Rathayibacter tanaceti sp. nov., a Novel Actinobacterium from Tanacetum vulgare Infested by Foliar Nematode Aphelenchoides sp.

Two novel yellow-pigmented, rod-shaped and non-motile coryneform actinobacteria, strains VKM Ac-2596 and VKM Ac-2761, were isolated from a plant Tanacetum vulgare (Asteraceae) infested by foliar nematode Aphelenchoides sp. The strains exhibited the highest 16S rRNA gene sequence similarities to Rathayibacter agropyri CA4 (99.71%), Rathayibacter rathayi DSM 7485 (99.

Fungal Planet description sheets: 951-1041.

Novel species of fungi described in this study include those from various countries as follows: , from permafrost, from an unidentified marine sponge. , on humus in mixed forest. , Golovinomyces glandulariae on on leaves of on leaves of on leaves of , on soil, on rotten stalks of on leaf litter covered soil, (incl.

Draft Genome Sequences of New Isolates and the Known Species of the Family Microbacteriaceae Associated with Plants.

Draft genome sequences of 11 bacteria belonging to the family Microbacteriaceae were obtained using Illumina technology. The genomes of these strains have sizes from 3.14 to 4.

Draft Genome Sequence of sp. Strain VKM Ac-2630 Isolated from Leaf Gall Induced by the Knapweed Nematode on .

A draft genome sequence of sp. strain VKM Ac-2630 was derived using Ion Torrent sequencing technology. The genome size of this strain is 3.

Draft Genome Sequence of Methylophaga muralis Bur 1, a Haloalkaliphilic (Non-Methane-Utilizing) Methylotroph Isolated from a Soda Lake.

The draft genome sequence of Methylophaga muralis strain Bur 1 (VKM B-3046T), a non-methane-utilizing methylotroph isolated from a soda lake, is reported here. Strain Bur 1 possesses genes for methanol and methylamine (methylamine dehydrogenase and N-methylglutamate pathway) oxidation. Genes for the biosynthesis of ectoine were also found.

Draft Genome Sequence of Methyloligella halotolerans С2T, a New Halotolerant Methylotroph, Accumulating Poly-3-Hydroxybutyrate and Ectoine.

Methyloligella halotolerans С2 is a moderate halophilic obligate methylotroph, accumulating ultra-high-molecular-weight poly-3-hydroxybutyrate (up to 8 to 10 MDa) from methanol. Here we report a draft genome and annotation of Methyloligella halotolerans C2 (VKM B-2706 = CCUG 61687 = DSM 25045).

Draft Genome Sequence of "Rathayibacter tanaceti" Strain VKM Ac-2596 Isolated from Tanacetum vulgare Infested by a Foliar Nematode.

The draft genome of "Rathayibacter tanaceti" VKM Ac-2596 is 3.17 Mb in size with an average G+C content of 70.7% and comprises at least two nonidentical copies of ribosomal small subunit (SSU-rRNA) genes.

Comparative genome analysis of Pseudogymnoascus spp. reveals primarily clonal evolution with small genome fragments exchanged between lineages.

Background: Pseudogymnoascus spp. is a wide group of fungi lineages in the family Pseudorotiaceae including an aggressive pathogen of bats P. destructans.

[Fungal diversity in the Antarctic active layer].

Taxonomic diversity of fungi in the samples of the active layer of Antarctica was investigated using conventional microbiological techniques and metagenomic analysis of total DNA extracted from environmental samples. The list of Antarctic microscopic fungi was expanded, including detection of the species representing a portion of the fungal complex, which is nonculturable or sterile on conventional nutrient media.

[Comparative morphological, ecological, and molecular studies of Aspergillus versicolor (Vuill.) Tiraboschi strains isolated from different ecotopes].

Cultural, morphological, ecological, and trophic properties (growth at different temperatures and on various organic substrates), as well as molecular and genetic peculiarities of Aspergillus versicolor (Vuill) Tiraboschi strains of different origins, were determined. The strains were isolated from different ecotopes (upper horizons of modern soils of several geographic regions, ancient soils and peat, and permafrost). No essential distinctions in cultural and morphological properties were revealed between the strains.

