Canadian Association of Neurosciences Review: learning at a snail's pace.

While learning and memory are related, they are distinct processes each with different forms of expression and underlying molecular mechanisms. An invertebrate model system, Lymnaea stagnalis, is used to study memory formation of a non-declarative memory. We have done so because: (1) We have discovered the neural circuit that mediates an interesting and tractable behaviour; (2) This behaviour can be operantly conditioned and intermediate-term and long-term memory can be demonstrated; and (3) It is possible to demonstrate that a single neuron in the model system is a necessary site of memory formation.

The Take Control of Your Blood pressure (TCYB) study: study design and methodology.

Background: Among the 65 million Americans with hypertension, only approximately 31% have their blood pressure under control (<140/90 mm/Hg). Despite the damaging impact of hypertension and the availability of evidence-based target values for blood pressure, interventions to improve blood pressure control have had limited success.

Objectives: A randomized controlled health services intervention trial with a two by two design is being conducted to improve blood pressure control.

The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements.

Over the last decade, the introduction of microarray technology has had a profound impact on gene expression research. The publication of studies with dissimilar or altogether contradictory results, obtained using different microarray platforms to analyze identical RNA samples, has raised concerns about the reliability of this technology. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and data analysis issues.

Evaluation of external RNA controls for the assessment of microarray performance.

External RNA controls (ERCs), although important for microarray assay performance assessment, have yet to be fully implemented in the research community. As part of the MicroArray Quality Control (MAQC) study, two types of ERCs were implemented and evaluated; one was added to the total RNA in the samples before amplification and labeling; the other was added to the copyRNAs (cRNAs) before hybridization. ERC concentration-response curves were used across multiple commercial microarray platforms to identify problematic assays and potential sources of variation in the analytical process.

Surveillance of hazardous substances releases due to system interruptions, 2002.

The Hazardous Substances Emergency Events Surveillance (HSEES) system collected information on 9014 acute hazardous substance releases in 15 participating states in 2002. There were 3749 fixed-facility manufacturing events, of which 2100 involved "interruptions" to normal processing and 1649 "comparisons" that did not involve interruption. Equipment failure (69%) or intentional acts (20%) were the main root factor.

Modulation of aerial respiratory behaviour in a pond snail.

Aerial respiratory in Lymnaea is driven by a three-neuron CPG whose sufficiency and necessity has been directly demonstrated. While this CPG is 'hard-wired' it displays a tremendous amount of plasticity. That is, it is possible by employing specific training procedures to alter how it functions in a specific hypoxic environment.

Toxicogenomics and cross-species biomarker discovery: applications in drug discovery and safety assessment.

Toxicogenomics has evolved into a useful technique for providing greater mechanistic insights into adverse effects that will spur the development of novel approaches for identifying and understanding toxicity issues. The ability to capture a snapshot of the transcriptome at any given time during the development of an adverse phenotype allows unprecedented molecular views into the dynamic physiological changes that are occurring on either time or dose continuum for a toxicology study of interest advancing our basic knowledge of adverse events, and providing the necessary scientific framework for developing new strategies and tools for safety assessment programs. The development of an effective subset of cost effective devices for identifying toxicity earlier in the drug development process will help identify the most promising candidate compounds to move forward leading to a reduction in compound attrition due to toxicity.

Ultrasound-accelerated tissue fixation/processing achieves superior morphology and macromolecule integrity with storage stability.

We demonstrate that high-frequency and high-intensity ultrasound (US) can be applied to both tissue fixation and tissue processing to complete the conventional overnight formalin-fixation and paraffin-embedding (FFPE) procedures within 1 hr. US-facilitated FFPE retains superior tissue morphology and long-term room temperature storage stability than conventional FFPE. There is less alteration of protein antigenicity after US-FFPE preservation so that rapid immunohistochemical reactions occur with higher sensitivity and intensity, reducing the need for antigen retrieval pretreatment.

The peripherally inserted central catheter: what are the current indications for its use?

The use of peripherally inserted central catheters (PICCs) has dramatically increased since first being used for IV therapy in the 1980s. Currently, sales of PICCs are second only to acute care central venous catheters and exceed all other types of long-term venous access devices. The use of PICCs has also increased dramatically in the home care setting.

Inhibition of MHC class I is a virulence factor in herpes simplex virus infection of mice.

Herpes simplex virus (HSV) has a number of genes devoted to immune evasion. One such gene, ICP47, binds to the transporter associated with antigen presentation (TAP) 1/2 thereby preventing transport of viral peptides into the endoplasmic reticulum, loading of peptides onto nascent major histocompatibility complex (MHC) class I molecules, and presentation of peptides to CD8 T cells. However, ICP47 binds poorly to murine TAP1/2 and so inhibits antigen presentation by MHC class I in mice much less efficiently than in humans, limiting the utility of murine models to address the importance of MHC class I inhibition in HSV immunopathogenesis.

Higher order statistical analysis of /x/ in male speech.

This paper presents a study of kurtosis analysis for the sound /x/ in male speech, /x/ is the sound of the 'o' at the end of words such as 'ago'. The sound analysed for this paper came from the Australian National Database of Spoken Language, more specifically the male speaker 17. The /x/ was isolated and extracted from the database by the author in a quiet booth using standard multimedia software.

Surveillance of hazardous substance emergency events: identifying areas for public health prevention.

