Impact of human papillomavirus infection on semen parameters and reproductive outcomes.

Background: Human papillomavirus (HPV) has been shown to adversely affect human reproduction. We aimed to evaluate the prevalence of human papillomavirus (HPV) infection in men and its correlation with semen parameters and reproductive outcomes.

Methods: Semen samples and penile swabs were collected from potential sperm donors (SD, n = 97) and male partners of infertile couples (IM, n = 328).

Prevalence and genotype distribution of human papillomavirus in Czech non-vaccinated heterosexual couples.

Background: Data about the genotype-specific human papillomavirus (HPV) prevalence in the Czech Republic is limited. We aimed to evaluate the prevalence and concordance of genotype-specific HPV infection detected in semen samples, penile swabs and cervical swabs from non-vaccinated heterosexual couples without HPV-associated disease.

Methods: Semen samples and penile swabs were collected from male partners and cervical swabs were collected from female partners of heterosexual couples treated for infertility (n = 195).

A Global Survey of Reproductive Specialists to Determine the Clinical Utility of Oxidative Stress Testing and Antioxidant Use in Male Infertility.

Purpose: The use of antioxidants is common practice in the management of infertile patients. However, there are no established guidelines by professional societies on antioxidant use for male infertility.

Materials And Methods: Using an online survey, this study aimed to evaluate the practice pattern of reproductive specialists to determine the clinical utility of oxidative stress (OS) testing and antioxidant prescriptions to treat male infertility.

Prevalence of human papillomavirus infection in oocyte donors and women treated for infertility: An observational laboratory-based study.

Objective: The aims of this study were to determine the prevalence of human papillomavirus (HPV) infection in women treated for infertility and oocyte donors, and to investigate the possible influence of HPV infection on reproductive outcomes.

Study Design: In this observational laboratory-based study, cervical swabs were collected from oocyte donors (n = 207), and women treated for infertility (n = 945) and analysed for the presence of high-risk HPV (hrHPV) genotypes using the cobas 4800 HPV Test and PapilloCheck HPV-Screening. Associations between hrHPV positive status and fertility outcome or socio-behavioral and health characteristics were evaluated using R statistical software.

Does EmbryoGlue transfer medium affect embryo transfer success rate?

Objective: Verification of the effect of EmbryoGlue (EG) transfer medium enriched with hyaluronan on the embryo transfer success rate.

Design: A prospective study.

Setting: Fertimed, Fertility Treatment Centre, Olomouc.

A Head-to-Head Analytical Comparison of Cobas 4800 HPV, PapilloCheck HPV Screening, and LMNX Genotyping Kit HPV GP for Detection of Human Papillomavirus DNA in Cervical and Cervicovaginal Swabs.

High-risk human papillomavirus (hrHPV) infection is a cause of cervical cancer development. The addition of hrHPV testing to cervical cancer screening and monitoring of cervical intraepithelial neoplasia treatment improves the efficacy of screening and treatment, respectively. Self-sampling for hrHPV testing seems a promising tool for increasing patient participation in cervical cancer screening.

HCG level after embryo transfer as a prognostic indicator of pregnancy finished with delivery.

Objective: To examine patients after embryo transfer for predictive influence of the human chorionic gonadotropin (hCG) level on the probability of finishing pregnancy with delivery.

Design: Retrospective study.

Setting: Fertimed, Fertility Treatment Centre, Olomouc.

The attitudes of IVF patients treated in the Czech Republic towards informing children born after gamete donation.

Background: In recent decades gamete donation has received growing attention. Data from the Czech National Registry of Assisted Reproduction show that the number of cycles using donated oocytes has been increasing every year. According to Czech law, gamete donation is anonymous.

[How is the Czech oocyte donor? The analysis of their profile].

Objective: The aim of our study was to investigate (a) the basic socio-demographic characteristics of Czech oocytes donors, (b) their attitudes towards anonymous or nonanonymous donation and (c) their motivations to donate oocytes.

Design: Original article.

Setting: Clinic of Reproductive Medicine and Gynecology Zlin.

[Current trends in gamete donation - psychosocial and ethical issues].

Objective: To overview contemporary knowledge of legal and psychosocial rules in gamete donation. Previously, anonymous donation was preferred and recommended by experts but currently, with respect to the right to know the genetic origin of individuals, the relation to donor anonymity was reconsidered in many countries. There is a growing tendency to introduce the open identity system in gamete donation.

[Influence of hCG glycosylation on its functions in female reproduction].

Objective: To review contemporary knowledge of the hCG molecule, its isoforms and the importance of glycosylation. Biologic variants and glycoforms of hCG have different biological activities and functions related to the control of menstrual cycle, conception, gestation as well as gynaecologic and non-gynaecologic malignancies.

Design: A review.

Effects of 2,3-Dehydrosilybin and Its Galloyl Ester and Methyl Ether Derivatives on Human Umbilical Vein Endothelial Cells.