Cellobiose dehydrogenase formation by filamentous fungus Chaetomium sp. INBI 2-26(-).

Laccase-negative filamentous fungus INBI 2-26(-) isolated from non-sporulating laccase-forming fungal association INBI 2-26 by means of protoplast technique was identified as Chaetomium sp. based on partial sequence of its rRNA genes. In the presence of natural cellulose sources, the strain secreted neutral cellobiose dehydrogenase (CDH) activity both in pure culture and in co-culture with laccase-positive filamentous fungus INBI 2-26(+) isolated from the same association.

[Expression of a cloned hemagglutinin-neuraminidase gene from Newcastle disease virus in murine myeloma cells].

Vaccination with autologous cancer cells expressing a potent foreign antigen is promising for immunotherapy of tumors. A construct was obtained to transfect cancer cells with the hemagglutinin-neuraminidase (HN) gene of the Newcastle disease virus (NDV). Specific primers were designed, and the HN cDNA was amplified from RNA isolated from the allantoid fluid of NDV-infected embryonated chicken eggs.

Organization of fusicoccin receptor in higher plant plasma membrane: relationship between affinity and molecular mass.

Higher plant plasma membranes carry receptors of different affinity for the phytotoxin fusicoccin. Reception of fusicoccin involves proteins belonging to the highly conserved 14-3-3 family, but the complete structure of the fusicoccin receptor (FCR) is unknown. Using radiation inactivation analysis, we estimated the molecular masses of low-affinity and high-affinity FCR at 63 +/- 7 and 130 +/- 15 kD, respectively.

[The interaction of monoclonal antibodies to the structural proteins of the Vnukovo-32 vaccinal virus with other viruses of the rabies group].

The interaction of 7 monoclonal antibodies (MCA) to the nucleocapsid complex and 3 MCA to protein G of the vaccine virus Vnukovo-32 with 33 and 27 members of the rabies virus group, respectively, was studied. The indirect immunofluorescence test showed 7 MCA to the nucleocapsid complex to recognize 4 antigenic determinants (AD). Two MCA recognized the AD common for all the viruses under study.

[The isolation and characteristics of hybridomas producing monoclonal antibodies to the structural proteins of the Vnukovo-32 strain of the rabies virus].

A series of hybridomas secreting monoclonal antibodies (MCA) to glycoprotein and nucleocapsid proteins of rabies virus strain Vnukovo-32 was selected as a result of fusion of splenocytes from immune BALB/c mice with cells of myeloma line Sp2/OAq14, screening and cloning by limiting dilution methods in semi-liquid agar. Four hybridomas secreted MCA to glycoprotein in high titres (5.0 x 10(5)-2.

[Expression of Staphylococcus aureus enterotoxin A gene in heterologous systems].

The genomic library of Staphylococcus aureus genes on the plasmid vector pSL5 has been constructed. The library contains a 2.5 kb HindIII DNA fragment including the gene for enterotoxin A.

[The cloning and expression of the Staphylococcus aureus enterotoxin A gene in Escherichia coli cells].

Gene ent-A has been cloned on phage vector pSL5 with the use of the gene library of S. aureus FR1722(H). It is located within DNA fragment Hind III having 2,500 nucleotide pairs.

Respecrins--a new type of compound with targeted action.

A new technique for the creation of compounds with targeted action is described. In such compounds, termed respecrins (receptor-specific, screened toxin), a physiologically active component carries an epitope-containing fragment of the target antigen and is screened or masked by antibody specific to this antigen. Interaction of the respecrin with the target cell results in dissociation of this immunocomplex, and thereby activation of the physiologically active component.

[The effect of x-rays on the level, composition and para-nitroanisole-o-demethylase activity of cytochrome P-450 in the liver microsomes of rats].

A study was made of the effect of X-radiation of different doses on the content of P-450 cytochrome in a microsomal fraction of rat liver. When the haemoprotein level markedly decreased an increase in Km and a decrease in Vmax were noted in the reaction of O-demethylation of para-nitroanisole by microsomes of the irradiated rat liver. It is suggested that one of the cause of the effect observed is the postirradiation change in the composition of cytochrome P-450 pool resulting from a selective decrease in the level of the radiosensitive forms of haemoprotein.