrheic calves: Before, during, and after recovering from disease.

Background: It is unknown if gastrointestinal dysbiosis in diarrheic calves causes disease or is a consequence of the disease.

Objectives: Describe the fecal microbiota of calves before, during, and after recovering from diarrhea.

Animals: Fifteen female Holstein calves of 0 to 21 days old from a single farm.

Differential interactions of Rickettsia species with tick microbiota in Rh. sanguineus and Rh. turanicus.

Tick-borne rickettsioses, caused by Gram-negative bacteria of the Rickettsia genus, pose a growing global threat, with various arthropod vectors contributing to their transmission. Understanding the complex interactions within tick microbiota, including the role of Rickettsia species, is crucial for elucidating the dynamics of rickettsial diseases. Here, we investigate the taxonomic profiles and co-occurrence networks of Rickettsia in Rh.

Functional redundancy and niche specialization in honeybee and Varroa microbiomes.

The honeybee (Apis mellifera) is a key pollinator critical to global agriculture, facing threats from various stressors, including the ectoparasitic Varroa mite (Varroa destructor). Previous studies have identified shared bacteria between Varroa mites and honeybees, yet it remains unclear if these bacteria assemble similarly in both species. This study builds on existing knowledge by investigating co-occurrence patterns in the microbiomes of both Varroa mites and honeybees, shedding light on potential interactions.

Microbiota-driven vaccination in soft ticks: Implications for survival, fitness and reproductive capabilities in Ornithodoros moubata.

The Ornithodoros moubata (Om) soft tick, a vector for diseases like tick-borne human relapsing fever and African swine fever, poses challenges to conventional control methods. With diminishing insecticide efficacy, harnessing the tick's microbiota through innovative approaches like microbiota-driven vaccination emerges as a promising strategy for sustainable and targeted disease control. This study investigated the intricate relationship between Pseudomonas, a keystone taxon in the Om microbiome, and its impact on tick fitness, microbiome structure and network dynamics.

Differential nested patterns of Anaplasma marginale and Coxiella-like endosymbiont across Rhipicephalus microplus ontogeny.

Understanding the intricate ecological interactions within the microbiome of arthropod vectors is crucial for elucidating disease transmission dynamics and developing effective control strategies. In this study, we investigated the ecological roles of Coxiella-like endosymbiont (CLE) and Anaplasma marginale across larval, nymphal, and adult stages of Rhipicephalus microplus. We hypothesized that CLE would show a stable, nested pattern reflecting co-evolution with the tick host, while A.

Exploring the impact of on colonization resistance of microbiota using network node manipulation.

Upon ingestion from an infected host, tick-borne pathogens (TBPs) have to overcome colonization resistance, a defense mechanism by which tick microbiota prevent microbial invasions. Previous studies have shown that the pathogen alters the microbiota composition of the nymphs of , but its impact on tick colonization resistance remains unclear. We analyzed tick microbiome genetic data using published Illumina 16S rRNA sequences, assessing microbial diversity within ticks (alpha diversity) through species richness, evenness, and phylogenetic diversity.

Dynamic nesting of in the microbial communities of .

Interactions within the tick microbiome involving symbionts, commensals, and tick-borne pathogens (TBPs) play a pivotal role in disease ecology. This study explored temporal changes in the microbiome of , an important cattle tick vector, focusing on its interaction with . To overcome limitations inherent in sampling methods relying on questing ticks, which may not consistently reflect pathogen presence due to variations in exposure to infected hosts in nature, our study focused on ticks fed on chronically infected cattle.

Route of exposure to veterinary products in bees: Unraveling pasture's impact on avermectin exposure and tolerance in stingless bees.

Deforestation rapidly increases in tropical regions, primarily driven by converting natural habitats into pastures for extensive cattle ranching. This landscape transformation, coupled with pesticide use, are key drivers of bee population decline. Here, we investigate the impact of pasture-dominated landscapes on colony performance, pesticide exposure, and insecticide sensitivity of the stingless bee .