The effects in vitro of 2,3-dehydrosilybin and several galloyl esters and methyl ethers on the viability, proliferation, and migration of human umbilical vein endothelial cells (HUVECs) were evaluated. The monogalloyl esters were synthesized by a chemoselective esterification method or by Steglich esterification of suitably protected 2,3-dehydrosilybin (1) with protected gallic acid. 2,3-Dehydrosilybin (1) displayed more potent cytotoxic, antiproliferative, and antimigratory activities (IC50 12.

Psychological distress and academic self-perception among international medical students: the role of peer social support.

Background: Psychological distress among medical students is commonly observed during medical education and is generally related to poor academic self-perception. We evaluated the role of peer social support at medical schools in the association between psychological distress and academic self-perception.

Methods: An online survey was conducted in a medical degree program for 138 international students educated in English in the Czech Republic.

Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay.

Aim: The aim of this prospective study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage estimated by Sperm Chromatin Structure Assay.

Methods: Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence.

[The influence of warmed infusion during C-section on the mother and child--a pilot randomised prospective study].

Aim Of The Study: To evaluate the influence of warmed infusions on the mothers and newborns well-being after C-section.

Design: Prospective randomized study.

Setting: Dept.

[The occurence of genetic trombophilic markers in patients evaluated for infertility].

Objective: To assess and compare the frequency of selected gene mutations of thrombophilic markers (FV Leiden, FII prothrombin G20210A and MTHFR C677T) in patients with primary and secondary infertility.

Design: Retrospective study.

Setting: Institute of normal anatomy, Faculty of Medicine and Dentistry, Palacky University Olomouc.

[Influence of the length of cultivation of no early cleavage embryos on the IVF success rate].

Objective Of The Study: To evaluate the success of treatment cycle after transfer of no early cleavage embryos in relation to the length of in vitro cultivation.

Type Of The Study: Retrospective study.

Setting: Dept.

Lycopene improves the distorted ratio between AA/DHA in the seminal plasma of infertile males and increases the likelihood of successful pregnancy.

Aims: Male infertility has been associated with imbalance in the polyunsaturated fatty acids arachidonic acid/docosahexaenoic acid (AA/DHA) ratio. This study aimed to assess whether use of the potent antioxidant, lycopene, would affect this imbalance in seminal plasma and to test whether modulation of the AA/DHA ratio correlates with male fertility.

Methods: The study included 44 males from infertile couples who were treated with lycopene for three months before scheduled IVF treatment.

Dehiscent scar in the lower uterine segment after Caesarean section and IVF infertility treatment: a case report.

Aims: Caesarean section is the most common obstetric operation associated with short and long term risks, one of which is uterine scar dehiscence. In this case report we describe four cases of in vitro fertilization and embryo transfer (IVF+ET) treatment where the embryo was transferred into the uterus with known scar dehiscence in the lower uterine segment after a previous Caesarean section (SC).

Methods: All transfers of embryos were ultrasound guided directly into the middle of uterine cavity.

Clinical anatomy aspects of functional 3D training -- case study.

Introduction: Functional 3D training originating in rehabilitation and physiotherapy routines, is modelled on the basis of a patient's functional impairment and muscle function test results. 3-D functional rehabilitation comprises two key components. The first is three-dimensional movements of the body and the second is restoration of function, The aim of 3D training is not only to improve physical fitness and muscular coordination, it is recommended for all age groups regardless of health status.

Synthesis and antiangiogenic activity of new silybin galloyl esters.

The synthesis of various silybin monogalloyl esters was developed, and their antiangiogenic activities were evaluated in a variety of in vitro tests with human umbilical vein endothelial cells (HUVECs). A structure-activity relationship (SAR) study found the regioselectivity of the silybin galloylation to be highly significant. Silybin (as an equimolar mixture of two diastereomers A and B) exhibited quite poor antiangiogenic activities, whereas its B stereoisomer is more active than silybin A.

A randomized controlled trial of lycopene treatment on soluble receptor for advanced glycation end products in seminal and blood plasma of normospermic men.

Problem: The aim of present study was to investigate the effects of antioxidant lycopene on soluble receptor for advanced glycation end products (sRAGE) levels in blood and seminal plasma in normospermic males.

Methods: Study included 15 fertile volunteers and 13 normospermic male partners from infertile relationships. The treatment was 12-week administration of 20 mg of lycopene or placebo followed by crossover and treatment for a further 12 weeks.

[Comparison of reactive oxygen species production in neat semen and washed spermatozoa].

Objective: To determine Reactive Oxygen Species (ROS) production in neat semen and spermatozoa suspension using chemiluminescence and to examine correlation between both methods.

Subject: Prospective laboratory study.

Setting: Department of Obstetric and Gynecology, University Hospital, Olomouc.

Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen.

Objective: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite.

Design: Prospective study.

Setting: University hospital.