Ultracentrifugal subclasses of low and intermediate density lipoproteins.

Low density lipoprotein (LDL) and intermediate density lipoprotein (IDL) classes have been shown to be composed of discrete metabolic entities or subclasses. Present ultracentrifugal methods are unable to precisely determine these subclasses. A new analytical micro-ultracentrifugal method was developed that facilitates the determination of IDL and LDL subclasses and their F1.

High-dose carbonyl iron for iron deficiency anemia: a randomized double-blind trial.

To determine if high doses of oral iron could shorten the duration of therapy necessary to treat Fe deficiency anemia, high-dose Fe 600 mg three times per day (given as nontoxic carbonyl Fe) was compared with standard ferrous sulfate 60 mg Fe++ three times per day in a randomized, double-blind, 3-wk trial involving 36 female blood donors with mild Fe deficiency anemia. In animal studies, both forms of Fe have similar bioavailability when administered in equal amounts. High-dose carbonyl Fe was well tolerated with gastrointestinal side effects similar those observed with standard FeSO4 therapy.

Correlative design of electrophoretic and ultracentrifugal investigation of metabolic effects of probucol.

Probucol demonstrated a profound metabolic effect on the metabolism of plasma lipoproteins by lowering the levels of circulating low-density lipoproteins (LDL). This main metabolic phenomenon was usually accompanied with other substantial events in lipoprotein patterns. Their significance ought to be confirmed by more than one analytical method.

Ultracentrifugal study of metabolic effects of probucol: plasma lipoproteins in patients with proven coronary artery disease.

The primary metabolic effect of probucol is characterized by significant lowering of plasma low-density lipoproteins (LDL2) (p less than 0.01), frequently connected with a decrease in concentration of very-low density lipoproteins (VLDL) (p less than 0.05).

Lipoproteins in patients with proved coronary artery disease: qualitative and quantitative changes in agarose-gel electrophoretic patterns.

The relationship between the configuration of agarose-gel lipoprotein electrophoresis patterns and the extent of coronary artery disease (CAD) was studied in consecutive patients undergoing diagnostic coronary angiography. Three groups were identified: patients with normal coronary arteries (group 1), patients with minor luminal irregularities (group 2) and patients with significant coronary artery obstructions (group 3). Densitometric scans of the electrophoretograms were studied to determine the relative proportion of lipoproteins in each major fraction and the configuration of the pre-beta complex.