Tick-borne viruses and their risk to public health in the Caribbean: Spotlight on bats as reservoirs in Cuba.

In recent decades, tick-borne diseases (TBDs) have surged and expanded globally due to factors like changes in human activities, land use patterns, and climate change, and it have been associated with the emergence of zoonotic diseases. Cuba faces the impact of ticks on human health and the economy. Although Cuba has studied TBDs extensively for the past 50 years, focus on tick-borne viral pathogens affecting humans remains scant.

Real-world evidence of rabies post-exposure prophylaxis in Serbia: Nation-wide observational study (2017-2019).

Background: Rabies remains a deadly zoonotic disease, primarily prevalent in Eastern European countries, with a significant global burden in Asia and Africa. Post-exposure prophylaxis (PEP) is critical to prevent clinical rabies. Serbia, a country with a relatively low animal rabies incidence, has been implementing a 4-dose Essen PEP regimen for 13 years.

Impact of Infection on the Colonization Resistance of Bird Gut Microbiota: A Preliminary Study.

Avian malaria infection has been known to affect host microbiota, but the impact of infection on the colonization resistance in bird gut microbiota remains unexplored. This study investigated the dynamics of infection in canaries, aiming to explore the hypothesis that microbiota modulation by would reduce colonization resistance. Canaries were infected with , while a control group was maintained.

Microfluidic PCR and network analysis reveals complex tick-borne pathogen interactions in the tropics.

Background: Ixodid ticks, particularly Rhipicephalus sanguineus s.l., are important vectors of various disease-causing agents in dogs and humans in Cuba.

Dissecting the impact of Anaplasma phagocytophilum infection on functional networks and community stability of the tick microbiome.

Context: Pathogens can manipulate microbial interactions to ensure survival, potentially altering the functional patterns and microbiome assembly. The present study investigates how Anaplasma phagocytophilum infection affects the functional diversity, composition, and assembly of the Ixodes scapularis microbiome, with a focus on high central pathways-those characterized by elevated values in centrality metrics such as eigenvector, betweenness, and degree measures, in the microbial community.

Methods: Using previously published data from nymphs' gut V4 region's amplicons of bacterial 16S rRNA, we predicted the functional diversity and composition in control and A.

Hierarchical shift of the Aedes albopictus microbiota caused by antimicrobiota vaccine increases fecundity and egg-hatching rate in female mosquitoes.

Recent studies show that mosquito-microbiota interactions affects vector competence and fitness. We investigated if host antibodies modifying microbiota impact mosquito physiology. We focused on three prevalent bacteria (Acinetobacter, Pantoea, and Chryseobacterium), originally isolated from the Asian tiger mosquito Aedes albopictus.

Conserved core microbiota in managed and free-ranging elephants.

The gut microbiota plays a crucial role in animal health and homeostasis, particularly in endangered species conservation. This study investigated the fecal microbiota composition of European captive-bred African savanna elephants () housed in French zoos, and compared it with wild African savanna elephants. Fecal samples were collected and processed for DNA extraction and amplicon sequencing of the 16S rRNA gene.

Transovarial Transmission of in () Ticks Results in a Bottleneck for Strain Diversity.

is an obligate intraerythrocytic bacterium of bovines, responsible for large economic losses worldwide. It is mainly transmitted by ticks and, despite mounting evidence suggesting transovarial transmission, the occurrence of this phenomenon remains controversial. We evaluated the vector competence of larvae vertically infected with to transmit the bacterium to a naïve bovine.

Microbiota perturbation by anti-microbiota vaccine reduces the colonization of Borrelia afzelii in Ixodes ricinus.

Background: Ticks can transmit a broad variety of pathogens of medical importance, including Borrelia afzelii, the causative agent of Lyme borreliosis in Europe. Tick microbiota is an important factor modulating, not only vector physiology, but also the vector competence. Anti-microbiota vaccines targeting keystone taxa of tick microbiota can alter tick feeding and modulate the taxonomic and functional profiles of bacterial communities in the vector.