Exploring treatment-driven subclonal evolution of prognostic triple biomarkers: Dual gene fusions and chimeric RNA variants in novel subtypes of acute myeloid leukemia patients with KMT2A rearrangement.

Chromosomal rearrangements (CR) initiate leukemogenesis in approximately 50 % of acute myeloid leukemia (AML) patients; however, limited targeted therapies exist due to a lack of accurate molecular and genetic biomarkers of refractory mechanisms during treatment. Here, we investigated the pathological landscape of treatment resistance and relapse in 16 CR-AML patients by monitoring cytogenetic, RNAseq, and genome-wide changes among newly diagnosed, refractory, and relapsed AML. First, in FISH-diagnosed KMT2A (MLL gene, 11q23)/AFDN (AF6, 6q27)-rearrangement, RNA-sequencing identified an unknown CCDC32 (15q15.

Comprehensive Single-Cell Immune Profiling Defines the Patient Multiple Myeloma Microenvironment Following Oncolytic Virus Therapy in a Phase Ib Trial.

Purpose: Our preclinical studies showed that the oncolytic reovirus formulation pelareorep (PELA) has significant immunomodulatory anti-myeloma activity. We conducted an investigator-initiated clinical trial to evaluate PELA in combination with dexamethasone (Dex) and bortezomib (BZ) and define the tumor immune microenvironment (TiME) in patients with multiple myeloma treated with this regimen.

Patients And Methods: Patients with relapsed/refractory multiple myeloma (n = 14) were enrolled in a phase Ib clinical trial (ClinicalTrials.

Telocytes regulate macrophages in periodontal disease.

Telocytes (TCs) or interstitial cells are characterised in vivo by their long projections that contact other cell types. Although telocytes can be found in many different tissues including the heart, lung, and intestine, their tissue-specific roles are poorly understood. Here we identify a specific cell signalling role for telocytes in the periodontium whereby telocytes regulate macrophage activity.

A Proposal for Evaluating Psychosocial Trauma.

Background: The objective of the study was to design and validate the Psychosocial Trauma Scale (ETAPS) for assessing psychosocial consequences of collective violence. This instrument proposed the following dimensions: Pre-traumatic Situation, Destruction of Fundamental Beliefs, Intergroup Emotions, and Family and Community Destruction.

Method: A total of 382 people participated who had been affected by political violence: civil war in El Salvador, forced displacement from Colombia and state violence from Chile.

GLP1 analogues among patients with overweight or obesity during lockdown.

Background And Objectives: One of the potential negative effects of a lockdown are changes in dietary and lifestyle patterns, which can lead to weight gain. Our objective was to assess the changes on dietary habits and eating patterns in a lockdown situation and their impact on weight. We aimed to determine whether the treatment with GLP1 analogues (aGLP1) could impact on these parameters.

AlInN on Si (100) Solar Cells (x = 0-0.56) Deposited by RF Sputtering.

We investigate the photovoltaic performance of solar cells based on n-AlInN (x = 0-0.56) on p-Si (100) hetero-junctions deposited by radio frequency sputtering. The AlInN layers own an optical bandgap absorption edge tuneable from 1.

Silicone oil droplets in repackaged anti-vascular endothelial growth factors for intravitreal injections: In search of the main source of contamination.

Introduction: Repackaging of anti-vascular endothelial growth factors in polypropylene syringes lubricated with silicone oil for intravitreal use is associated with the presence of intravitreal silicone oil droplets. The objective of this study is to assess how the use of silicone-free syringes (for storage and/or administration) would reduce the amount of silicone oil droplets in the product to be administered.

Methods: Two 16 mL vials of bevacizumab were repackaged at the compounding pharmacy to obtain four sets of product, each consisting of three 1.

Cheating on Unproctored Internet Test Applications: An Analysis of a Verification Test in a Real Personnel Selection Context.

This study analyses the extent to which cheating occurs in a real selection setting. A two-stage, unproctored and proctored, test administration was considered. Test score inconsistencies were concluded by applying a verification test (Guo and Drasgow Z-test).

Assessing the Big Five with bifactor computerized adaptive testing.

Multidimensional computerized adaptive testing based on the bifactor model (MCAT-B) can provide efficient assessments of multifaceted constructs. In this study, MCAT-B was compared with a short fixed-length scale and computerized adaptive testing based on unidimensional (UCAT) and multidimensional (correlated-factors) models (MCAT) to measure the Big Five model of personality. The sample comprised 826 respondents who completed a pool with 360 personality items measuring the Big Five domains and facets.

Inferential Item-Fit Evaluation in Cognitive Diagnosis Modeling.

