A model-based approach for mining membrane protein crystallization trials.

Motivation: Membrane proteins are known to play crucial roles in various cellular functions. Information about their function can be derived from their structure, but knowledge of these proteins is limited, as their structures are difficult to obtain. Crystallization has proved to be an essential step in the determination of macromolecular structure.

Parallel and distributed methods for incremental frequent itemset mining.

Traditional methods for data mining typically make the assumption that the data is centralized, memory-resident, and static. This assumption is no longer tenable. Such methods waste computational and input/output (I/O) resources when data is dynamic, and they impose excessive communication overhead when data is distributed.

Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes.

The chromosomal location of human constant region light chain immunoglobulin (Ig) genes has been determined by analyzing a group of human fibroblast/rodent somatic cell hybrids with nucleic acid probes prepared from cloned human kappa and lambda constant region genes. Human chromosomes in each cell line were identified by isoenzyme analysis. The DNA from hybrid cells was digested with restriction endonucleases, size fractionated by gel electrophoresis, transferred to nitrocellulose or DBM paper, and hybridized with (32)P-labeled nucleic acid probes.

Isolation of unique sequence human X chromosomal deoxyribonucleic acid.

Labeled unique sequence human X chromosomal DNA has been isolated by hybridization of labeled human DNA with DNA from a human-mouse hybrid cell line, A9/HRBC2-A, which contains a single human chromosome, the X. Homopolymer tails of poly(dA) were added to nick-translated unique sequence human DNA to permit separation of the labeled DNA from unlabeled driver DNA by binding to oligo(dT)-cellulose. Human DNA sequences homologous with mouse DNA were removed from this labeled poly-(dA)-tailed human probe by hybridization with excess unlabeled mouse DNA.