A New Highly Sensitive Electrochemical Biosensor for Ethanol Detection Based on Gold Nanoparticles/Reduced Graphene Oxide/Polyallylamine Hydrochloride Nanocomposite.

A highly sensitive electrochemical biosensor for ethanol based on a screen-printed electrode modified with gold nanoparticles-electrochemically reduced graphene oxide-poly (allylamine hydrochloride) nanocomposite (AuNPs-ERGO-PAH) is reported in this work. Ethanol was oxidized in the presence of the oxidized form of the nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by alcohol dehydrogenase (ADH) immobilized in sol-gel. The AuNPs-ERGO-PAH nanocomposite was used as a transducer for the electrocatalytic oxidation of the reduced form the nicotinamide adenine dinucleotide (NADH) produced in the enzyme reaction.

Ultrasensitive Detection of Hydrogen Peroxide Using Methylene Blue Grafted on Molecular Wires as Nanozyme with Catalase-like Activity.

In this study, we present the development of an electrochemical sensor designed for ultrasensitive detection of endogenous HO. This sensor relies on signal amplification achieved through nanozyme activity exhibited by methylene blue (MB) grafted onto a peptide support. The sensor exhibited excellent selectivity and sensitivity, with a limit of detection of 18 nM and a linear detection range of 20-200 nM.

A Novel Amperometric Biosensor Based on Poly(allylamine hydrochloride) for Determination of Ethanol in Beverages.

Herein, we report on a new type of ethanol biosensor based on a screen-printed electrode modified with poly(allylamine hydrochloride). The alcohol dehydrogenase was immobilized on the surface of the sensor using the sol-gel matrix. Working parameters such as applied potential, pH, NAD concentration, storage conditions were optimized.