[Fucolectins in diagnostic and neoplasm investigation].

Problems of practical application of fucose-binding lectins as selective histochemical markers and their diagnostical and prognostical value under different pathological states are considered. Special attention is paid to carbohydrate specificity of the most investigated and perspective fucolectins which are already applied in investigation of regularity of cell's functioning and differentiation. It is shown that fucose-specific lectins are useful diagnostic reagents in pathomorphological and clinical investigations.

[Studies of oligosaccharide specificity of perch roe fucolectin, using gold labeled neoglycoproteins].

The specificity of perch (Perca fluviatilis) roe fucolectin was studied using the protein dot blot technique, followed by detection with colloidal gold-labeled neoglycoproteins bearing human milk polysaccharides. The strongest binding was noted with the H type 1 pentasaccharide lacto-N-fucopentaose (LNFP I, Fuc alpha 1-2 Gal beta 1-3 GlcNAc beta 1-3 Gal beta 1-4Glc); the interaction with the H type 6 trisaccharide 2'-fucosyllactose (2-FL, Fuc alpha 1-2 Gal beta 1-4 Glc) was weaker. Binding of the perch lectin to the Lewis antigens (associated with tumors and embryonic tissues) was also studied.