A second species of Protistolophus Short from Peru (Coleoptera: Hydrophilidae: Hydrophilinae).

A second species of the enigmatic hydrophilid genus Protistolophus Short, 2010 is described from the Cordillera Escalera in northwest Peru (Departamento de San Martín). Most specimens were collected in association with brooklets in primary forest at ca. 1300 m a.

A preliminary checklist of the Coccinellidae of Bhutan (Insecta: Coleoptera).

An annotated distributional checklist of the Coccinellidae of Bhutan is presented, enumerating 91 species, 17 of which are here recorded for the first time: Shirozuella tibetina Wang, Ge Ren, 2012, Jauravia limbata (Motschulsky, 1858), Scymnus bourdilloni (Kapur, 1958), Illeis confusa Timberlake, 1943, Calvia albida Bielawski, 1972, Harmonia expalliata Sicard, 1913, H. octomaculata (Fabricius, 1781), H. sedecimnotata (Fabricius, 1801), Micraspis allardi (Mulsant, 1866), M.

Rewilding with large herbivores: Positive direct and delayed effects of carrion on plant and arthropod communities.

Carrion of large animals is an extremely nutrient rich, ephemeral resource that is essential for many species, but is scarce in the anthropogenic Western-European landscape due to legislative restrictions. Rewilding, a novel conservation strategy that aims at restoring natural processes with minimal human intervention, is increasing in popularity and could lead to increased carrion availability in the landscape. It is therefore important to understand the effects of carrion on biodiversity.

Sucrose prevents up-regulation of senescence-associated genes in carnation petals.

cDNA microarrays were used to characterize senescence-associated gene expression in petals of cut carnation (Dianthus caryophyllus) flowers, sampled from anthesis to the first senescence symptoms. The population of PCR fragments spotted on these microarrays was enriched for flower-specific and senescence-specific genes, using subtractive hybridization. About 90% of the transcripts showed a large increase in quantity, approximately 25% transiently, and about 65% throughout the 7 d experiment.

Combined transcriptome and proteome analysis identifies pathways and markers associated with the establishment of rapeseed microspore-derived embryo development.

Microspore-derived embryo (MDE) cultures are used as a model system to study plant cell totipotency and as an in vitro system to study embryo development. We characterized and compared the transcriptome and proteome of rapeseed (Brassica napus) MDEs from the few-celled stage to the globular/heart stage using two MDE culture systems: conventional cultures in which MDEs initially develop as unorganized clusters that usually lack a suspensor, and a novel suspensor-bearing embryo culture system in which the embryo proper originates from the distal cell of a suspensor-like structure and undergoes the same ordered cell divisions as the zygotic embryo. Improved histodifferentiation of suspensor-bearing MDEs suggests a new role for the suspensor in driving embryo cell identity and patterning.