Biosynthesis of Apigenin Glucosides in Engineered .

Glucosylation is a well-known approach to improve the solubility, pharmacological, and biological properties of flavonoids, making flavonoid glucosides a target for large-scale biosynthesis. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of enzymatic systems for large-scale. is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and bio-fuels.

Glucosylation of Isoeugenol and Monoterpenes in by YdhE from .

has been regarded as a food-grade microorganism. In recent years, the research to improve the activities of beneficial therapeutics and pharmaceutical substances has resulted in the engineering of the therapeutically favorable cell factory system of In this study, we successfully glucosylated isoeugenol and other monoterpene derivatives in using a promiscuous YdhE, which is a glycosyltransferase from . For efficient glucosylation, cultivation conditions such as the production time, substrate concentration, carbon source, and culture medium were optimized.

-Glucosylation in with YdhE from .

is traditionally known as a food-grade microorganism due to its high ability to produce amino acids and its endotoxin-free recombinant protein expression factory. In recent years, studies to improve the activities of useful therapeutics and pharmaceutical compounds have led to the engineering of the therapeutically advantageous cell factory system. One of the well-studied ways to improve the activities of useful compounds is glucosylation with glycosyltransferases.