[The binding of furosemide and ethacrynic acid with different types of biological membranes].

The study was undertaken to examine the effects of furosemide and ethacrynic acid on the fluorescence of 1,8-AHC in the suspension of erythrocytic membranes and lumenal and basolateral membranes of the rat renal epithelium. Ethacrynic acid was demonstrated to decrease probe luminescence in all three types of biological membranes. Furosemide failed to affect 1,8-AHC fluorescence in the suspension of blood ghosts and basolateral membranes of the renal epithelium and enhances probe fluorescence in the suspension of luminal membranes.

[Study of the binding of diuretics by serum proteins according to changes in tryptophan fluorescence].

The effect of diuretics on tryptophan fluorescence of blood serum proteins divided by electrophoresis was studied. It was shown that ethacrynic acid produces the most significant extinction of tryptophan fluorescence in albumin fraction and novurit in globulin fraction. Furosemide possessing a high affinity for all three obtained fractions of protein does not exhibit a preferential binding to one or another of these fractions.

[Diuretic binding with biological membranes studied by means of a fluorescent probe].

The binding of furosemide, novurit and ethacrynic acid with human serum albumin, phospholipid liposomes, mitochondria and plasmatic membranes of rat kidneys was studied by the fluorescent probe 1-anilino naphthalene-8-sulfonate (1,8-ANS) method. It is shown that ethacrynic acid has high associative constants with serum albumin molecule and plasmatic membranes. Novurit has the greatest affinity with rat kidney mitochondria.