Publications by authors named "O Zemskova"
ANALYSIS OF DNA METHYLATION CHANGES IN MANIFESTATION OF DIRECT AND RESCUE BYSTANDER EFFECTS.
Probl Radiac Med Radiobiol
December 2024
Objective: to investigate changes in DNA methylation in bystander and inducer cells during the manifestation ofdirect and rescue bystander effects.
Methods: Separate and co-cultivation of peripheral blood lymphocytes (PBL) of 10 conditionally healthy individuals; γ-quantum irradiation (IBL-237C emitter); modified comet electrophoresis method (Comet assay) under neutralconditions using the methylation-sensitive restriction enzyme HpaII; fluorescence microscopy with an automatedcomputer software system for analyzing the results; statistical methods.
Results: The level of DNA methylation in PBL was quantitatively assessed using DNA migration parameters inagarose gel: the length of the comet tail (in μm), the percentage of DNA in the tail part of the comet, and TailMoment (TM), which simultaneously takes into account both the amount of DNA in the tail part of the comet andthe length of the tail.
: This analysis aims to evaluate gender-specific differences in nutritional status, body weight changes, and their impact on overall survival (OS) in head and neck cancer (HNC) patients undergoing (chemo)radiotherapy (CRT). : Between 2018 and 2020, 61 HNC (17 female and 44 male) patients were prospectively recruited to receive curative (chemo)radiotherapy. Nutritional assessments included dietary questionnaire screenings and records, anthropometric methods (body mass index, BMI, body composition via bioelectrical impedance analysis (BIA)), and the determination of biomarkers like albumin and CRP.
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- This study investigates the impact of presurgical tumor growth and other clinical factors on the survival of glioblastoma (GBM) patients.
- A retrospective analysis of 98 adult GBM patients revealed that tumor growth rates did not significantly affect overall survival (OS) or progression-free survival (PFS).
- Key predictors of treatment outcomes included MGMT status, radiation dose, and the number of adjuvant cycles of temozolomide (TMZ), which can help inform personalized treatment plans.
Article Synopsis
- - The study focuses on identifying subgroups of glioblastoma (GBM) patients that might respond differently to two types of radiotherapy: standard radiotherapy (SRT) and hypofractionated radiotherapy (HRT), to facilitate personalized treatment decisions.
- - Researchers analyzed data from 159 patients, creating 48 subgroups based on five key factors (age, sex, surgical resection extent, chemotherapy, and functional status) to evaluate differences in cancer-specific survival (CSS) between the two treatment groups.
- - Results indicated that SRT was only better than HRT for CSS in three specific patient clusters, while the majority of the analyzed subgroups showed no significant differences in survival outcomes, suggesting the need for a tailored approach
Background/aim: The prognostic value of scoring instruments was described for newly diagnosed glioblastoma. This study investigated five instruments in patients with recurrent tumors.
Patients And Methods: Original Glasgow Prognostic Score (oGPS), modified GPS (mGPS), high-sensitivity mGPS (HS-mGPS), high-sensitivity oGPS (HS-oGPS), LabBM score, and 10 other factors were analyzed for progression-free survival (PFS) and overall survival (OS) in 51 patients.