Research related to the fit evaluation at the item level involving cognitive diagnosis models (CDMs) has been scarce. According to the parsimony principle, balancing goodness of fit against model complexity is necessary. General CDMs require a larger sample size to be estimated reliably, and can lead to worse attribute classification accuracy than the appropriate reduced models when the sample size is small and the item quality is poor, which is typically the case in many empirical applications.

Calibrating a new item pool to adaptively assess the Big Five.

Background: Even though the Five Factor Model (FFM) has been the dominant paradigm in personality research for the past two decades, very few studies have measured the FFM adaptively. Thus, the purpose of this research was the building of a new item pool to develop a computerized adaptive test (CAT) for personality assessment.

Method: A pool of 480 items that measured the FFM facets was developed and applied to 826 participants.

Endophthalmitis after intravitreal injections. Incidence, management and prognosis.

Objective: To assess the rate of endophthalmitis after intravitreal injection (IVI) in a «clean room» of a single health centre, following the guidelines of the Spanish Vitreo-Retinal Society (SERV). An analysis was performed on the culture specimens, response to treatment, and final outcomes (guidelines).

Material And Methods: A retrospective, observational study was conducted on a consecutive case series of patients diagnosed with infectious endophthalmitis after IVI in a single health centre between 2010 and 2015.

Structural brain connectivity and cognitive ability differences: A multivariate distance matrix regression analysis.

Neuroimaging research involves analyses of huge amounts of biological data that might or might not be related with cognition. This relationship is usually approached using univariate methods, and, therefore, correction methods are mandatory for reducing false positives. Nevertheless, the probability of false negatives is also increased.

A multistage adaptive test of fluid intelligence.

Background: Multistage adaptive testing has recently emerged as an alternative to the computerized adaptive test. The current study details a new multistage test to assess fluid intelligence.

Method: An item pool of progressive matrices with constructed response format was developed, and divided into six subtests.

Electrical Characterization of Amorphous Silicon MIS-Based Structures for HIT Solar Cell Applications.

A complete electrical characterization of hydrogenated amorphous silicon layers (a-Si:H) deposited on crystalline silicon (c-Si) substrates by electron cyclotron resonance chemical vapor deposition (ECR-CVD) was carried out. These structures are of interest for photovoltaic applications. Different growth temperatures between 30 and 200 °C were used.

Study of association of CTLA4 gene variants to non-anterior uveitis.

This study was undertaken to investigate the possible genetic association of functional CTLA4 polymorphisms with susceptibility to non-anterior uveitis. Four hundred and seventeen patients with endogenous non-anterior uveitis and 1517 healthy controls of Spanish Caucasian origin were genotyped for the CTLA4 polymorphisms rs733618, rs5742909 and rs231775, using predesigned TaqMan(©) allele discrimination assays. PLINK software was used for the statistical analyses.

Specific association of IL17A genetic variants with panuveitis.

Background/aims: A pathogenic role of Th17 cells in uveitis has become clear in recent years. Therefore, in the present study, we aimed to evaluate the possible influence of the IL17A locus on susceptibility to non-anterior uveitis and its main clinical subgroups.

Methods: Five IL17A polymorphisms (rs4711998, rs8193036, rs3819024, rs2275913 and rs7747909), selected by tagging, were genotyped using TaqMan assays in 353 Spanish patients with non-anterior uveitis and 1851 ethnically matched controls.

Exploratory factor analysis in validation studies: uses and recommendations.

Background: The Exploratory Factor Analysis (EFA) procedure is one of the most commonly used in social and behavioral sciences. However, it is also one of the most criticized due to the poor management researchers usually display. The main goal is to examine the relationship between practices usually considered more appropriate and actual decisions made by researchers.

Application of cognitive diagnosis models to competency-based situational judgment tests.

Background: Profiling of jobs in terms of competency requirements has increasingly been applied in many organizational settings. Testing these competencies through situational judgment tests (SJTs) leads to validity problems because it is not usually clear which constructs SJTs measure. The primary purpose of this paper is to evaluate whether the application of cognitive diagnosis models (CDM) to competency-based SJTs can ascertain the underlying competencies measured by the items, and whether these competencies can be estimated precisely.

Optimal number of strata for the stratified methods in computerized adaptive testing.

Test security can be a major problem in computerized adaptive testing, as examinees can share information about the items they receive. Of the different item selection rules proposed to alleviate this risk, stratified methods are among those that have received most attention. In these methods, only low discriminative items can be presented at the beginning of the test and the mean information of the items increases as the test goes on.

Estimation of antioxidants dietary intake in wet age-related macular degeneration patients.

Aims: The aim of this study was to estimate the intake of antioxidant nutrients in wet age-related macular degeneration (AMD) patients, a degenerative and progressive disorder of the macula, which is the central part of the retina, associated with central vision loss.

Methods: A sample (n = 52, 78.9 ± 6.