The Hazardous Substances Emergency Events Surveillance (HSEES) system is a comprehensive, state-based surveillance system of hazardous substance releases and public health consequences. Maintained by the Agency for Toxic Substances and Disease Registry (ATSDR) since 1990, the system captures information on acute releases of hazardous substances that need to be cleaned up or neutralized according to federal, state, or local law. Information about threatened releases that result in public health action such as evacuation is also included.

The veterans' study to improve the control of hypertension (V-STITCH): design and methodology.

Background: Among the 60 million Americans with hypertension, only approximately 31% have their blood pressure (BP) under control (<140/90 mm Hg). Despite the damaging impact of hypertension and the availability of evidence-based target values for BP, interventions to improve BP control have had limited success.

Objectives: A randomized controlled health services intervention trial with a split-plot design is being conducted to improve BP control.

Nurse administered telephone intervention for blood pressure control: a patient-tailored multifactorial intervention.

Objectives: A randomized controlled trial involving a nurse administered patient-tailored intervention is being conducted to improve blood pressure (BP) control.

Methods: Veterans with hypertension from an outpatient primary care clinic completed a baseline assessment and were randomly allocated to either a nurse administered intervention or to usual care. In this ongoing study, intervention patients receive the tailored intervention bi-monthly for 2 years via telephone; the goal of the intervention is to promote adherence with medication and improve health behaviors.

Oxidant-induced apoptosis in human retinal pigment epithelial cells: dependence on extracellular redox state.

Purpose: To test whether variation in extracellular cysteine (Cys) redox potential (E(h)) over the physiologic range occurring in human plasma affects oxidant-induced apoptosis in cultured human retinal pigment epithelial (hRPE) cells.

Methods: The hRPE cells were incubated in culture medium with E(h) established over the range of -16 mV (most oxidized) to -158 mV (most reduced) by adding different concentrations of Cys and cystine (CySS) with constant total Cys equivalents. Apoptosis was induced with tert-butylhydroperoxide (tBH).

Hazardous-chemical releases in the home.

Data from the Hazardous Substances Emergency Events Surveillance (HSEES) system were analyzed for the period 1996-2001 to describe the chemicals, causal factors, temporal patterns, types of adverse effects, and public health actions (e.g., an evacuation) associated with releases of hazardous chemicals in the home.

Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-beta gene promoter.

The interferon-beta promoter has been studied extensively as a model system for combinatorial transcriptional regulation. In virus-infected cells the transcription factors ATF-2, c-Jun, interferon regulatory factor (IRF)-3, IRF-7 and NF-kappaB, and the coactivators p300/CBP play critical roles in the activation of this and other promoters. It remains unclear, however, why most other combinations of AP-1, IRF and Rel proteins fail to activate the interferon-beta gene.

Bifrontal electroconvulsive therapy in the elderly: a 2-year retrospective.

Objectives: We sought to examine the clinical effect of bifrontal (BF) electroconvulsive therapy (ECT) in depressed patients aged 65 years and older.

Methods: A retrospective chart review of all patients who received BF ECT for a depressive disorder between January 2000 and December 2002 was made.

Results: Fourteen patients, with a mean age of 73.

Enrollment in clinical trials according to patients race: experience from the VA Cooperative Studies Program (1975-2000).

Background: Racial distribution of clinical trial participants is important because results from these studies serve to define evidence-based practice. This report summarizes the experience of the VA Cooperative Studies Program (CSP) in enrolling white, black and Hispanic patients.

Methods: An analysis of enrollment in randomized controlled trials conducted by VA CSP between 1975 and 2000.

Vaccination levels associated with lack of measles transmission among preschool-aged populations in the United States, 1989-1991.

Knowledge of the minimum level of vaccination capable of preventing measles transmission in an age group is helpful for establishing program targets for measles elimination. In 1990, during the measles resurgence in the United States, one-half of cases occurred in children aged <5 years. Although estimated population immunity among persons >or=6 years of age was 93%, immunity was lower and varied widely among preschool-aged children.

The impact of nursing practice on the history and effectiveness of total parenteral nutrition.

Nutrition support nursing grew out of the development of total parenteral nutrition in the late 1960s. Nurses contributed to the development of safe and effective delivery of parenteral nutrition and continue to serve on nutrition and metabolic teams in a variety of ways. Research demonstrating the positive effect of the nurse and the team are presented here, along with the history, evolution, and current state of nutrition support nursing.

Congestive heart failure patients' perceptions of quality of life: the integration of physical and psychosocial factors.

Congestive heart failure (CHF) lowers survival and worsens the quality of life (QOL) of over four million older Americans. Both clinicians and standardized instruments used to assess the QOL of patients with CHF focus primarily on physical symptoms rather than capturing the full range of psychosocial concerns. The purpose of this study was to gather descriptions of the components of QOL as understood by patients living with CHF.

Right unilateral electroconvulsive therapy at six times seizure threshold.

Objective: To examine the clinical practice of right unilateral electroconvulsive therapy (ECT) administered at six times seizure threshold (6 x RUL ECT).

Method: A retrospective review of all patients who received 6 x RUL ECT between July 2000 and June 2002.

Results: Twenty-one patients across a range of ages and diagnostic groups received D'Elia unilateral ECT at a seizure dosage at or above 388.

Hazardous substances events associated with the manufacturing of chemicals and allied products.

This report describes events involving the acute release of hazardous substances reported to the Hazardous Substances Emergency Events Surveillance (HSEES) system for 1993-2000. HSEES, maintained by the Agency for Toxic Substances and Disease Registry (ATSDR), collects data on the industries/services associated with events. This analysis focuses on fixed-facility events that occurred during the manufacturing of chemicals and allied products (i.