[The characteristics of the phospholipid composition of the pulp in the milk teeth].

Qualitative and quantitative phospholipid composition of porcine deciduous teeth pulp was studied by microthin-layer chromatography. Basing on the differences in the levels of individual phospholipids in the pulp of deciduous and permanent teeth, the authors come to a conclusion on the inadequate structural organization and functional activity of the pulp of deciduous teeth.

[The characteristics of lectin binding to the surface of differentiated neuroblastoma C 1300 cells].

Peculiarities of the binding of lectins of castor bean, cochlea, soya bean, lime bean and wheat germs to the surface of differential neuroblastoma C 1300 N 18 cells have been studied using the method electron cytochemistry. It is found that the quantity of the bound lectin conjugates with colloidal gold on the surface of differentiated cells varied considerably from that on the surface of nondifferentiated cells.

[Identification and properties of insulin receptors in neuroblastoma C 1300 N 18 cells in various functional states].

The presence of insulin receptors in neuroblastoma C 1300 N18 cells was shown by the method of the [125I]insulin binding with cells as well as by the electron-cytochemical methods based on the analysis of the binding of colloid gold-labelled insulin and of agglutinin of wheat embryos with the surface of plasma membrane. It is established that the number and distribution of insulin receptors depend on the functional state of cells. Expression of receptors on the membrane increases after 5'-deoxyuridine-induced differentiation of cells.

[Evaluation of the distribution of lectin receptors on the surface of tumor and transformed cells using methods of variation statistics].

Statistical procedures were used to estimate lectin receptor distribution on the surface of ascite lymphoma cells, neuroblastoma C-1300 cells and of transformed human T- and B-derived lymphoid cell lines. Relationships between the arithmetic means and mean square variances for sample populations from each cell and ferritin- or colloidal gold-lectin combination were used to define four types of topographical distributions: uniform-ordered, uniform-random, random and clustered.

[Localization of lectin receptors on the surface of C1300 neuroblastoma cells].

D-mannose, D-galactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine and L-fucose which are sugar determinants of receptors were found on the surface of neuroblastoma cells by means of four carbohydrate-specific lectin groups. Labeling of lectins was performed by horseradish peroxidase, ferritin and colloidal gold. Peculiarities of the lectin receptors distribution on the surface of immature neuroblastoma cells were detected.