[Improvement in methodology of occupational risk evaluation].

The authors suggest to supplement a method evaluating individual occupational risk with tree blocks of occupational and non-occupational risk factors: index of sanitary legislation requirements fulfilment according to experts evaluation; lifestyle index with individual biologic and behavioral risk factors consideration; index of social well-being, considering social economic and social psychologic risk factors at workplace.

[Induction of intracellular endonuclease synthesis in Serratia marcescens by agents suppressing DNA replication].

Endonuclease synthesis in Serratia marcescens was studied in the presence of agents selectively suppressing DNA biosynthesis: nalidixic acid, mitomycin, hydroxyurea and thymine limitation. All the agents suppressing DNA replication induced exocellular endonuclease biosynthesis irrespective of their action mechanism. The greatest inducing effect was exerted when the agents were added to cells in the late exponential phase.

[Comparative study of the energy metabolic enzymatic activity of pigmented and pigment-free strains of Serratia marcescens].

The activity of enzymes involved in energy metabolism was investigated in the pigmented strain of Serratia marcescens and in its pigmentless variant with an elevated activity of nuclease. The pigmentless strain was found to exhibit a higher specific activity of several enzymes participating in glycolysis, the pentose phosphate cycle, and the citric acid cycle. The pigmentless strain accumulated lesser amounts of lactic acid in the medium and was characterized by the negative Voges--Proskauer reaction.

[Various physiological aspects of Serratia marcescens pigmented strains and their pigmentless variants with an elevated nuclease activity].

Some aspects of physiology of Serratia marcescens pigmentless variants with elevated nuclease activity were studied. The variants are characterized by a higher respiration rate when glucose, glycerol, inositol or maltose are used as an energy substrate, a higher respiration quotient, a lower growth rate, a lower economic coefficient, and a lower thermogenesis. The growth of Serratia marcescens pigmentless strains is presumed to be unbalanced.