New synthesis of oligosaccharides modelling the M epitope of the O-polysaccharide.

Brucellosis is a dangerous zoonotic disease caused by bacteria of the genus . Diagnosis of brucellosis is based on the detection in animal and human sera of antibodies to the O-polysaccharide of lipopolysaccharide. The currently employed serodiagnosis of brucellosis relies on the use of the O-polysaccharide as a diagnostic antigen.

Russian collection of vaccine strains: annotation, implementation and genomic analysis.

Over the past 10 years, immunization of cattle in Russia has been performed using vaccines from strains 82, 19 and 75/79. To prevent brucellosis in small ruminants, two vaccines have been used, from the strain REV-1 and the strain 19; note that twice as many animals have been immunized with the former vaccine than with the latter vaccine. The disadvantage of using these preparations is the formation of prolonged post-vaccination seropositivity, which is especially pronounced in animals after immunization with vaccines from strain 19 and strain REV-1.

The changes of some bioregulators and gas transmitters in patients with hypertonic disease under the influence of physical loading.

Objective: Introduction: Nowadays arterial hypertension is supposed to be a pathogenetic factor of numerous cardiovascular diseases (CVD) and 18% cases of the preterm death. Due to the data of the American Heart Association in 77% of patients, who had stroke, the arterial pressure overexceeded 140/90 mm Hg. In Ukraine, according to the epidemiologic study more than 1/3 of the population have increased arterial pressure.

Changes in the antioxidant system and level of proinflammatory cytokin IL-1beta in the blood patients of sufferi.

Diabetes mellitus type 1 provokes the development of the oxidative stress, accompanied by the increased level of TBA-active products, activation of NO-synthases and increased production of nitric oxide. Activation of glutathione reductase, decrease of the glutathione peroxidase and superoxide dismutases activity was observed. This shows a specific disturbance in the functioning of the antioxidant defense system and the augmentation in the concentration of one of the major first line reacting cytokin (IL-1beta).

Development and characterization of a modified Komarov's bullet for ballistic delivery of live Brucella abortus strains 82 and 19 to cattle and bison.

In this study a modified Komarov's bullet was developed to remotely deliver live brucellosis vaccines (Brucella abortus 82 and Brucella abortus 19). After modification, the bullet payload could carry the desired dose (10(11)CFU) of vaccine. As the bullet components were toxic to the live bacteria, a special protective coating was developed for the bullet inner surface that maintained vaccine viability.